Novel N–Br Bond-Containing N-Halamine Nanofibers with Antibacterial Activities

Abstract

N-Halamine compounds have attracted great attention because they are recognized as promising antibacterial agents to control microbial contamination; however, most of the research interests were focused on N-halamines that contain N–Cl bond(s) rather than N–Br bond(s). In this contribution, we report the facile fabrication of N–Br bond-containing N-halamine nanofibers using the electrospinning method for antibacterial usages. The as-produced N–Br bond-containing N-halamine nanofibers (i.e., DBDMH/PAN nanofibers) comprise an antibacterial component of 1,3-dibromo-5,5-dimethylhydantoin (DBDMH) and a support component of polyacrylonitrile (PAN). When systematic characterizations were carried out, the as-obtained DBDMH/PAN nanofibers were proven to exhibit well-defined fiber-like morphology and be highly efficient in the killing of the selected model bacteria (Escherichia coli). Their morphology and size could be well governed by tuning the concentration of electrospinning precursor and the mass ratio of PAN to DBDMH. The antibacterial mechanism of the DBDMH/PAN nanofibers and their stabilities under dry, wet, and bacterial conditions were confirmed as well. Facile synthesis and antibacterial activity allow the feasibility of the final N–Br bond-containing N-halamine nanofibers for antibacterial-related clinical applications in practice. Our work highlights the development of the N–Br bond-containing N-halamine nanofibers as promising antibacterial agents for biomedical applications.

Antibacterial N-halamine fibrous materials

“…It was found that the release rate of N -halamine precursor was low even conducting with vigorous agitation, suggesting that the materials inactivate bacteria via contact killing instead of release killing. Thereafter, Bai et al [ 50 ] investigated the antibacterial behavior of the as-prepared N–Br bond-containing N -halamine nanofibers using inhibition zone test, the appearance of the distinct aseptic ring around the samples validated the release killing mode. Then they tested release rate of active bromine dissociated from the N -halamine nanofibers under different conditions.…”

Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study

“…At the present scenario, due to toxicity and multidrug resistance developed by the bacteria against conventional antibiotics, much attention has been given to new classes of antimicrobials [16]. The advanced wound dressings are infused with antimicrobials such as honey [17,18], medicinal plant extracts and their bioactive compounds [19,20,21,22], nanoparticles [23,24,25], essential oils [26,27,28], polycatecholamines [29] and cationic polymers [30].…”

Electrospun Sesbania Gum-Based Polymeric N-Halamines for Antibacterial Applications

“…Antibacterial testing was performed using the colony-counting method [45], with Escherichia coli ( E. coli , 8099, a typical Gram-negative bacterium) and Staphylococcus aureus ( S. aureus , ATCC 6538, a typical Gram-positive bacterium) as the two model bacteria. Typically, E. coli and S. aureus were grown overnight at 37 °C in Luria–Bertani (LB) medium, then the bacterial cells were harvested by centrifugation, washed with phosphate-buffered saline, and diluted to concentrations of 1 × 10 6 CFU/mL.…”