Novel SNPs in IL-17F and IL-17A genes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle

Usman, Τahir; Wang, Yachun; Liu, Chao; He, Yanghua; Wang, Xiao; Dong, Yichun; Wu, Hongjun; Liu, Airong; Yu, Ying

doi:10.1186/s40104-016-0137-1

Cited by 13 publications

(13 citation statements)

References 22 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL-17 plays a key role in the host defenses against bacterial and fungal infections [ 12 – 15 ]. Our recent work found that the mutations in IL-17 might serve as biomarkers for resistance against mastitis in Holstein cattle as well as Sanhe cattle, a dual purpose breed of China [ 16 ]. Moreover, IFN-γ is important for immune regulation as well as for the promotion of T cell priming and antibodies production [ 17 – 19 ].…”

Section: Discussionmentioning

confidence: 99%

Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

Khan

Zhang

Liu

et al. 2020

Asian-Australas J Anim Sci

Self Cite

View full text Add to dashboard Cite

Objective: The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period.Methods: The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis.Results: The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and antiinflammation in the periparturient cows in response to coated folic acid treatment.Conclusion: Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows.

show abstract

Section: Discussionmentioning

confidence: 99%

Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

Khan

Zhang

Liu

et al. 2020

Asian-Australas J Anim Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…Serum was isolated from blood samples by incubating the samples at room temperature for 30 min followed by centrifugation at 3000× g for 10 min. The serum samples were then sent to Beijing Huaying Biological Technology Research Institute to evaluate serum biochemical assays of IL-4, IL-6, IL-10, IL-17, TNF-α, and IFN-γ using radioimmunoassay a technique, as described by Usman et al (2017) [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Association of Aberrant DNA Methylation Level in the CD4 and JAK-STAT-Pathway-Related Genes with Mastitis Indicator Traits in Chinese Holstein Dairy Cattle

Usman

Ali

Wang

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

The present study was designed to evaluate the gene expression and DNA methylation level in the promoter region of the CD4 and the JAK-STAT-pathway-related genes. A total of 24 samples were deployed in the gene expression and 118 samples were used in the DNA methylation study. Student’s t-tests were used to analyze the gene expression and DNA methylation. The evaluation of DNA methylation in promoter regions of JAK2 and STAT5A revealed hypo-methylation levels of CpG sites and higher gene expression in cows diagnosed with mastitis as compared to the healthy control, and vice versa in those with CD4. DNA methylation was negatively correlated with gene expression in JAK2, STAT5A, and CD4 genes. Six, two, and four active transcription factors were identified on the CpG sites in the promoter regions of JAK2, STAT5A, and CD4 genes, respectively. Regarding correlation analysis, the DNA methylation levels of CD4 showed significantly higher positive correlations with somatic cell counts (p < 0.05). Findings of the current study inferred that aberrant DNA methylation in the CpG sites at the 1 kb promoter region in JAK2, STAT5A, and CD4 genes due to mastitis in cows can be used as potential epigenetic markers to estimate bovine mastitis susceptibility in dairy cattle.

show abstract

“…The analysis of DEGs associated with inflammation and immunity indicated that the analyzed cows have a localized inflammatory reaction, which was consistent with the determination of SCC. In addition, Tahir Usman et al [40] found that interleukin 17F (IL-17F) and interleukin 17A (IL-17A) could be powerful candidate genes of mastitis resistance and the significant single nucleotide polymorphisms (SNPs) might be useful genetic markers against mastitis in both dairy and dual-purpose cattle. In this study, the expression levels of IL-17F and IL-17A did not change significantly.…”

Section: Discussionmentioning

confidence: 99%

Mammary Transcriptome Profile during Peak and Late Lactation Reveals Differentially Expression Genes Related to Inflammation and Immunity in Chinese Holstein

Han

Fan

Yang

et al. 2020

Animals

View full text Add to dashboard Cite

Simple Summary: Milk somatic cell count, referring to the total number of somatic cells per milliliter of bovine milk, changes regularly during the lactation cycle. The somatic cell count of healthy cows is usually higher in late lactation than in peak lactation. When the inflammatory response in dairy cow mammary gland becomes more intense, the milk somatic cell count increases together with the reduction of milk quality and yield. Autoimmunity was thought to play an important role in the prevention of mastitis in late lactation of dairy cattle. However, the underlying mechanisms related to the gene expression levels during the process remain unknown. In this study, transcriptome sequencing was performed to screen the differentially expressed genes related to the inflammation and immunity in healthy Chinese Holstein mammary glands. Our findings are helpful to understand the physiological functions of mammary inflammation of Chinese Holstein during late lactation.Abstract: Somatic cell count (SCC) in milk is widely used in the dairy industry, as an indicator of the health of mammary gland. While the SCC of dairy cattle was higher in late lactation than in peak lactation, its association with gene expressions of mammary gland were largely unknown. In this study, a transcriptomic sequencing approach and bioinformatics analysis were used to investigate the differential expressed genes (DEGs) associated with inflammation and immunity between peak and late periods of lactation in Chinese Holstein. A total of 446 DEGs (padj < 0.05 and fold change >2) were identified, 50 of which belonged to seven pathways and five terms related to inflammation and immunity. Our data suggested that the activation of nuclear transcription factor-κB (NF-κB) pathway and Toll-like receptor signaling pathway caused inflammatory response, and the activation of chemokine signaling pathway and cytokine-cytokine receptor interaction signaling pathway caused a protective immune response to ensure dairy cows health during late lactation. Our findings deepen the understanding of the molecular mechanism and physiological functions of mammary inflammation in Chinese Holstein during late lactation.

show abstract

Novel SNPs in IL-17F and IL-17A genes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle

Cited by 13 publications

References 22 publications

Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

Association of Aberrant DNA Methylation Level in the CD4 and JAK-STAT-Pathway-Related Genes with Mastitis Indicator Traits in Chinese Holstein Dairy Cattle

Mammary Transcriptome Profile during Peak and Late Lactation Reveals Differentially Expression Genes Related to Inflammation and Immunity in Chinese Holstein

Contact Info

Product

Resources

About