Novel multiresistance-mediating integrative and conjugative elements carrying unusual antimicrobial resistance genes in <i>Mannheimia haemolytica</i> and <i>Pasteurella multocida</i>

Schink, Anne‐Kathrin; Hanke, Dennis; Semmler, Torsten; Brombach, Julian; Bethe, Astrid; Lübke‐Becker, Antina; Teske, Kinga; Müller, Kerstin; Schwarz, Štefan

doi:10.1093/jac/dkac116

Cited by 13 publications

(7 citation statements)

References 8 publications

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“…ICEs were detected in 86.7% of the ST79 strains harboring ARGs (Figure 3). Within the ST79 cluster, two isolates from this study that harbored ICE, 720CV and 189CV, had 10 SNP differences between them and a maximum of 27 SNP differences with the German bovine isolate (GCA_020971725.1) (Figure 3) that harbored ICE Tn7407 [26]. By mapping the Illumina short-read data from the two studied isolates against the Tn7407 ICE, a near-complete match was found, with an identity of 99.2% and 100% coverage of the ICE.…”

Section: Phylogenetic Relatedness Virulence-associated Genes Mobile G...mentioning

confidence: 86%

“…For two isolates from this study in which a putative ICE was detected (see Section 3), similarity to ICE Tn7407, which was previously described from a German isolate (GenBank accession no. CP087380; [26]) was assessed. Illumina short reads were mapped against Tn7407 using the BWA v0.7.17 tool [27].…”

Section: Detection Of Virulence-associated Genes Antimicrobial Resist...mentioning

confidence: 99%

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Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Calderón Bernal,

Serna,

García Muñoz

et al. 2023

Animals

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The aim of this study was to investigate the possible genotypic differences between commensal Pasteurella multocida isolates from apparently healthy animals (AHA) at the time of entry to feedlots and those from BRD-affected animals (BRD-AA). A total of 20 batches of beef calves in seven feedlots were followed-up during the fattening period. P. multocida was isolated from 28.1% of AHA and 22.9% of BRD-AA. All isolates belonged to the A: L3 genotype. Most isolates from clinical cases (81.0%) grouped into a PFGE cluster were significantly associated with BRD cases (OR, 24.9; 95% CI, 6.4–96.2). The whole genomes of 14 isolates representative of the pulsotypes most frequently detected in BRD-AA and AHA were sequenced and compared with 53 bovine genomes belonging to the identified ST13, ST79, and ST80 genotypes for a global comparison. No differences were found in the virulence-associated gene content between sequence types (STs) globally or between BRD-AA and AHA isolates in this study. Significantly, ST79 isolates harbored ARGs, conferring resistance to different antimicrobials, including macrolides and tetracyclines, which are commonly used for the treatment of BRD. Two Spanish ST79 isolates carried an ICE highly similar to ICE Tn7407, which was recently detected in Germany, suggesting that ST79 P. multocida isolates in Europe and North America may be associated with different ICEs.

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Section: Phylogenetic Relatedness Virulence-associated Genes Mobile G...mentioning

confidence: 86%

Section: Detection Of Virulence-associated Genes Antimicrobial Resist...mentioning

confidence: 99%

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Calderón Bernal,

Serna,

García Muñoz

et al. 2023

Animals

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“…That study reported MDR rates of 13.9% and 5.1%, while rates of pan-susceptibility were only 15.1% and 12.1% in isolates of P. multocida and M. haemolytica, respectively [60]. Another recent study identified a novel high-level macrolide resistance determinant in M. haemolytica, further limiting treatment options for BRD [61]. New antimicrobial agents that target P. multocida and M. haemolytica would consequently be highly advantageous.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antibacterial Activity of Microbial Natural Products against Bacterial Pathogens of Veterinary and Zoonotic Relevance

