Novel probiotic Enterococcus faecium IS-27526 supplementation increased total salivary sIgA level and bodyweight of pre-school children: A pilot study

Abstract

Enterococcus faecium IS-27526 is a novel probiotic isolated from dadih, an Indonesian traditional fermented buffalo milk. A 90 days randomized double-blind placebo-controlled study of pre-post trial was conducted in pre-school children with two groups, placebo and probiotic group. Ultra High Temperature low fat milk was used as a carrier in each group. The aims of this study were to investigate the effect of E. faecium IS-27526 in milk on humoral immune response and on bodyweight of pre-school children. Total serum IgA and total salivary sIgA were measured by sandwich ELISA. The bodyweight of young children was measured. The results showed that total serum IgA did not significantly increase in the probiotic group compared with the placebo group. Total salivary sIgA level and the bodyweight significantly increased (p < 0.05) in probiotic groups compared to the placebo. Changes of total salivary sIgA level were significantly higher in underweight children supplemented with probiotic. Weight gain was observed significantly in children with normal bodyweight supplemented with probiotic. Neither mortality nor weight loss was recorded throughout the study. Taken together, novel probiotic E. faecium IS-27526 has significant positive effects on humoral immune response, salivary sIgA, in underweight pre-school children, and on weight gain of pre-school children.

A review: Health promoting lactic acid bacteria in traditional Indonesian fermented foods

“…Supplementation with the probiotic and zinc resulted in a significantly increased humoral immune response, as well as improved zinc status. The effects of probiotics on the humoral immune response was suggested to be the result of colonization and adhesion to epithelial cells, and production of SIgA production induced by the cell wall component of probiotics, such as lipoteichoic acids and peptidoglycan [69]. Both strong immune responses and gut integrity play important roles in the repair of intestinal brush border damage as a result of the balance of microbiota.…”

“…Two potential probiotics originating from dadih have gone through a clinical study in humans [69,70]. Surono et al [69] evaluated the effect of E. faecium IS-27526 in milk on humoral immune response and on bodyweight of young children aged between 15 and 54 months. A 90 days randomized, double-blind, placebo-controlled study was conducted with two groups of young children, placebo and probiotic group.…”

Isolasi Bakteri Asam Laktat Berpotensi Probiotik Pada Dangke, Makanan Tradisional dari Susu Kerbau di Curio Kabupaten Enrekang

“…Penelitian tentang isolasi bakteri berpotensi probiotik telah banyak dilakukakan diantaranya dari feces manusia (Tinrat et al, 2011), tinja bayi (Chiang et al, 2012), acar kyoto (Nonaka et al, 2008), kefir , kimchi (Won et al, 2011), dan sake (Kawamoto et al, 2011). Beberapa penelitian yang lain melakukan isolasi bakteri dari susu dan olahan susu, diantaranya dari Air Susu Ibu (Djide et al, 2008), susu sapi (Baruzzi et al, 2011), susu kerbau (Duary et al, 2011), susu kuda (Shi et al, 2012), susu fermentasi (Vizoso et al, 2007), dadih (Surono et al, 2011), dan dangke (Razak et al, 2009).…”

Microencapsulation increases survival of the probioticLactobacillus plantarumIS-10506, but notEnterococcus faeciumIS-27526 in a dynamic, computer-controlledin vitromodel of the upper gastrointestinal tract

“…) and in human studies have demonstrated enhancement of humoural immune response, and nutrient absorption in several studies (Surono et al . , ).…”