Novel Pyrimidine Tagged Silver Nanoparticle Based Fluorescent Immunoassay for the Detection of Pseudomonas aeruginosa

Abstract

A simple pyrimidine-based fluorescent probe (R)-4-(anthracen-9-yl)-6- (naphthalen-1-yl)-1,6-dihydropyrimidine-2-amine (ANDPA) was synthesized through the greener one pot reaction and characterized by IR, NMR, and ESI-Mass. Glucose stabilized silver nanoparticles (Glu-AgNPs) were also synthesized and characterized using UV, IR, XRD, SEM, and TEM. When ANDPA was tagged with Glu-AgNPs, the fluorescent intensity of ANDPA decreased drastically. When the monoclonal antibody (Ab) [immunoglobulin G (IgG)] of Pseudomonas aeruginosa (PA) was attached with ANDPA/Glu-AgNPs, the original intensity of the probe was recovered with minimal enhancement at 446 nm. On further attachment of PA with ANDPA/Glu-AgNPs/PA, the fluorescence intensity of the probe was enhanced obviously at 446 nm with red shift. This phenomenon was further supported by SEM and TEM. The linear range of detection is from 8 to 10 CFU/mL, and LOD is 1.5 CFU/mL. The immunosensor was successfully demonstrated to detect Pseudomonas aeruginosa in water, soil, and food products like milk, sugar cane, and orange juices.

Designing of New Optical Immunosensors Based on 2-Amino-4-(anthracen-9-yl)-7-hydroxy-4H-chromene-3-carbonitrile for the Detection of Aeromonas hydrophila in the Organs of Oreochromis mossambicus Fingerlings

“…56−58 Therefore, the fluorescent intensity of the immunoassay was increased. 51,59,60 Therefore, based on these UV–vis and emission spectral changes, it is confirmed that two interesting mechanisms such as inhibition of twisted ICT and AIE are existing during the formation of anti-AH–AHC and AH/anti-AH–AHC immunocomplexes, respectively, under optimized conditions (Scheme 1).…”

Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples

“…Recently, efforts have been devoted to the rapid and sensitive detection of P. aeruginosa using various approaches. Different recognition elements are used, including monoclonal antibody ( Ellairaja et al, 2017 ), DNA aptamers ( Zhong, Gao, Chen, & Jia, 2020 ), fluorescent organic nanoparticles ( Kaur, Raj, Kaur, & Singh, 2015 ). These elements enhance the sensitivity for P. aeruginosa detection and reduce the assay time.…”

Nanomaterial enabled sensors for environmental contaminants

“…Most of the detection schemes reported for P. aeruginosa focus on whole pathogen detection [ 142 – 146 ] with the work of Melanie et al [ 139 ], discussed above, on 16s rRNA detection being an outlier. In addition, to oligonucleotide recognition elements [ 139 , 142 – 144 ], antibodies [ 145 , 147 ] and bacteriophages [ 146 ] have also been used for specific detection of P. aeruginosa .…”