Novel poly(ethylene‐co‐acrylic acid) nanofibrous biomaterials for peptide synthesis and biomedical applications

Abstract

Poly(ethylene-co-acrylic acid) (PE-co-AA) fibers in sizes of 200-500 nm were prepared by using a novel melt-extrusionextraction fabrication process. The thermoplastic nanofibers could be controllably dispersed and reassembled by a novel solvent exchange filtration method. The dispersed PE-co-AA nanofibers possess active surface areas and could directly conduct chemical reactions on surfaces. Surface modifications and organic synthesis on the nanofibers were proven effective and controllable after the dispersion. Multistep synthesis of biomolecules, such as peptide ligand HWRGWV against Fc portion of human IgG, was successful. The surface-anchored ligand has shown bioactivity through selective binding to and staining by human IgG-alkaline phosphatase conjugate. Another peptide, LXY3, a selective cyclic peptide ligand against a3b1 integrin of MDA-MB-231 breast cancer cells, was also prepared on the surfaces of the dispersed nanofibers. The results showed that MDA-MB-231 cells were able to specifically bind to and grow on surfaces of the nanofibers that were functionalized with LXY3.

Aggregation of poly(acrylic acid)-containing elastin-mimetic copolymers

“…25 PAA has enabled electrostatic association and covalent conjugation of biomolecules through its carboxylic acid functional group, in brush, micelle and ber structures. 25,26,66,67 It is also commonly used in amphiphilic systems to form various architectures, [68][69][70][71] and these architectures can be varied based on the PAA's ability to swell in solution due to pH-responsiveness. 72 The ability of PAA to form hydrogen bonds is also of interest, and interpolymer complexes (IPC) of PAA and poly(ethylene oxide) (PEO) have previously been formed, 73,74 and PAA-hydroxypropyl cellulose systems have been used to form nanoparticles through hydrogen bonding.…”

Preparation and photo-oxidative functions of poly(ethylene-co-methacrylic acid) (PE-co-MAA) nanofibrous membrane supported porphyrins

“…The quantification of accessible primary amine loading on the membrane surfaces was tested by Fmoc analysis. 21,22 Specifically, 5 mmol Fmoc-Gly-OH in 20 mL DMF was activated by equal amounts of DIC and HOBt for 30 min at room temperature, followed by adding 50 mg of aminated PE-co-MAA nanofibrous membrane. The mixture was shaken overnight to couple all accessible primary amine on membrane surfaces with N-terminal protected amino acid.…”

Surface zwitterionically functionalized PVA-co-PE nanofiber materials by click chemistry

“…Functional nanobers, especially the nanober materials with tunable surface chemical structures, have attracted considerable research interest in biomedical and biotechnological applications, [1][2][3][4][5][6][7] such as biosensors, 8,9 affinity membrane for biomolecules separation, [10][11][12] tissue engineering, 13,14 wound dressing 15 as well as drug delivery. 16 Nonspecic adhesions of biomolecules and microorganism on nanober surfaces, however, oen deteriorate the performance or functions of various biomedical and biotechnological devices.…”