Novel spectrophotometric method for determination of some macrolide antibiotics in pharmaceutical formulations using 1,2-naphthoquinone-4-sulphonate

Ashour, Safwan; Bayram, Roula

doi:10.1016/j.saa.2012.08.024

Cited by 19 publications

(14 citation statements)

References 31 publications

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“…A comparison of these methods with reported methods have been presented (Table 6). It has been observed that, the BCG methods have wider linear ranges as compared to most of the reported methods [11][12][13][14] . These proposed methods are more sensitive compared to some of the established methods as shown by the molar absorptivity 11,14,17 .…”

Section: Drugmentioning

confidence: 99%

“…In ion-pair complex formation, ions of opposite electric charge are produced in solution due to electron transfer. Reported spectrophotometric methods of these three antibiotics includes complex formation with quinalizarin 11 , eosin Y 12 , 2-napthoquinone-4-sulphonate 13 , rose bengal 14 , cresol red 15 , supracene violet 3B, tropaeolin 000 16 , chloranilic acid and 7,7,8,8-tetracyanoquinodimethane 17 .…”

Section: Introductionmentioning

confidence: 99%

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Spectrophotometric Determination of Macrolides Using Bromocresol Green in Pharmaceutical Formulations and Urine Samples

Keskar

Jugade

2015

Analytical Chemistry Letters

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Simple and rapid methods have been developed for the determination of azithromycin, roxithromycin and erythromycin in bulk and pharmaceutical formulations. The methods were based on the formation of bluish-green ion pair complexes of these antibiotics with bromocresol green (BCG) in acetonitrileethanol medium. 2:1 complexes were formed between the drug and reagent almost instantaneously with absorption maxima at 630 nm, 620 nm and 625 nm for the three drugs respectively. Under optimum conditions, calibration curves were found to be linear over the range of 4-46 μg mL -1 , 3-53 μg mL -1 and 7-73 μg mL -1 respectively for the three drugs. The detection limits were found to be 0.19, 0.56 and 0.30 μg mL -1 . The methods were subjected to analytical quality control. Recovery studies and interference studies were carried out. The proposed methods were successfully applied to the determination of these drugs in their pharmaceutical formulations and spiked urine samples.

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Section: Drugmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Macrolides Using Bromocresol Green in Pharmaceutical Formulations and Urine Samples

Keskar

Jugade

2015

Analytical Chemistry Letters

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“…ERY exhibits significant activity against Gram‐positive and Gram‐negative bacteria and is widely used in veterinary medicine to treat respiratory diseases and enteric infections in food‐producing animals . Due to its extensive use, many analytical techniques for the determination of erythromycin in a variety of matrices (biological ; environmental , or pharmaceutical samples) have been published, such as various chromatographic , spectrophotometric , and electrochemical methods. High‐performance liquid chromatography with different modes of detection such as ultraviolet detection and mass spectrometry is one of the analytical methods most frequently used for the detection and quantification of erythromycin, especially in water samples .…”

Section: Introductionmentioning

confidence: 99%

Voltammetric determination of erythromycin in water samples using a boron-doped diamond electrode

Radičová

Behúl

Vojs

et al. 2015

Phys. Status Solidi B

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A new, simple, sensitive, and low‐cost voltammetric method using an as‐grown boron‐doped diamond electrode was developed for the determination of erythromycin in environmental (water) samples. Erythromycin provided an irreversible oxidation peak at a potential of + 0.87 V versus Ag/AgCl/KCl (3 mol/L) electrode in an ammonium acetate buffer (pH 5). Under optimal experimental conditions, square‐wave voltammetry was used for the determination of erythromycin. The calibration curves showed good linearity in a concentration range from 6.8 to 68.1 μmol/L with the coefficient of determination R2 = 0.9993. The limit of detection of the proposed method was 1.1 μmol/L. Very good repeatability (n = 7) at three different concentration levels (6.8, 13.6 and 20.4 μmol/L) was obtained (RSD of 2.4, 1.8, 1.6, respectively). The developed square‐wave method was successfully applied to the determination of erythromycin in several different water samples with good recovery (from 89 to 107%).

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“…Numerous methods have been reported for analysis of AZI and SPI in in raw material, dosage forms and biological fluids, using voltammetry [6] NIR spectroscopy [7], spectrophotometry [8] and spectrofluorimetry [9]. Unfortunately, these methods were associated with some major disadvantages, such as lack of selectivity, time-consuming, tedious, and dedicated to sophisticate and expensive analytical instruments.…”

Section: Introductionmentioning

confidence: 99%

LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples

Mahmoudi

2015

ILNS

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ABSTRACT. Development and validation of rapid HPLC method for quantifying macrolide antibiotics azithromycin (AZI) and spiramycin (SPI) in bulk and tablet samples is described. Determination was performed on a reversed phase C 18 ODB column (250×4.6 nm I.D) at ambient temperature, and employing a UV-detection set at 210 nm. The mobile phase consists of acetonitrile -2-methyl-2-propanol-hydrogenphosphate buffer, pH 6.2, with 1.8% triethylamine (32:8: up to 100, v/v/v), delivered at a flow-rate of 1.1 mL min -1 . The assay is linear in concentration ranges of: 0.004-4.8 and 0.0003-1.2 mg mL −1 for azithromycin and spiramycin, respectively, with detection limit of 0.02% for SPI and 0.03% for AZI. Recovery experiments revealed recovery of 98.51-100.82%. The applicability of this method in stability assessment studies is evaluated.

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Novel spectrophotometric method for determination of some macrolide antibiotics in pharmaceutical formulations using 1,2-naphthoquinone-4-sulphonate

Cited by 19 publications

References 31 publications

Spectrophotometric Determination of Macrolides Using Bromocresol Green in Pharmaceutical Formulations and Urine Samples

Spectrophotometric Determination of Macrolides Using Bromocresol Green in Pharmaceutical Formulations and Urine Samples

Voltammetric determination of erythromycin in water samples using a boron-doped diamond electrode

LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples

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