Barth,

Preussger,

Pietschmann

et al. 2024

Antibiotics

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Antimicrobial resistance (AMR) is considered one of the greatest threats to both human and animal health. Efforts to address AMR include implementing antimicrobial stewardship programs and introducing alternative treatment options. Nevertheless, effective treatment of infectious diseases caused by bacteria will still require the identification and development of new antimicrobial agents. Eight different natural products were tested for antimicrobial activity against seven pathogenic bacterial species (Brachyspira sp., Chlamydia sp., Clostridioides sp., Mannheimia sp., Mycobacterium sp., Mycoplasma sp., Pasteurella sp.). In a first pre-screening, most compounds (five out of eight) inhibited bacterial growth only at high concentrations, but three natural products (celastramycin A [CA], closthioamide [CT], maduranic acid [MA]) displayed activity at concentrations <2 µg/mL against Pasteurella sp. and two of them (CA and CT) also against Mannheimia sp. Those results were confirmed by testing a larger collection of isolates encompassing 64 Pasteurella and 56 Mannheimia field isolates originating from pigs or cattle, which yielded MIC90 values of 0.5, 0.5, and 2 µg/mL against Pasteurella and 0.5, 4, and >16 µg/mL against Mannheimia for CA, CT, and MA, respectively. CA, CT, and MA exhibited higher MIC50 and MIC90 values against Pasteurella isolates with a known AMR phenotype against commonly used therapeutic antimicrobial agents than against isolates with unknown AMR profiles. This study demonstrates the importance of whole-cell antibacterial screening of natural products to identify promising scaffolds with broad- or narrow-spectrum antimicrobial activity against important Gram-negative veterinary pathogens with zoonotic potential.

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“… 1 , 2 In the recent past, North America has seen a rapid rise in resistant variants of the two most predominant BRD pathogens, Pasteurella multocida and Mannheimia haemolytica , resulting in infections resistant to first- and second-line antibiotics prescribed for BRD. 3–7 Acquisition of integrative conjugative elements (ICEs) was linked to this rapid upsurge of the resistance problem in North America 8–10 and Europe; 11 however, such elements are yet to be reported in Australia.…”

Section: Introductionmentioning

confidence: 99%

Identification and evolution of ICE-PmuST394: a novel integrative conjugative element in Pasteurella multocida ST394

Roy Chowdhury,

Alhamami,

Venter

et al. 2024

Journal of Antimicrobial Chemotherapy

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Background The emergence of macrolide and tetracycline resistance within Pasteurella multocida isolated from feedlot cattle and the dominance of ST394 in Australia was reported recently. Objectives To establish the genetic context of the resistance genes in P. multocida 17BRD-035, the ST394 reference genome, and conduct a molecular risk assessment of their ability to disperse laterally. Methods A bioinformatic analysis of the P. multocida 17BRD-035 genome was conducted to determine if integrative conjugative elements (ICEs) carrying resistance genes, which hamper antibiotic treatment options locally, are in circulation in Australian feedlots. Results A novel element, ICE-PmuST394, was characterized in P. multocida 17BRD-035. It was also identified in three other isolates (two ST394s and a ST125) in Australia and is likely present in a genome representing P. multocida ST79 from the USA. ICE-PmuST394 houses a resistance module carrying two variants of the blaROB gene, blaROB-1 and blaROB-13, and the macrolide esterase gene, estT. The resistance gene combination on ICE-PmuST394 confers resistance to ampicillin and tilmicosin, but not to tulathromycin and tildipirosin. Our analysis suggests that ICE-PmuST394 is circulating both by clonal expansion and horizontal transfer but is currently restricted to a single feedlot in Australia. Conclusions ICE-PmuST394 carries a limited number of unusual antimicrobial resistance genes but has hotspots that facilitate genomic recombination. The element is therefore amenable to hosting more resistance genes, and therefore its presence (or dispersal) should be regularly monitored. The element has a unique molecular marker, which could be exploited for genomic surveillance purposes locally and globally.

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Novel multiresistance-mediating integrative and conjugative elements carrying unusual antimicrobial resistance genes in Mannheimia haemolytica and Pasteurella multocida

Cited by 13 publications

References 8 publications

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

In Vitro Antibacterial Activity of Microbial Natural Products against Bacterial Pathogens of Veterinary and Zoonotic Relevance

Identification and evolution of ICE-PmuST394: a novel integrative conjugative element in Pasteurella multocida ST394

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