Novel porcine rotavirus of genotype P[27] shares new phylogenetic lineage with G2 porcine rotavirus strain

Khamrin, Pattara; Maneekarn, Niwat; Peerakome, Supatra; Chan-it, Wisoot; Yagyu, Fumihiro; Okitsu, Shoko; Ushijima, Hiroshi

doi:10.1016/j.virol.2006.12.004

Cited by 106 publications

(102 citation statements)

References 41 publications

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“…We detected the recently described new rotavirus genotype P[27] in 11.1 % of all positive samples. It is interesting that, in this and other studies, the P[27] genotype was found in four G/P combinations: G1, G2-like, G4 (this study) and G5 (Khamrin et al, 2007;Martella et al, 2007;Steyer et al, 2007a). The rotavirus P[27] genotype strains (CMP034, 344/04-1 and P21-5) independently detected by these three author groups showed high genetic variability, as they shared only 89-90.8 % VP4 amino acid sequence identity within this genotype.…”

Section: Discussionsupporting

confidence: 59%

“…The most prevalent rotavirus genotypes in pigs were G3P[6] (15 samples, 18.5 %), G4P[6] (ten samples, 12.3 %) and G5P [7] (11 samples, 13.6 %). The recently recognized rotavirus genotype P[27] (Khamrin et al, 2007;Martella et al, 2007;Steyer et al, 2007a) was found in nine (11.1 %) samples and was combined with three different G types: G1, G2 and G4 (Table 4).…”

Section: Incidence Of Group a Rotaviruses In Human And Animal Stool Smentioning

confidence: 99%

“…As both epitopes are important for host immunity, eliciting neutralizing antibodies, a dual classification system for rotaviruses is used. To date at least 15 G and 27 P genotypes have been characterized using molecular techniques (Estes, 2001;Hoshino et al, 2002;Khamrin et al, 2007;Liprandi et al, 2003;Martella et al, 2003Martella et al, , 2006aMartella et al, , 2007McNeal et al, 2005;Rahman et al, 2005;Rao et al, 2000;Steyer et al, 2007a). Some of these genotypes have been found only in certain animal species or in humans and appear to be host-restricted.…”

Section: Introductionmentioning

confidence: 99%

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Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Steyer

Poljšak-Prijatelj

Barlič–Maganja

et al. 2008

Journal of General Virology

118

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A surveillance of human, porcine and bovine rotaviruses was carried out in Slovenia in 2004 and 2005. Stool samples were collected from a total of 406 pigs (373 from asymptomatic animals), 132 cattle (126 from asymptomatic animals) and 241 humans (all with diarrhoea), tested for group A rotaviruses using RT-PCR and analysed by sequencing. The aims of the study were to determine the incidence of asymptomatic rotavirus infection in animals, to look for evidence of zoonotic transmission and to detect reassortment among rotaviruses. The rates of asymptomatic shedding of rotaviruses in pigs and cattle were 18.0 % (67/373) and 4.0 % (5/126), respectively. Evidence for zoonotic transmission was detected in one human rotavirus strain, SI-MB6, with the G3P[6] genotype combination, as the nucleotide and predicted amino acid sequences of the VP6, VP7, VP8* and NSP4 genes of strain SI-MB6 and of porcine strains showed high nucleotide and amino acid sequence identity. Two porcine rotavirus strains carried VP7 of probable human origin, suggesting an interspecies reassortment event in the past.

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Section: Discussionsupporting

confidence: 59%

Section: Incidence Of Group a Rotaviruses In Human And Animal Stool Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Steyer

Poljšak-Prijatelj

Barlič–Maganja

et al. 2008

Journal of General Virology

118

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“…E1 is the predominant genotype in most parts of the world followed by E2. Some other uncommon genotypes like E6 and E9 have also been reported from a few geographical areas (Khamrin et al, 2007;Rehman et al, 2007).…”

Section: Genotypes and Dendogrammentioning

confidence: 95%

Molecular characterization of the rotavirus enterotoxin NSP4 gene of strains causing diarrhoea in children aged 0-5 years in northern India

