Novel <scp>CCK</scp>‐dependent vasorelaxing dipeptide, <scp>A</scp>rg‐<scp>P</scp>he, decreases blood pressure and food intake in rodents

Kagebayashi, Tomomi; Kontani, Noriyasu; Yamada, Yuko; Maekawa, Takafumi; Arai, Tomomi; Kino, Kuniki; Ohinata, Kousaku

doi:10.1002/mnfr.201200168

Cited by 43 publications

(54 citation statements)

References 42 publications

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“…The meal transit rate in the small intestine can be associated with the food intake and fat absorption and is often used to evaluate the suppressive effects on food intake, appetite, and digestion (14,26). However, like the results of intestinal fat absorption, the meal transit was not suppressed by a single administration of the EW or EWH (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Egg White Hydrolysate Can Be a Low-Allergenic Food Material to Suppress Ectopic Fat Accumulation in Rats Fed an Equicaloric Diet

Ochiai

Misaki

Takeuchi

et al. 2017

J Nutr Sci Vitaminol

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Summary Egg white (EW) is known as a nutritional protein but can induce allergic reactions in humans. We investigated the dietary effects of EW and its hydrolysate (EWH), which contains less allergen, on body fat accumulation in Wistar rats fed an equicaloric high-fat and high-sucrose diet for 8 wk (Exp A). The pair-feeding of EW and equicaloric-feeding of EWH increased fecal fat excretion and suppressed lipid accumulation in the liver and muscles but not in the abdominal adipose tissues, carcass, or total body. Dietary EWH also suppressed the serum glucose level and alkaline phosphatase activity. Further, we showed a higher dispersibility of EW and EWH in physicochemical assay (Exp B). Next, we investigated the suppressive effects of a single administration of EW and EWH on lipid-induced hypertriglyceridemia and small intestinal meal transit in ddY mice (Exp C). However, a single administration of EW or EWH did not suppress the lipid-induced hypertriglyceridemia nor did it delay the rate of small intestinal transit. These findings indicated that dietary EW and EWH reduce hepatic and muscular (ectopic) fat accumulation mainly by suppressing fat absorption and supplying fat to the liver and muscles. Therefore, the low-allergenic EWH can be effective for the prevention of high-fat-diet-induced obesity.

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Section: Resultsmentioning

confidence: 99%

“…The small-intestinal transit test was carried out according to a previously reported method (14). Half of the mice in each group were fasted for 12 h with free access to water.…”

Section: Exp C: Effect Of a Single Administration Of Ew And Ewh On LImentioning

confidence: 99%

Egg White Hydrolysate Can Be a Low-Allergenic Food Material to Suppress Ectopic Fat Accumulation in Rats Fed an Equicaloric Diet

Ochiai

Misaki

Takeuchi

et al. 2017

J Nutr Sci Vitaminol

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“…[92,93]. Interestingly, the dipeptide RF sequence, which was reported to stimulate CCK release is found in this peptide [94].…”

Section: D) Soy Proteinsmentioning

confidence: 96%

“…The peptide was named -casein exorphin since it corresponds to the s1-casein (90)(91)(92)(93)(94)(95)(96). Its fragments peptides RYLGY and YLGYE also showed weaker activity than the hexapeptide.…”

Section: S1-caseinmentioning

confidence: 99%

Bioactive peptides derived from natural proteins with respect to diversity of their receptors and physiological effects

Yoshikawa

2015

Peptides

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We have found various bioactive peptides derived from animal and plant proteins, which interact with receptors for endogenous bioactive peptides such as opioids, neurotensin, complements C3a and C5a, oxytocin, and formyl peptides etc. Among them, rubiscolin, a δ opioid peptide derived from plant RuBisCO, showed memory-consolidating, anxiolytic-like, and food intake-modulating effects. Soymorphin, a μ opioid peptide derived from β-conglycinin showed anxiolytic-like, anorexigenic, hypoglycemic, and hypotriglyceridemic effects. β-Lactotensin derived from β-lactoglobulin, the first natural ligand for the NTS2 receptor, showed memory-consolidating, anxiolytic-like, and hypocholesterolemic effects. Weak agonist peptides for the complements C3a and C5a receptors were released from many proteins and exerted various central effects. Peptides showing anxiolytic-like antihypertensive and anti-alopecia effects via different types of receptors such as OT, FPR and AT2 were also obtained. Based on these study, new functions and post-receptor mechanisms of receptor commom to endogenous and exogenous bioactive peptides have been clarified.

