Novel Probiotic Mechanisms of the Oral Bacterium Streptococcus sp. A12 as Explored with Functional Genomics

Abstract

Health-associated biofilms in the oral cavity are composed of a diverse group of microbial species that can foster an environment that is less favorable for the outgrowth of dental caries pathogens, like Streptococcus mutans. A novel oral bacterium, designated Streptococcus A12, was previously isolated from supragingival dental plaque of a caries-free individual and was shown to interfere potently with the growth and virulence properties of S. mutans. In this study, we applied functional genomics to begin to identify molecular mechanisms used by A12 to antagonize, and to resist the antagonistic factors of, S. mutans. Using bioinformatics, genes that could encode factors that enhance the ability of A12 to compete with S. mutans were identified. Selected genes, designated potential competitive factors (pcf), were deleted. Certain mutant derivatives showed a reduced capacity to compete with S. mutans compared to that of the parental strain. The A12 pcfO mutant lost the ability to inhibit comX-inducing peptide (XIP) signaling by S. mutans, while mutants with changes in the pcfFEG locus were impaired in sensing of, and were more sensitive to, the lantibiotic nisin. Loss of PcfV, annotated as a colicin V biosynthetic protein, resulted in diminished antagonism of S. mutans. Collectively, the data provide new insights into the complexities and variety of factors that affect biofilm ecology and virulence. Continued exploration of the genomic and physiological factors that distinguish commensals from truly beneficial members of the oral microbiota will lead to a better understanding of the microbiome and new approaches to promote oral health. IMPORTANCE Advances in defining the composition of health-associated biofilms have highlighted the important role of beneficial species in maintaining health. Comparatively little, however, has been done to address the genomic and physiological bases underlying the probiotic mechanisms of beneficial commensals. In this study, we explored the ability of a novel oral bacterial isolate, Streptococcus A12, to compete with the dental pathogen Streptococcus mutans using various gene products with diverse functions. A12 displayed enhanced competitiveness by (i) disrupting intercellular communication pathways of S. mutans, (ii) sensing and resisting antimicrobial peptides, and (iii) producing factors involved in the production of a putative antimicrobial compound. Research on the probiotic mechanisms employed by Streptococcus A12 is providing essential insights into how beneficial bacteria may help maintain oral health, which will aid in the development of biomarkers and therapeutics that can improve the practice of clinical dentistry.

Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans

“…Recently our group reported that part of the arsenal deployed by S. sp. A12, a health-associated commensal with probiotic properties, to compete with the caries pathogen S. mutans includes its ability to degrade peptide cell-cell signaling molecules [18,19]. In particular, incubation of synthetic CSP or XIP with only culture supernates alone of S. sp.…”

“…In particular, incubation of synthetic CSP or XIP with only culture supernates alone of S. sp. A12 abolishes the peptides' activity via Sgc or PcfO, respectively [18,19]. We set out in this study to explore how ComRS signaling in S. mutans is altered during direct growth with these competitor species, conditions that should more closely mimic oral biofilms.…”

“…Ten different low-passage clinical isolates of S. gordonii, ten isolates of S. sanguinis, and five isolates of S. sp. A12related organisms [19] were cocultured with our S. mutans ComRS signaling reporter. The S. sp.…”

“…Recently, it was shown that a novel Streptococcus strain, designated Streptococcus sp. A12, has the ability to degrade the two primary cell–cell signal peptides of S. mutans , competence stimulating peptide (CSP) and com X - inducing peptide (XIP) [ 18 , 19 ], suggesting that inference of cell–cell communication could be an underappreciated antagonistic mechanism that contributes to caries resistance.…”

In Vivo Colonization with Candidate Oral Probiotics Attenuates Streptococcus mutans Colonization and Virulence

“…on December 13, 2020 by guest http://aem.asm.org/ Downloaded from 5 A strategy gaining acceptance to support the health of dental tissues is use of probiotics;…”