Novel Surface-Modified Bilosomes as Functional and Biocompatible Nanocarriers of Hybrid Compounds

Abstract

In the present contribution, we demonstrate a new approach for functionalization of colloidal nanomaterial consisting of phosphatidylcholine/cholesterol-based vesicular systems modified by FDA-approved biocompatible components, i.e., sodium cholate hydrate acting as a biosurfactant and Pluronic P123—a symmetric triblock copolymer comprising poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO) blocks Eight novel bilosome formulations were prepared using the thin-film hydration method followed by sonication and extrusion in combination with homogenization technique. The optimization studies involving the influence of the preparation technique on the nanocarrier size (dynamic light scattering), charge (electrophoretic light scattering), morphology (transmission electron microscopy) and kinetic stability (backscattering profiles) revealed the most promising candidate for the co-loading of model active compounds of various solubility; namely, hydrophilic methylene blue and hydrophobic curcumin. The studies of the hybrid cargo encapsulation efficiency (UV-Vis spectroscopy) exhibited significant potential of the formulated bilosomes in further biomedical and pharmaceutical applications, including drug delivery, anticancer treatment or diagnostics.

Development and optimization of curcumin analog nano-bilosomes using 21.31full factorial design for anti-tumor profiles improvement in human hepatocellular carcinoma:in-vitroevaluation,in-vivosafety assay

“…BLs efficiency for drug delivery has been confirmed in previous literature, they can improve antigen effectiveness, which is poor when injected, hence, they are healthy and efficient relative to conventional vaccines, bile salts present in the lipid bilayers of BL enhance their resistance against gastrointestinal (GI) bile salts and enzymes, therefore, support protection of the entrapped vaccine against the hostile environment of the GI tract, also, are noninvasive and deliver excellent patient support and compliance. Additionally, Bile salts are absorbed through apical sodium-dependent bile acid transporter (ASBT) in the GIT, which can act as an enhancer in oral delivery (Al-Mahallawi et al., 2015 ; Aburahma, 2016 ; Waglewska et al., 2020 ).…”

No Solid Colloidal Carriers: Aspects Thermodynamic the Immobilization Chitinase and Laminarinase in Liposome

“…Static and dynamic light scattering (DLS), microscopy, size exclusion chromatography (SEC), field flow fractionation (FFF), and centrifugal analysis are used to evaluate the size distribution of liposomes. Electron microscopy or spectroscopic technologies can be used to examine the multilayer structure of liposomes (Waglewska et al, 2020).…”

“…The ethosomes are elastomeric nanovesicles made of phospholipids with a high ethanol concentration (20-45%). (Waglewska et al, 2020). Both hydrophilic and hydrophobic components can be encapsulated in these easily produced lipid vesicles.…”

Hyaluronic Acid-Based Nanocapsules as Efficient Delivery Systems of Garlic Oil Active Components with Anticancer Activity

“…Most studies focused on the molecular effects of these compounds report dissolving DADS [13,14] and DATS [15] in dimethyl sulfoxide (DMSO). However, during the last few decades, there have been an increasing number of works considering the delivery of hydrophobic actives using drug-delivery systems (DDSs) based on, e.g., polymers, including e.g., polysaccharides [16][17][18][19][20], liposomes [21], bilosomes [22]. In particular, the nanoscale systems are of high interest for the encapsulation of hydrophobic pharmaceuticals and nutraceuticals [23][24][25].…”