Novel regulation targets of the metal-response BasS–BasR two-component system of Escherichia coli

Ogasawara, Hiroshi; Shinohara, Shota; Yamamoto, Kaneyoshi; Ishihama, Akira

doi:10.1099/mic.0.057745-0

Cited by 56 publications

(52 citation statements)

References 51 publications

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“…To test this, we knocked out known ironresponsive transcriptional factors that have also been shown to affect biofilm formation. Our candidates included the global iron regulator, Fur (11,12), the iron-sulfur cluster regulator, IscR (9), the ferric iron-sensing two-component system, BasSR (36), and the small RNA, RyhB (10). All of the mutants still formed rugose biofilms in response to iron (Fig.…”

Section: Curliated Bacteria Are Localized To the Air-biofilm Interfacmentioning

confidence: 99%

Iron induces bimodal population development by Escherichia coli

DePas¹,

Hufnagel

Lee

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

110

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Bacterial biofilm formation is a complex developmental process involving cellular differentiation and the formation of intricate 3D structures. Here we demonstrate that exposure to ferric chloride triggers rugose biofilm formation by the uropathogenic Escherichia coli strain UTI89 and by enteric bacteria Citrobacter koseri and Salmonella enterica serovar typhimurium. Two unique and separable cellular populations emerge in iron-triggered, rugose biofilms. Bacteria at the air-biofilm interface express high levels of the biofilm regulator csgD, the cellulose activator adrA, and the curli subunit operon csgBAC. Bacteria in the interior of rugose biofilms express low levels of csgD and undetectable levels of matrix components curli and cellulose. Iron activation of rugose biofilms is linked to oxidative stress. Superoxide generation, either through addition of phenazine methosulfate or by deletion of sodA and sodB, stimulates rugose biofilm formation in the absence of high iron. Additionally, overexpression of Mn-superoxide dismutase, which can mitigate iron-derived reactive oxygen stress, decreases biofilm formation in a WT strain upon iron exposure. Not only does reactive oxygen stress promote rugose biofilm formation, but bacteria in the rugose biofilms display increased resistance to H 2 O 2 toxicity. Altogether, we demonstrate that iron and superoxide stress trigger rugose biofilm formation in UTI89. Rugose biofilm development involves the elaboration of two distinct bacterial populations and increased resistance to oxidative stress.functional amyloid | biofilm matrix | wrinkled colony

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Section: Curliated Bacteria Are Localized To the Air-biofilm Interfacmentioning

confidence: 99%

Iron induces bimodal population development by Escherichia coli

DePas¹,

Hufnagel

Lee

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

110

View full text Add to dashboard Cite

show abstract

“…Alternatively, a recent study shows in E. coli that pmrAB (referred to as basSR in that study) controls a much wider range of genes than previously published, including genes responsible for stress response regulation and metabolism. 7 In light of these findings, the decreased stress response may pair with antimicrobial peptide resistance to synergistically cause the observed attenuation in an environment where both challenges are present.…”

Section: Discussionmentioning

confidence: 99%

“…5,6 These three transcription factors play a key role in the persistence of E. coli in a murine kidney infection model, where deletion of all three genes significantly decreases the bacterial load in the kidney after three days of successful colonization. 5 Previous microarray data show that expression levels of genes associated with the pmrAB regulon 6,7 were decreased in a triple mutant lacking the transcription factors MarA, Rob, and SoxS compared with its parent strain, when extracted from a murine pyelonephritis infection model. 6 The pmrAB operon encodes a two-component regulatory system present in many bacterial species, 8,9 which mediates a wide range of functions, most notably modification of the lipid A moiety of lipopolysaccharide (LPS).…”

Section: Introductionmentioning

confidence: 99%

The contribution of PmrAB to the virulence of a clinical isolate ofEscherichia coli

2013

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“…Using Genomic SELEX screening, we have identified a total of at least 38 BasR-binding sites of phosphorylated BasR on the E. coli genome, and predicted a number of regulation target genes involved in the modulation of membrane structure and functions and the activation of stress response genes, including the master regulator CsgD of biofilm formation. 91) BasR specifically binds to the DNA region including a direct repeat of a TTAAnnTT sequence. 91) 4.9.…”

Section: Iron-sensing Regulatorsmentioning

confidence: 99%

“…91) BasR specifically binds to the DNA region including a direct repeat of a TTAAnnTT sequence. 91) 4.9. Molybdate-sensing transcription factor ModE E. coli growing under anaerobic conditions produces several molybdoenzymes containing a molybdenum cofactor in their active site to catalyze various redox reactions, such as formate hydrogenlyase and nitrate reductase.…”

Section: Iron-sensing Regulatorsmentioning

confidence: 99%