Novel regulation of transcription initiation of the peptide IX gene of adenovirus 2.

Matsui, T

doi:10.1128/mcb.9.10.4265

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…We have not detected any requirements for DNA sequences upstream of the pIX SP1 site or downstream of the pIX TATA box that are important to pIX gene expression. Our studies support those of Matsui (21), which showed that sequences between nt -70 and -45 as well as the TATA box were important to pIX gene expression in transient expression assays, although the identity of the SP1 site was not recognized in that previous work. However, we have not found any sequences internal to the pIX gene that affect pIX gene expression in vivo.…”

Section: Discussionsupporting

confidence: 90%

Promoter of the adenovirus polypeptide IX gene: similarity to E1B and inactivation by substitution of the simian virus 40 TATA element

Babiss

Vales

1991

J Virol

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The promoter of the adenovirus polypeptide IX (pIX) gene consists of an SP1 binding site and a TATA box and is remarkably similar to the promoter of the E1B gene in which it is nested. Plasmid constructs containing the pIX gene with deletions in the SP1 or TATA sites were defective in pIX mRNA production in transient expression assays. These results were confirmed with analogous virus constructs. An oligonucleotide containing sequences within the pIX promoter region spanning the SP1 and TATA sites but not including the sequences downstream of the TATA box is sufficient to direct mRNA synthesis at +90 nucleotides within the pIX gene. While the simian virus 40 (SV40) early promoter is capable of directing pIX mRNA synthesis from the SV40 cap sites, substitution of the pIX TATA box with the SV40 TATA box results in barely detectable levels of pIX mRNA. These results will be discussed with respect to exchangeability of promoter elements and the possible role of the viral E1B 21-kDa protein in potentiating or stabilizing transcription factor TFIID binding to the pIX TATA element.

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Section: Discussionsupporting

confidence: 90%

Promoter of the adenovirus polypeptide IX gene: similarity to E1B and inactivation by substitution of the simian virus 40 TATA element

Babiss

Vales

1991

J Virol

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“…Once again, the peak with the highest propensity value is at À70 bp relative to the TSS. A TATA box has been reported at position À30 in this promoter, to which TBP binds and directs transcription (53). There is also some other, unidentified activating upstream element from À45 to À70 bp relative to the TSS.…”

Section: Protein IX Promotermentioning

confidence: 90%

Profiling the Thermodynamic Softness of Adenoviral Promoters

Choi

Rapti

Gelev

et al. 2008

Biophysical Journal

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We showed previously that anharmonic DNA dynamical features correlate with transcriptional activity in selected viral promoters, and hypothesized that areas of DNA softness may represent loci of functional significance. The nine known promoters from human adenovirus type 5 were analyzed for inherent DNA softness using the Peyrard-Bishop-Dauxois model and a statistical mechanics approach, using a transfer integral operator. We found a loosely defined pattern of softness peaks distributed both upstream and downstream of the transcriptional start sites, and that early transcriptional regions tended to be softer than late promoter regions. When reported transcription factor binding sites were superimposed on our calculated softness profiles, we observed a close correspondence in many cases, which suggests that DNA duplex breathing dynamics may play a role in protein recognition of specific nucleotide sequences and protein-DNA binding. These results suggest that genetic information is stored not only in explicit codon sequences, but also may be encoded into local dynamic and structural features, and that it may be possible to access this obscured information using DNA dynamics calculations.

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“…The pIX and E21 genes, like the Elb gene, have an Spl-binding site consensus sequence (GC box; 29) located within 30 bp 5' of a TATA box (50). Each GC box mediates transcription (14,31), and at least the E21 site (14), like the Elb site (63), binds purified Spl (14,63). No other adenovirus promoters have this arrangement of adjacent GC and TATA boxes, which may constitute the target of a shared mechanism for sequential transcription activation.…”

Section: Discussionmentioning

confidence: 99%

cis-dominant defect in activation of adenovirus type 5 E1b early RNA synthesis

Parks

Spector

1990

J Virol

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A cis-dominant mutation in the adjacent Ela gene disrupted the accumulation of adenovirus type 5 Elb mRNA during the early phase of infection. Steady-state levels of cytoplasmic and nuclear Elb RNAs in cells infected with d1312, a strain that lacks the Ela TATA box, cap site, and much of the coding sequence, were reduced 5to 10-fold even when the Ela activator was provided in trans. The strain was defective for early Elb RNA synthesis but not for Elb RNA made late or during prolonged incubation in the presence of an inhibitor of DNA replication. The defect in Elb RNA synthesis could not be attributed to the Ela promoter sequences missing in d1312 DNA. If the Ela protein-coding region contains cis-acting regulatory sequences, they are not part of the previously mapped Elb transcriptional control region and may represent additional regulatory elements that ensure prompt and efficient Elb expression during the early phase of infection.

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Novel regulation of transcription initiation of the peptide IX gene of adenovirus 2.

Cited by 5 publications

References 25 publications

Promoter of the adenovirus polypeptide IX gene: similarity to E1B and inactivation by substitution of the simian virus 40 TATA element

Promoter of the adenovirus polypeptide IX gene: similarity to E1B and inactivation by substitution of the simian virus 40 TATA element

Profiling the Thermodynamic Softness of Adenoviral Promoters

cis-dominant defect in activation of adenovirus type 5 E1b early RNA synthesis

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