Novel Pyrazolo[3,4-&lt;i&gt;b&lt;/i&gt;]pyridine Derivatives: Synthesis, Characterization, Antimicrobial and Antiproliferative Profile

Salem, Marwa S.; Ali, Mohamed A.

doi:10.1248/bpb.b15-00586

Cited by 67 publications

(37 citation statements)

References 43 publications

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“…Following overnight incubation at 37°C for 24 h (bacterial strains) or at 25°C for 4-10 days (fungal strains), the tubes are examined for the presence of visible microbial growth by turbidity. The lowest concentration of the antimicrobial agent where the growth was completely inhibited (no turbidity) represents the MIC (Salem and Ali 2016). The precision of broth macrodilution was considered to be plus/minus one twofold dilution concentration, due to the manual preparation of the dilutions (CLSI 2018b).…”

Section: Broth Macrodilutionmentioning

confidence: 99%

Antimicrobial susceptibility testing: currently used methods and devices and the near future in clinical practice

Benková

Soukup

Marek

2020

J. Appl. Microbiol.

137

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Summary Rapid identification of pathogen and its resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment are essential for correct patient outcomes. Conventional detection methods of bacterial resistance, such as disc diffusion, broth microdilution and automated instruments, are constantly widely used and primarily standardized. Nevertheless, the results cannot be obtained earlier than 48 h after receiving a sample, which may lead to prolonged use or overuse of broad‐spectrum antibiotics. Hence, there is a drive to develop and introduce novel, faster, standardized, sensitive and specific methods with reliable results into routine microbiological laboratory practice. Recently developed matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) has been introduced in recent years into laboratory practice, and methods based on microfluidics and microdroplets might be introduced in the near future. This review is focused on the methods and instruments in use both currently and in the foreseeable future, applicable to determine antimicrobial efficacy in clinical microbiology laboratories.

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Section: Broth Macrodilutionmentioning

confidence: 99%

Antimicrobial susceptibility testing: currently used methods and devices and the near future in clinical practice

Benková

Soukup

Marek

2020

J. Appl. Microbiol.

137

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“…In continuation of our previous work, the present work aims at utilization of the reactivity of 2‐thioxo‐1,2‐dihydrobenzo[d][1,3]thiazin‐4‐one ( 1 ) toward different electrophilic and nucleophilic reagents to design and construct new fused and nonfused heterocyclic systems and evaluate them for antitumor activity.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and cytotoxic activity against human tumor cells of heterocyclic systems derived from 2‐thioxo‐1,2‐dihydro‐4H‐3,1‐benzothazin‐4‐one

Khlosy

Salem

Ali

et al. 2019

Journal of Heterocyclic Chem

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The title compound was prepared and converted to 2-hydrazinyl-1,2-dihydro-4H-benzo[d] [1,3]thiazin-4-one which was utilized to synthesize fused heterocyclic systems, namely benzotriazolothiazinone derivatives, as well as, nonfused heterocyclic systems such as pyrazolyl-benzothiazinones, benzothiazinylpyridazine and imidazolylbenzothiazinone derivatives via reaction with formamide, acetic acid, ethyl cyanoacetate, maleic anhydride and benzaldehyde followed by treatment with glycine, respectively. All compounds have been structurally characterized by means of IR, MS, and 1 H-NMR spectra. The synthesized compounds were evaluated in vitro for their antiproliferative activity against HePG-2 and MCF-7 cell lines. 2H-Benzo[d][1,3]thiazine-2,4 (1H)-dithione and 2-thioxo-1,2-dihydro-4H-benzo[d] [1,3]thiazin-4-one were the most potent against the two cancer cells compared to that of the reference compound doxorubicin. Most of the synthesized compounds also exhibited good cytotoxic activity.

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“…Pyrazolopyridines represent a well-known organic scaffold in various bioactive derivatives and have various pharmacological properties. These compounds exhibit significant biological properties, including anti-bacterial [1,2], anti-microbial [3,4], anti-fungal [5], anti-tumor [6], anti-virus [7], anti-Leishmania [8], HIF 1-a-prolyl hydroxylase inhibitors [9], B-Raf V600E3 inhibitors [10], protein kinase inhibitors [11], PDE4B inhibitors [12], dopaminergic properties [13] and cancer cell lines growth inhabitation activities [14]. The main problems for the synthesis of pyrazolopyridine compounds are long reaction times, utilization of non-reusable and toxic catalyst and use of particular conditions.…”

Section: Introductionmentioning

confidence: 99%

KCC-1-NH₂-DPA: an efficient heterogeneous recyclable nanocomposite for the catalytic synthesis of tetrahydrodipyrazolopyridines as a well-known organic scaffold in various bioactive derivatives

2019

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Novel Pyrazolo[3,4-<i>b</i>]pyridine Derivatives: Synthesis, Characterization, Antimicrobial and Antiproliferative Profile

Cited by 67 publications

References 43 publications

Antimicrobial susceptibility testing: currently used methods and devices and the near future in clinical practice

Antimicrobial susceptibility testing: currently used methods and devices and the near future in clinical practice

Synthesis and cytotoxic activity against human tumor cells of heterocyclic systems derived from 2‐thioxo‐1,2‐dihydro‐4H‐3,1‐benzothazin‐4‐one

KCC-1-NH₂-DPA: an efficient heterogeneous recyclable nanocomposite for the catalytic synthesis of tetrahydrodipyrazolopyridines as a well-known organic scaffold in various bioactive derivatives

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Novel Pyrazolo[3,4-<i>b</i>]pyridine Derivatives: Synthesis, Characterization, Antimicrobial and Antiproliferative Profile

Cited by 67 publications

References 43 publications

Antimicrobial susceptibility testing: currently used methods and devices and the near future in clinical practice

Antimicrobial susceptibility testing: currently used methods and devices and the near future in clinical practice

Synthesis and cytotoxic activity against human tumor cells of heterocyclic systems derived from 2‐thioxo‐1,2‐dihydro‐4H‐3,1‐benzothazin‐4‐one

KCC-1-NH2-DPA: an efficient heterogeneous recyclable nanocomposite for the catalytic synthesis of tetrahydrodipyrazolopyridines as a well-known organic scaffold in various bioactive derivatives

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KCC-1-NH₂-DPA: an efficient heterogeneous recyclable nanocomposite for the catalytic synthesis of tetrahydrodipyrazolopyridines as a well-known organic scaffold in various bioactive derivatives