Novel Reductant for Determination of Total Plasma Homocysteine

Gilfix, Brian M.; Blank, David; Rosenblatt, David S.

doi:10.1093/clinchem/43.4.687

Cited by 144 publications

(48 citation statements)

References 5 publications

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“…All the samples were assayed for Homocysteine by using High Performance Liquid Chromatography with fluorescent detection technique (HPLC‐fld), as proposed by Ubbink & Vermaak (, ) and later modified by Gilfix et al. () , which has a reported sensitivity of >0.2 μ mol/l. In brief, standard solutions of progressively decreasing molar concentrations (For e.g., 1 M, 0.5 M) were prepared using DL‐Homocysteine standard (Sigma‐Aldrich, St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Effect of non‐surgical periodontal therapy on plasma homocysteine levels in Indian population with chronic periodontitis: a pilot study

Bhardwaj

Prabhuji

Karthikeyan

2015

J Clinic Periodontology

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Section: Methodsmentioning

confidence: 99%

Effect of non‐surgical periodontal therapy on plasma homocysteine levels in Indian population with chronic periodontitis: a pilot study

Bhardwaj

Prabhuji

Karthikeyan

2015

J Clinic Periodontology

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“…17 tHcy was measured by high-performance liquid chromatography using postcolumn fluorescence detection. 18 MMA was determined on an Agilent liquid chromatography/mass spectrometry system (Agilent Technologies, Santa Clara, CA).…”

Section: Serum B12 Status Measurementsmentioning

confidence: 99%

Vitamin B12 and Homocysteine Levels Predict Different Outcomes in Early Parkinson's Disease

et al. 2018

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In this study of early PD, low B12 status was common. Low B12 at baseline predicted greater worsening of mobility whereas elevated homocysteine predicted greater cognitive decline. Given that low B12 and elevated homocysteine can improve with vitamin supplementation, future studies should test whether prevention or early correction of these nutritionally modifiable conditions slows development of disability. © 2018 International Parkinson and Movement Disorder Society.

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“…Total plasma homocysteine and pyridoxal-5 0 -phosphate (PLP), indicating the vitamin B6 status, were determined by high-performance liquid chromatography with postcolumn fluorescence detection. 17,18 Red blood cell (RBC) and plasma folate and plasma vitamin B12 were determined with radioassays (SimulTRAC; MP Biomedicals, Orangeburg, NY). The interassay coefficients of variation of blinded duplicate control samples for each of the assays were as follows: plasma homocysteine, 6.5%; RBC folate, 10.2%; plasma folate, 4.8%; plasma PLP, 4.8%; and plasma vitamin B12, 6.2%.…”

Section: Focm Biomarkers and Covariatesmentioning

confidence: 99%

Folate‐mediated one‐carbon metabolism genes and interactions with nutritional factors on colorectal cancer risk: Women's Health Initiative Observational Study

Cheng

Makar

Neuhouser

et al. 2015

Cancer

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Background Investigations of folate-mediated one-carbon metabolism (FOCM) genes and gene-nutrient interactions in relation to colorectal cancer (CRC) risk are limited to candidate polymorphisms and dietary folate. We comprehensively investigated associations between genetic variants in FOCM and CRC risk, and whether FOCM nutrient status modified these associations. Methods We genotyped 288 candidate and tagging single nucleotide polymorphisms (SNPs) in 30 FOCM genes among 821 incident CRC case-control matched pairs in the Women’s Health Initiative Observational Study cohort. FOCM biomarkers (red blood cell [RBC] folate, plasma folate, pyridoxal-5’-phosphate [PLP], vitamin B12, and homocysteine) and self-reported alcohol consumption were measured at baseline. Conditional logistic regression was implemented; effect modification was examined based on known enzyme-nutrient relationships. Results We observed statistically significant associations between CRC risk and functionally defined candidate SNPs of MTHFD1 (K134R), MTRR (P450R), and PRDM2 (S450N), and a literature candidate SNP of TYMS (g.676789A>T) (nominal P<0.05). In addition we noted suggestive associations for tagSNPs in CBS, DHFR, DNMT3B, MAT1A, MTHFD1, and MTRR (nominal P<0.05; non-significant adjusted P). Significant interactions between nutrient biomarkers and candidate polymorphisms were observed for (i) plasma/RBC folate and FOLH1, PON1, TCN2, DNMT1, and DNMT3B; (ii) plasma PLP and TYMS TS3; (iii) plasma B12 and BHMT2; (iv) homocysteine and MTHFR and AARS. Conclusions Genetic variants in FOCM genes are associated with CRC risk among postmenopausal women. FOCM nutrients continue to emerge as effect modifiers of genetic influences on CRC risk.

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Novel Reductant for Determination of Total Plasma Homocysteine

Cited by 144 publications

References 5 publications

Effect of non‐surgical periodontal therapy on plasma homocysteine levels in Indian population with chronic periodontitis: a pilot study

Effect of non‐surgical periodontal therapy on plasma homocysteine levels in Indian population with chronic periodontitis: a pilot study

Vitamin B12 and Homocysteine Levels Predict Different Outcomes in Early Parkinson's Disease

Folate‐mediated one‐carbon metabolism genes and interactions with nutritional factors on colorectal cancer risk: Women's Health Initiative Observational Study

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