1996
DOI: 10.1099/0022-1317-77-6-1129
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Novel pre-C/C gene mutants of hepatitis B virus in chronic active hepatitis: naturally occurring escape mutants

Abstract: We have analysed serum samples taken from hepatitis B virus (HBV) e antigen (HBeAg)-positive and HBeAgnegative chronic active hepatitis (CAH) patients by PCR using primers spanning the pre-core/core (C) and pre-S1/$20RFs. Nucleotide sequence analysis showed that among 18 HBV-infected CAH patients, 11 had virus with a G to A mutation (nucleotide 1896; leading to the formation of a stop codon) and one patient also had virus with an additional G to A mutation three nucleotides downstream (nucleotide 1899). HBV fr… Show more

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Cited by 26 publications
(21 citation statements)
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“…In addition to mutants 38 and 60 ( Fig. 1 and 2), a highly frequent naturally occurring mutation at codon 5 (P5T) was also included in this study (14,18,22). In addition to P5T, several other codon 5 mutants (P5S and P5A) were constructed and characterized in parallel.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…In addition to mutants 38 and 60 ( Fig. 1 and 2), a highly frequent naturally occurring mutation at codon 5 (P5T) was also included in this study (14,18,22). In addition to P5T, several other codon 5 mutants (P5S and P5A) were constructed and characterized in parallel.…”
Section: Resultsmentioning
confidence: 99%
“…14,15,18,28,29,36). Finally, a very frequent mutation of HBcAg occurs at codon 5, changing a conserved proline (P) to a threonine (T) (1,2,6,11,13,14,18,22,33,38). While the most common mutation found at HBcAg codon 5 is from P to T, other less frequent changes have also been reported, such as proline to serine (S).…”
mentioning
confidence: 99%
“…Interestingly the C-terminus of the core-protein (aa 150-171) seems to tolerate mutations easily in spite of its replicative functions [12]. The Ser-169 frequently was changed into Pro.…”
Section: Mutations Of the Core-regionmentioning
confidence: 99%
“…These experiments might allow the conclusion that mutants with additional Cys or change of the Cys-61 can alter the conformation of the HBeAg or the HBcAg. The newly developed proteins could no longer be recognized by antibodies [12,107].…”
Section: Mutations Of the Precore-regionmentioning
confidence: 99%
“…In the literature, out of 65 sequences containing 97L mutations, 10 (15.4%) have concurrent P5T mutations (1,2,5,9,12,13,15,25,27,28). Previously, we reported a compensatory core mutation, P130T, which also can rescue the immature secretion phenotype induced by the I97L mutation (32).…”
mentioning
confidence: 99%