Novel Synthesis of Aza-phthalimidine Hydroxylactams

Fan, Bo-lin; Liu, Zenglu; Tang, Mei; Xu, Yun; Tang, Xiaowei; Mao, Zhuo

doi:10.1080/00397910802110180

Cited by 5 publications

(1 citation statement)

References 9 publications

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“…6‐Benzyl‐5 H ‐pyrrolo[3,4‐ b ]pyridin‐7(6 H )‐one (3o′): 32 Pale yellow solid; yield: 157.8 mg (0.7 mmol, 23%); mp 153.2–155.1 °C; 1 H NMR (400 MHz, CDCl 3 ): δ =8.80–8.79 (m, 1 H), 7.76–7.74 (m, 1 H), 7.43–7.39 (m, 1 H), 7.37–7.30 (m, 5 H), 4.87 (s, 2 H), 4.27 (s, 2 H); 13 C NMR (100 MHz, CDCl 3 ): δ =166.5, 151.0, 150.8, 136.5, 135.2, 131.3, 129.0, 128.5, 128.0, 125.3, 47.3, 47.0.…”

Section: Methodsmentioning

confidence: 99%

Size‐selective DNA separation: Recovery spectra help determine the sodium chloride (NaCl) and polyethylene glycol (PEG) concentrations required

Xiong

et al. 2014

Biotechnology Journal

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In the presence of sodium chloride (NaCl), DNA fragments can be size-selectively separated by varying the final concentration of polyethylene glycol (PEG). This separation strategy in combination with the use of paramagnetic particles provides a valuable platform for achieving the desired DNA size interval, which is important in automated library preparation for high-throughput DNA sequencing. Here, we report the establishment of recovery spectra of DNA fragments that enable the determination of suitable NaCl and PEG concentrations for size-selective separation. Firstly, at a given NaCl concentration, the recovery equation was obtained by fitting the DNA recovery ratios versus the PEG concentrations using the logistic function to determine the required parameters. Secondly, the slope function of the recovery equation was achieved by deducing its first derivative. Therefore, the recovery spectrum can be generated using the slope function based on those parameters. According to the recovery spectra of different length DNA fragments, suitable NaCl and PEG concentrations can be determined, respectively, by calculating their resolution values and recovery ratios. The strategy was effectively applied to the size-selective separation of 532-, 400-, and 307-bp fragments at the selected reagent concentrations with recoveries of 96.9, 64.7, and 85.9%, respectively. Our method enables good predictions of NaCl and PEG concentrations for size-selective DNA separation.

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Section: Methodsmentioning

confidence: 99%

Size‐selective DNA separation: Recovery spectra help determine the sodium chloride (NaCl) and polyethylene glycol (PEG) concentrations required

Xiong

et al. 2014

Biotechnology Journal

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Potassium Hydroxide‐Catalyzed Chemoselective Reduction of Cyclic Imides with Hydrosilanes: Synthesis of ω‐Hydroxylactams and Lactams

et al. 2016

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Potassium hydroxide-catalyzedh ydrosilylation exhibits excellent activity and chemoselectivity for the reductionofc yclic imidesunder mild reaction conditions.T he chemoselectivity of the reduction systemmay be readily tuned by changingthe identity and stoichiometry of the hydrosilanes:apolymethylhydrosiloxane (PMHS)/potassium hydroxide reduction system resulted in the reductiono fv arious cyclici mides to the corresponding w-hydroxylactams in 70-94% yield,w hile the diphenylsilane (Ph 2 SiH 2 )/ potassium hydroxide reduction system selectively afforded the aryl lactams in 33-95% yield. Thesec atalytic protocols tolerate diverse functional groups and are easy to scale up.

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ChemInform Abstract: Novel Synthesis of Aza‐phthalimidine Hydroxylactams.

et al. 2009

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Fused pyridine derivatives R 0450Novel Synthesis of Aza-phthalimidine Hydroxylactams. -A convenient and mild method for the synthesis of the title compounds involves the substitution reaction of substrates such as (III) with NBS via an unstable intermediate bromide which is rapidly hydrolyzed into the hydroxyl product during the work-up process. A different reaction course is observed in the case of substrate (VI). The yields of (IV) can be improved with NCS/Bz-O-O-Bz. -(FAN, B.; LIU, Z.; TANG, M.; XU, Y.; TANG, X.; MAO*

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Novel Synthesis of Aza-phthalimidine Hydroxylactams

Cited by 5 publications

References 9 publications

Size‐selective DNA separation: Recovery spectra help determine the sodium chloride (NaCl) and polyethylene glycol (PEG) concentrations required

Size‐selective DNA separation: Recovery spectra help determine the sodium chloride (NaCl) and polyethylene glycol (PEG) concentrations required

Potassium Hydroxide‐Catalyzed Chemoselective Reduction of Cyclic Imides with Hydrosilanes: Synthesis of ω‐Hydroxylactams and Lactams

ChemInform Abstract: Novel Synthesis of Aza‐phthalimidine Hydroxylactams.

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