Novel sequence variants of viral hexon and fibre genes in two dogs with canine adenovirus type 1-associated disease

Balboni, Andrea; Dondi, Francesco; Agnoli, Chiara; Verin, Ranieri; Gruarin, Marta; Morini, Maria; Battilani, Mara

doi:10.1016/j.tvjl.2017.05.011

Cited by 14 publications

(16 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to confirm interspecies transmission or to determine if the same virus could be involved further studies such as sequencing of the virus in the affected species should be carried out. Recent studies have found specific areas of the CAdV‐1 genome which could be studied to find sequence variation of the virus (Balboni et al., ). That would help to understand the epidemiology of the disease.…”

Section: Discussionmentioning

confidence: 99%

Canine adenovirus type 1 (CAdV-1) in free-ranging European brown bear (Ursus arctos arctos ): A threat for Cantabrian population?

Marı́n

Royo

Oleaga

et al. 2018

Transbound Emerg Dis

View full text Add to dashboard Cite

Canine adenovirus type 1 (CAdV-1) is responsible for infectious canine hepatitis. The disease has been described in captive American black bear (Ursus americanus) and European brown bear (Ursus arctos arctos), with just one recently reported case in a cub of a free-ranging brown bear (Ursus arctos horribilis) from Alaska. The aim of this work is to summarize findings related to presence and associated mortality of CAdV-1 in 21 free-ranging Cantabrian brown bears (Ursus arctos arctos) submitted to necropsy in Asturias and Castilla y León (northwestern Spain) from 1998 to 2018. On the basis of the anatomopathological findings and laboratory results three free-ranging brown bears died due to infectious canine hepatitis, which is to our knowledge the first description of death due to this disease in free-ranging bears in Europe. Gross lesions consisted of petechial haemorrhages and congestion in different internal organs, haemorrhagic fluid in internal cavities, friable and yellowish liver and thickening of gall bladder. Microscopic lesions were observed mainly in liver, kidney and brain and consisted of multifocal necrosis of cells with presence of basophilic intranuclear inclusions. Immunohistochemical (IHC) and real-time polymerase chain reaction (qPCR) techniques were used to assess the presence of CAdV-1 in paraffin-embedded liver samples. Viral antigens were detected by IHC labelling within hepatocytes and Küppfer cells in the three animals. The presence of viral DNA was confirmed by qPCR in one of them. In order to evaluate the circulation of CAdV-1 in brown bears, a retrospective study was performed using both IHC and qPCR techniques in 11 and 12 additional brown bears, respectively. An extra brown bear was found positive by IHC. This study shows that CAdV-1 surveillance of brown bears and sympatric carnivores should be considered as major concern for the monitoring the population evolution throughout time in this endangered species.

show abstract

Section: Discussionmentioning

confidence: 99%

Canine adenovirus type 1 (CAdV-1) in free-ranging European brown bear (Ursus arctos arctos ): A threat for Cantabrian population?

Marı́n

Royo

Oleaga

et al. 2018

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…There are two sub-types: CAdV type 1 (CAdV-1) and CAdV type 2 (CAdV-2); CAdV-1 is the etiological agent of infectious canine hepatitis, whereas CAdV-2 causes infectious tracheobronchitis and canine enteritis in domestic dogs (Macartney et al, 1988;Almes et al, 2010). Clinical case reports of CAdV-1 (Headley et al, 2013;Balboni et al, 2017) and CAdV-2 (Hamelin et al, 1986;Spibey and Cavanagh, 1989;Buonavoglia and Martella, 2007) support the hypothesis that CAdV continues to circulate in domestic dogs.…”

Section: Introductionmentioning

confidence: 99%

Development of an Immunochromatographic Strip for Rapid Detection of Canine Adenovirus

