Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Klak, Marta; Bryniarski, Tomasz; Kowalska, Patrycja; Gomółka, Magdalena; Tymicki, Grzegorz; Kosowska, Katarzyna; Cywoniuk, Piotr; Dobrzański, Tomasz; Turowski, Pawel; Wszoła, Michał

doi:10.3390/mi11070646

Cited by 26 publications

(21 citation statements)

References 147 publications

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“…Three-dimensional cell culture systems provide a consistent research tool and open new avenues for experimental tissue engineering and regenerative medicine in diverse areas of biological and biomedical sciences, including studies of tissue morphogenesis, the efficacy and toxicity of therapeutic compounds in vitro, and drug responses in personalized medicine, with prospects of replacing at least some experimental animal models [59,61,66,80,81,[96][97][98][99]. These 3D cell culture systems also enable the development of new therapeutic approaches to confronting frequent infections in patients with ''The Big Five'' CRD, including COVID-19 (Fig.…”

Section: Lung 3d Cell Cultures and Covid-19mentioning

confidence: 99%

Three-Dimensional Cell Cultures as a Research Platform in Lung Diseases and COVID-19

Costa

Soares

Malagutti-Ferreira

et al. 2021

Tissue Eng Regen Med

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Background: Chronic respiratory diseases (CRD) are a major public health problem worldwide. In the current epidemiological context, CRD have received much interest when considering their correlation with greater susceptibility to SARS-Cov-2 and severe disease (COVID-19). Increasingly more studies have investigated pathophysiological interactions between CRD and COVID-19. Area covered: Animal experimentation has decisively contributed to advancing our knowledge of CRD. Considering the increase in ethical restrictions in animal experimentation, researchers must focus on new experimental alternatives. Two-dimensional (2D) cell cultures have complemented animal models and significantly contributed to advancing research in the life sciences. However, 2D cell cultures have several limitations in studies of cellular interactions. Three-dimensional (3D) cell cultures represent a new and robust platform for studying complex biological processes and are a promising alternative in regenerative and translational medicine. Expert opinion: Three-dimensional cell cultures are obtained by combining several types of cells in integrated and self-organized systems in a 3D structure. These 3D cell culture systems represent an efficient methodological approach in studies of pathophysiology and lung therapy. More recently, complex 3D culture systems, such as lung-on-a-chip, seek to mimic the physiology of a lung in vivo through a microsystem that simulates alveolar-capillary interactions and exposure to air. The present review introduces and discusses 3D lung cultures as robust platforms for studies of the pathophysiology of CRD and COVID-19 and the mechanisms that underlie interactions between CRD and COVID-19.

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Section: Lung 3d Cell Cultures and Covid-19mentioning

confidence: 99%

Three-Dimensional Cell Cultures as a Research Platform in Lung Diseases and COVID-19

Costa

Soares

Malagutti-Ferreira

et al. 2021

Tissue Eng Regen Med

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“…Nowadays, four main bioprinting methods are available: micro-extrusion [ 230 ], inkjet [ 231 ], laser [ 232 ], and stereolithography (SLA) [ 233 ]. The most popular in the field of tissue engineering are currently extrusion-based methods and SLA [ 234 ]. The other important aspects which should be thoughtfully evaluated are, the cell’s concentration in the bioink and proper bioprinting conditions, like pressure, printing speed, or crosslinking method [ 234 ].…”

Section: Artificial Islets and 3d Bioprinted Pancreas For T1d Treatmentmentioning

confidence: 99%

Stem Cells as a Source of Pancreatic Cells for Production of 3D Bioprinted Bionic Pancreas in the Treatment of Type 1 Diabetes

Wszoła¹,

Nitarska²,

Cywoniuk³

et al. 2021

Cells

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Type 1 diabetes (T1D) is the third most common autoimmune disease which develops due to genetic and environmental risk factors. Often, intensive insulin therapy is insufficient, and patients require a pancreas or pancreatic islets transplant. However, both solutions are associated with many possible complications, including graft rejection. The best approach seems to be a donor-independent T1D treatment strategy based on human stem cells cultured in vitro and differentiated into insulin and glucagon-producing cells (β and α cells, respectively). Both types of cells can then be incorporated into the bio-ink used for 3D printing of the bionic pancreas, which can be transplanted into T1D patients to restore glucose homeostasis. The aim of this review is to summarize current knowledge about stem cells sources and their transformation into key pancreatic cells. Last, but not least, we comment on possible solutions of post-transplant immune response triggered stem cell-derived pancreatic cells and their potential control mechanisms.

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“…As OoC models are not the aim of this review, we have not discussed this publication in detail. OoCs have been widely discussed in the literature, so the interested reader is referred to some excellent reviews in this field [219][220][221][222][223][224][225][226]. The idea of OoC was born about 10 years ago and is constantly evolving [227].…”

Section: From Microfluidic Bioreactors Of Complexity Level 3 To Organmentioning

confidence: 99%

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part II: Systems and Applications

et al. 2020

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In this second part of our systematic review on the research area of 3D cell culture in micro-bioreactors we give a detailed description of the published work with regard to the existing micro-bioreactor types and their applications, and highlight important results gathered with the respective systems. As an interesting detail, we found that micro-bioreactors have already been used in SARS-CoV research prior to the SARS-CoV2 pandemic. As our literature research revealed a variety of 3D cell culture configurations in the examined bioreactor systems, we defined in review part one “complexity levels” by means of the corresponding 3D cell culture techniques applied in the systems. The definition of the complexity is thereby based on the knowledge that the spatial distribution of cell-extracellular matrix interactions and the spatial distribution of homologous and heterologous cell–cell contacts play an important role in modulating cell functions. Because at least one of these parameters can be assigned to the 3D cell culture techniques discussed in the present review, we structured the studies according to the complexity levels applied in the MBR systems.

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Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Cited by 26 publications

References 147 publications

Three-Dimensional Cell Cultures as a Research Platform in Lung Diseases and COVID-19

Three-Dimensional Cell Cultures as a Research Platform in Lung Diseases and COVID-19

Stem Cells as a Source of Pancreatic Cells for Production of 3D Bioprinted Bionic Pancreas in the Treatment of Type 1 Diabetes

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part II: Systems and Applications

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