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“…Reduction of the salt content of foods is particularly important for people with high blood pressure. Furthermore, Ohinata et al recently reported that L-arginyl-L-phenylalanine (Arg-Phe) decreased blood pressure and food intake in rodents (4). Arg-Phe does not act as an angiotensin I-converting enzymeinhibitory peptide (5), as is frequently reported, and it shows vasorelaxing activity.…”

mentioning

confidence: 94%

l -Amino Acid Ligase from Pseudomonas syringae Producing Tabtoxin Can Be Used for Enzymatic Synthesis of Various Functional Peptides

Arai

Arimura

Ishikura

et al. 2013

Appl Environ Microbiol

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Functional peptides are expected to be beneficial compounds that improve our quality of life. To address the growing need for functional peptides, we have examined peptide synthesis by using microbial enzymes. L-Amino acid ligase (Lal) catalyzes the condensation of unprotected amino acids in an ATP-dependent manner and is applicable to fermentative production. Hence, Lal is a promising enzyme to achieve cost-effective synthesis. To obtain a Lal with novel substrate specificity, we focused on the putative Lal involved in the biosynthesis of the dipeptidic phytotoxin designated tabtoxin. The tabS gene was cloned from Pseudomonas syringae NBRC14081 and overexpressed in Escherichia coli cells. The recombinant TabS protein produced showed the broadest substrate specificity of any known Lal; it detected 136 of 231 combinations of amino acid substrates when dipeptide synthesis was examined. In addition, some new substrate specificities were identified and unusual amino acids, e.g., L-pipecolic acid, hydroxy-L-proline, and ␤-alanine, were found to be acceptable substrates. Furthermore, kinetic analysis and monitoring of the reactions over a short time revealed that TabS showed distinct substrate selectivity at the N and C termini, which made it possible to specifically synthesize a peptide without by-products such as homopeptides and heteropeptides with the reverse sequence. TabS specifically synthesized the following functional peptides, including their precursors: L-arginyl-L-phenylalanine (antihypertensive effect; yield, 62%), L-leucyl-L-isoleucine (antidepressive effect; yield, 77%), L-glutaminyl-L-tryptophan (precursor of L-glutamyl-L-tryptophan, which has antiangiogenic activity; yield, 54%), L-leucyl-L-serine (enhances saltiness; yield, 83%), and L-glutaminyl-L-threonine (precursor of L-glutamyl-L-threonine, which enhances saltiness; yield, 96%). Furthermore, our results also provide new insights into tabtoxin biosynthesis.

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Novel CCK‐dependent vasorelaxing dipeptide, Arg‐Phe, decreases blood pressure and food intake in rodents

Abstract: A novel CCK-dependent vasorelaxing RF decreases both blood pressure and food intake.

Cited by 43 publications

References 42 publications

Egg White Hydrolysate Can Be a Low-Allergenic Food Material to Suppress Ectopic Fat Accumulation in Rats Fed an Equicaloric Diet

Egg White Hydrolysate Can Be a Low-Allergenic Food Material to Suppress Ectopic Fat Accumulation in Rats Fed an Equicaloric Diet

Bioactive peptides derived from natural proteins with respect to diversity of their receptors and physiological effects

l -Amino Acid Ligase from Pseudomonas syringae Producing Tabtoxin Can Be Used for Enzymatic Synthesis of Various Functional Peptides

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