Novel Primers From Informative Nuclear Loci for Louse Molecular Phylogenetics (Insecta: Phthiraptera)

Sweet, Andrew D.; Allen, Julie M.; Johnson, Kevin P.

doi:10.1603/me13218

Cited by 13 publications

(12 citation statements)

References 33 publications

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“…The PCR amplification conditions followed those of Sweet et al . (), with an annealing temperature of 46 °C (except for EF‐1α , for which the annealing temperature was 50 °C). Sequencing reactions were performed using 1 μL of BigDye and were then submitted for sequencing on an ABI 3730xl capillary machine at the University of Illinois Keck Center for Comparative and Functional Genomics.…”

Section: Methodsmentioning

confidence: 99%

“…After extraction, polymerase chain reaction (PCR) amplification was performed in 50-μL reaction volumes to amplify four genes: one mitochondrial protein-coding gene, cytochrome oxidase I (COI); and three nuclear protein-coding genes: elongation factor-1α (EF-1α), hypothetical protein EOG9XHC5 (hyp), and transmembrane emp24 domain-containing protein 6 precursor (TMEDE6). Primers L6625 and H7005 (Hafner et al, 1994) were used for COI, primers Ef1-For3 and Ef1-Cho10 (Danforth & Ji, 1998) were used for EF-1α, primers BR50-181L and BR50-621R (Sweet, Allen, & Johnson, 2014) were used for hyp, and primers BR69-190F and BR69-432R (Sweet et al, 2014) were used for TMEDE6. The PCR amplification conditions followed those of Sweet et al (2014), with an annealing temperature of 46°C (except for EF-1α, for which the annealing temperature was 50°C).…”

Section: Sequencingmentioning

confidence: 99%

“…Primers L6625 and H7005 (Hafner et al, 1994) were used for COI, primers Ef1-For3 and Ef1-Cho10 (Danforth & Ji, 1998) were used for EF-1α, primers BR50-181L and BR50-621R (Sweet, Allen, & Johnson, 2014) were used for hyp, and primers BR69-190F and BR69-432R (Sweet et al, 2014) were used for TMEDE6. The PCR amplification conditions followed those of Sweet et al (2014), with an annealing temperature of 46°C (except for EF-1α, for which the annealing temperature was 50°C). Sequencing reactions were performed using 1 μL of BigDye and were then submitted for sequencing on an ABI 3730xl capillary machine at the University of Illinois Keck Center for Comparative and Functional Genomics.…”

Section: Sequencingmentioning

confidence: 99%

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Independent origins of the feather lice (Insecta:Degeeriella) of raptors

Catanach

Johnson

2015

Biol J Linn Soc Lond

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Although diurnal birds of prey have historically been placed in a single order due to a number of morphological characters, recent molecular phylogenies have suggested that this is a case of convergence rather than homology, with hawks (Accipitridae) and falcons (Falconidae) forming two distantly related groups within birds. The feather lice of birds have often been used as a model for comparing host and parasite phylogenies, and in some cases there is significant congruence between the two. Thus, studying the phylogeny of the lice of diurnal raptors may be of particular interest with respect to the independent evolution of hawks vs. falcons. Using one mitochondrial gene and three nuclear genes, we inferred a phylogeny for the feather louse genus Degeeriella (which are all obligate raptor ectoparasites) and related genera. This phylogeny indicated that Degeeriella is polyphyletic, with lice from falcons vs. hawks forming two distinct clades. Falcon lice were sister to lice from African woodpeckers, whereas Capraiella, a genus of lice from rollers lice, was embedded within Degeeriella from hawks. This phylogeny showed significant geographical structure, with host geography playing a larger role than host taxonomy in explaining louse phylogeny, particularly within clades of closely related lice. However, the louse phylogeny does reflect host phylogeny at a broad scale; for example, lice from the hawk genus Accipiter form a distinct clade. © 2015 The Linnean Society of London, Biological Journal of the Linnean Society, 2015, 114, 837–847.