Srivastava¹,

Jain²,

Khan³

et al. 2015

J App Pharm Sci

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Non structural protein 4 (NSP4) gene of Rotavirus encodes a multifunctional protein which has significant role in viral multiplication and pathogenesis of acute watery diarrhoea associated with rotaviral gastroenteritis. It is known as the first viral enterotoxin and mutations of the gene have been linked to altered pathogenesis. This study was planned to ascertain the genotypes and genetic variations of NSP4 gene in the rotavirus strains prevalent in this area. We collected consecutive diarrhoeal stools from equal no of children aged under five years hospitalized with diarrhoea in a period from January 2010 to June 2012 and tested them for rotavirus antigen by ELISA. NSP4 gene was amplified by RT-PCR and subsequently sequenced (Big-Dye terminator kit using 3130 ABI, Genetic analyzer) and genotyped by Rotavirus C software. Of the 260 samples, 58(22.3%) samples were positive by ELISA. We were able to amplify NSP4 gene by RTPCR from 45 strains of which 35 amplicons were selected for sequencing. Total 25(71.4%) strains belonged to genotype E1, 6 (17.1%) strains to genotype E2 and 4 (11.4%) matched with the genotype E6. Sequence analysis revealed changes in the nucleotides causing punctate mutations in the conserved regions, the Inter species variable domain (ISVD) and the enterotoxin region (amino acid 114-135). On evolutionary analysis of 33 strains amino acid at position 131 was found under positive selection.

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“…These antigens are responsible for conferring immunity and hence are a target for vaccine development. Although 15 different G serotypes and 27 P genotypes [8][9][10][11] have been detected in humans, serotypes G1P [8], G2P [4], G3P [8], G4P [8] and G9P [8] are thought to be important causes of diarrhea in infants and young children worldwide. 12 The human astroviruses have been identified as one of the most frequent causes of infantile gastroenteritis, second in incidence only to rotavirus.…”

Section: Introductionmentioning

confidence: 99%

Epidemiology of rotavirus and astrovirus infections in children in Northwestern Nigeria

et al. 2008

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Background: Recent estimates attribute 527 000 deaths in children less than five years of age to rotavirus diarrhea annually, with 145 000 occurring in sub-Saharan Africa. Human astroviruses have been identified as one of the most frequent causes of infantile diarrhea, second in incidence only to rotavirus. This study was conducted to determine the prevalence of rotavirus and astrovirus and also to establish the circulating strains of rotavirus in a community in Nigeria where most diarrheic patients do not visit clinics or health care centers. Methods: A total of 154 stool samples (134 diarrheic and 20 non-diarrheic) were collected from infants and young children less than 5 years of age from January-March 2002. Samples were obtained by house-to-house visit in randomly selected districts in Zaria, Northwestern Nigeria. The samples were screened for rotavirus and astrovirus antigens using commercially available Enzyme Linked Immunosorbent Assay (ELISA) kits. All positive group A rotavirus samples were further subjected to VP6 sub-group ELISA, Polyacrylamide gel electrophoresis (PAGE) to determine their RNA electropherotypes and Reverse transcription polymerase chain reaction (RT-PCR) to determine their VP7 and VP4 genotypes. Results: Rotavirus and astrovirus antigens were detected in 9% (12) and 5% (7) of the 134 diarrheic stool samples respectively. No viral antigen was detected in the non-diarrheic stools. Rotavirus infection was more common in younger children than astrovirus infection. VP6 sub-group II specificity (58.3%), long RNA electropherotypes (41.6%), VP7 genotype G1 (33.3%) and VP4 genotype P [6] (33.3%) were the most common strains in circulation at that time in the community. Of significance is the fact that a large proportion of the rotavirus strains in circulation could not be assigned either a VP6 subgroup or RNA electrophoretic pattern probably as a result of low viral load. Conclusion: In this community-based study, rotavirus and astrovirus were significantly associated with diarrhea. However, the prevalence of rotavirus infection among children appears to be low while that of astrovirus falls in the range seen in hospital-based studies around the continent. Rotavirus, astrovirus, diarrhoea, children, community, Nigeria Résume Contexte: Des estimations récentes attribuent 527 000 décès annuels d'enfants de moins de 5 ans à la diarrhée par rotavirus, parmi lesquels 145 000 surviennent en Afrique Sub Saharienne. Les astrovirus humains ont été identifiés comme l'une des causes les plus fréquentes de diarrhée infantile, en second lieu viennent les atrovirus. Cette étude a été menée pour déterminer la prévalence des rotavirus et astrovirus et établir les souches circulantes de rotavirus dans une population du Nigéria ou la plupart des patients diarrhéiques ne consultent pas en clinique ni les centres de santé. Key words: Epidemiology of Rotavirus and Astrovirus infection in Nigerian children. Aminu M. et al. Page | 169Méthode: Un total de 154 échantillons de selles (134 diarrhéiques et 20...

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Novel porcine rotavirus of genotype P[27] shares new phylogenetic lineage with G2 porcine rotavirus strain

Cited by 106 publications

References 41 publications

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Molecular characterization of the rotavirus enterotoxin NSP4 gene of strains causing diarrhoea in children aged 0-5 years in northern India

Epidemiology of rotavirus and astrovirus infections in children in Northwestern Nigeria

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