Wang

Wen

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

Although canine adenovirus (CAdV) is highly prevalent in dogs, there is currently a lack of a quick diagnostic method. In this study, we developed a rapid immunochromatographic strip (ICS) assay using colloidal gold coupled to CAdV-2-specific monoclonal antibodies (mAbs). BALB/c mice were immunized with a purified CAdV-2 suspension, and four mAbs (belonging to two different epitopes) were generated and designated as 2C1, 7D7, 10D1, and 4G1. Western blot and protein spectral analysis indicated that the hexon protein of CAdV-2 recognized all four mAbs. The colloidal gold-coupled 7D7 and 2C1 mAbs were chosen for inclusion in the rapid ICS assay. The optimal concentrations of the coating antibody (2C1), the capture antibody (7D7), and the goat anti-mouse antibody were 1.0 mg/ml, 10 µg/ml, and 2.0 mg/ml, respectively. The limit of detection was approximately 2.0 × 10 2 tissue culture infective dose (TCID 50 )/ml. Other common canine viruses were tested to evaluate the specificity of the ICS, and positive results were observed for only CAdV-1 and CAdV-2. The ICS test was conducted on 360 samples to detect CAdV, and the results were compared with those of polymerase chain reaction (PCR) tests. The ICS test was found to be a sufficiently sensitive and specific detection method for the convenient and rapid detection of CAdV.

show abstract

“…Both wolf viruses showed a deletion of 41 bps compared to CAdV-1 vaccine strain CLL, whose genome was 3-bps larger than that of CAdV-1 strain R1261 (Sira et al, 1987). The hexon gene revealed the same pattern of amino acid (aa) substitutions observed in previously characterised CAdV strains isolated in Italy from two dogs (strains 574-2013-RS and 417-2013-L) and a red fox (strain 113-5 L) (Balboni et al, 2017). Specifically, the aa mutation from asparagine to serine at position 388 was shared by those Italian isolates, the wolf isolate from this study (Wolf/835/2015/FRA) and the recent Italian wolf isolate (Pizzurro et al, 2017), defining a clear distinctive pattern of substitution.…”

Section: Ngs Analysis and Genome Structurementioning

confidence: 68%

“…Nonetheless, re-emergence of adenovirus infections in dogs has been documented worldwide (Decaro et al, 2004;Benetka et al, 2006;Müller et al, 2010;Balboni et al, 2014;Pintore et al, 2016). Recent evidences suggest the role of foxes as reservoir of CAdV-1 (Balboni et al, 2017;Walker et al, 2016a). Red foxes are the most significant free-ranging wild species in Europe and represent a sympatric species with the domestic dogs, thus playing an important role in disease ecology, due to their high population density and intrusive behaviour (Bateman and Fleming, 2012;Gehrt et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Sequential circulation of canine adenoviruses 1 and 2 in captive wild carnivores, France

Dowgier

Lahoreau

Lanave

et al. 2018

Veterinary Microbiology

View full text Add to dashboard Cite

Scarce data are currently available about the ecology of canine adenoviruses (CAdVs) in wild carnivores. In this paper, the consecutive circulation of CAdV-1 and CAdV-2 in wild carnivores maintained in a French zoological park is reported. A fatal CAdV-1 infection was observed in a Eurasian wolf (Canis lupus lupus), which displayed gross lesions, histopathological changes and immunohistochemical findings suggestive of CAdV-1 infection. The virus was isolated on cell cultures and its genome was determined through next-generation sequencing, resulting genetically related to a recent Italian CAdV-1 strain detected in an Italian wolf. Subsequently, subclinical circulation of CAdV-2 was demonstrated by molecular methods in wild carnivores maintained in the same zoological park, some of which had been previously vaccinated with a CAdV-2 vaccine. Virus detection at a long distance from vaccination and by unvaccinated animals was suggestive of infection by a CAdV-2 field strain, although no data are available about the extent and duration of shedding of CAdV-2 modified-live virus in wild or domestic carnivores. The present paper provides new insights into the CAdV ecology in wildlife, although future studies are needed to fully understand the pathogenic potential of both CAdVs especially in endangered carnivore species.

show abstract

Novel sequence variants of viral hexon and fibre genes in two dogs with canine adenovirus type 1-associated disease

Cited by 14 publications

References 6 publications

Canine adenovirus type 1 (CAdV-1) in free-ranging European brown bear (Ursus arctos arctos ): A threat for Cantabrian population?

Canine adenovirus type 1 (CAdV-1) in free-ranging European brown bear (Ursus arctos arctos ): A threat for Cantabrian population?

Development of an Immunochromatographic Strip for Rapid Detection of Canine Adenovirus

Sequential circulation of canine adenoviruses 1 and 2 in captive wild carnivores, France

Contact Info

Product

Resources

About