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Section: Methodsmentioning

confidence: 99%

Section: Sequencingmentioning

confidence: 99%

Section: Sequencingmentioning

confidence: 99%

See 1 more Smart Citation

Independent origins of the feather lice (Insecta:Degeeriella) of raptors

Catanach

Johnson

2015

Biol J Linn Soc Lond

Self Cite

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“…The nuclear elongation factor 1 (EF-1 ) gene was amplified using the primers For3 and CHO10 for EF-1 (Danforth & Ji, 1998). For a protein of unknown function [hyp; see Sweet et al (2014)], the primers BR50-181L and BR50-621R were used (Sweet et al, 2014). The success of the PCR was confirmed using gel electrophoresis with a 1% agarose gel and GelGreen (Biotium, Inc., Hayward, CA, U.S.A.).…”

Section: Data Collectionmentioning

confidence: 99%

Patterns of cryptic host specificity in duck lice based on molecular data

Escalante

Sweet

McCracken

et al. 2016

Medical Vet Entomology

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Documenting patterns of host specificity in parasites relies on the adequate definition of parasite species. In many cases, parasites have simplified morphology, making species delimitation based on traditional morphological characters difficult. Molecular data can help in assessing whether widespread parasites harbour cryptic species and, alternatively, in guiding further taxonomic revision in cases in which there is morphological variation. The duck louse genus Anaticola (Phthiraptera: Philopteridae), based on current taxonomy, contains both host-specific and widespread species. Mitochondrial and nuclear DNA sequences of samples from this genus were used to document patterns of host specificity. The comparison of these patterns with morphological variations in Anaticola revealed a general correspondence between the groups identified by DNA sequences and morphology, respectively. These results suggest that a more thorough taxonomic review of this genus is needed. In general, the groups identified on the basis of molecular data were associated with particular groups of waterfowl (e.g. dabbling ducks, sea ducks, geese) or specific biogeographic regions (e.g. North America, South America, Australia, Eurasia).

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“…These primers, reliably amplifying louse DNA samples of varying quantity and quality, were selected to provide a gross picture of population structure across the whole sample set. For better understanding of the relationships between main mtDNA lineages of lice, a longer fragment of COI (1027bp), together with three nuclear genes VATP21 (304bp), hyp (380bp) and TMEDE6 (215bp), were obtained for selected specimens of P. serrata (n=25), using COI primers LCO1490 and H7005 (Folmer et al 1994) and nuclear primers published by Sweet et al (2014). Description of the PCR reactions, thermal cycling conditions and sequencing are provided in Document S1 (Supporting information).…”

Section: Dna Sequencing and Population Analysismentioning

confidence: 99%

Flexibility of co-evolutionary patterns in ectoparasite populations: genetic structure and diversity inApodemusmice and their lice

Martinů

Hypša

Štefka

2016

Preprint

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Abstract:Host-parasite co-evolution belongs among the major processes governing evolution of biodiversity on the global scale. Numerous studies performed at inter-specific level revealed variety of patterns from strict co-speciation to lack of co-divergence and frequent host-switching, even in species tightly linked to their hosts. To explain these observations and formulate ecological hypotheses, we need to acquire better understanding to parasites' population genetics and dynamics, and their main determinants. Here, we analyse the impact of co-evolutionary processes on genetic diversity and structure of parasite populations, using a model composed of the louse Polyplax serrata and its hosts, mice of the genus Apodemus, collected from several dozens of localities across Europe. We use mitochondrial DNA sequences and microsatellite data to describe the level of genealogical congruence between hosts and parasites and to assess genetic diversity of the populations. We also explore links between the genetic assignment of the parasite and its host affiliation, and test the prediction that populations of the parasite possessing narrower host specificity show deeper pattern of population structure and lower level of genetic diversity as a result of limited dispersal and smaller effective population size. We demonstrate an overall complexity of the co-evolutionary processes and their variability even among closely related lineages of the parasites. In the analysis of several sympatric parasite populations, we find strong evidence for the link between the width of host specificity and genetic diversity of parasites.

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Novel Primers From Informative Nuclear Loci for Louse Molecular Phylogenetics (Insecta: Phthiraptera)

Cited by 13 publications

References 33 publications

Independent origins of the feather lice (Insecta:Degeeriella) of raptors

Independent origins of the feather lice (Insecta:Degeeriella) of raptors

Patterns of cryptic host specificity in duck lice based on molecular data

Flexibility of co-evolutionary patterns in ectoparasite populations: genetic structure and diversity inApodemusmice and their lice

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