Novel Tertiary Amine Oxide Surfaces That Resist Nonspecific Protein Adsorption

Dilly, Suzanne J.; Beecham, Matthew P.; Brown, Steven P.; Griffin, John M.; Clark, Andrew J.; Griffin, Craig D; Marshall, J.; Napier, Richard; Taylor, Paul C.; Marsh, Andrew

doi:10.1021/la060743j

Cited by 31 publications

(31 citation statements)

References 38 publications

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“…poly-ethylene glycol) and manipulations of the flow cell and tubing that will minimize or eliminate nonspecific binding of the protein to bead column and/or the sidewalls of the flow system, are being investigated. (Dilly et al 2006; Herrwerth et al 2003; Kane et al 2003)…”

Section: Resultsmentioning

confidence: 99%

Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of botulinum neurotoxin using high-affinity antibodies

Warner

Grate

Tyler

et al. 2009

Biosensors and Bioelectronics

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A fluorescence sandwich immunoassay using high affinity antibodies and quantum dot (QD) reporters has been developed for detection of botulinum neurotoxin serotype A (BoNT/A) using a nontoxic recombinant fragment of the holotoxin (BoNT/A-H C -fragment) as a structurally valid simulant for the full toxin molecule. The antibodies used, AR4 and RAZ1, bind to nonoverlapping epitopes present on both the full toxin and on the recombinant fragment. In one format, the immunoassay is carried out in a 96-well plate with detection in a standard plate reader using AR4 as the capture antibody and QD-coupled RAZ1 as the reporter. Detection to 31 pM with a total incubation time of 3 hours was demonstrated. In a second format, the AR4 capture antibody was coupled to Sepharose beads, and the reactions were carried out in microcentrifuge tubes with an incubation time of 1 hour. The beads were subsequently captured and concentrated in a rotating rod "renewable surface" flow cell equipped with a fiber optic system for fluorescence measurements. In PBS buffer, the BoNT/A-H C -fragment was detected to concentrations as low as 5 pM using the fluidic measurement approach.

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Section: Resultsmentioning

confidence: 99%

Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of botulinum neurotoxin using high-affinity antibodies

Warner

Grate

Tyler

et al. 2009

Biosensors and Bioelectronics

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“…A mixture of EP-4 (5.0 g), 3-(dimethylamino)propanoic acid oxide (2.4 g), N,N 0 -dimethylaniline (2 drops) and N-methyl-2-pyrrolidinone (NMP, 10 ml) was stirred at 70°C for 5 h. After cooling to room temperature, the clean viscous solution was slowly dispensed into 50 ml of acetone with stirring to afford a yellowish precipitate [22,23].…”

Section: Synthesis Of the Tertiary Amine Oxide Resinmentioning

confidence: 99%

“…Among above-mentioned works, the water-soluble or thermo-sensitive epoxy resins can be easily obtained by incorporating carboxyl, amines, or amide groups into epoxide molecule. Tertiary amine oxides are widely used in daily chemical, printing and dyeing, paper and leather industries [22,23]. These compounds have a polar non-ionic group, which Scheme 1 Imaging process of the negative working technology Polym.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and properties of a water-soluble thermo-sensitive resin with tertiary amine oxide substituents in the side chain

et al. 2015

Polym. Bull.

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This paper was focused on the synthesis and properties of a watersoluble resin having tertiary amine oxide side substituents. First, 3-(dimethylamino)propanoic acid was synthesized through the addition reaction of dimethylamine with methyl acrylate, followed by hydrolysis of the adduct. Then, 3-(dimethylamino)propanoic acid was oxidized with hydrogen peroxide to obtain 3-(dimethylamino)propanoic acid oxide. Finally, epoxy phenolic was reacted with 3-(dimethylamino)propanoic acid oxide to get the end product. Thermal behaviors and thermo-induced solubility change of the prepared resin were investigated. It was found that the tertiary amine oxide resin was still water soluble even after heating at 120°C for 1 h. However, it readily became completely water insoluble after heating at 170°C for a short period of time. Thin films were prepared with the aqueous solution of the tertiary amine oxide resin and an infrared (IR) dye with maximum absorption at 830 nm. The films could absorb 830 nm IR imaging radiation and give negative image after developing with neutral water. The water-soluble thermosensitive resin might be used in chemical-free thermal laser imaging applications.

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“…Many tail groups have been employed to render surfaces inert to protein and non-specific cellular adhesion. While oligo-ethylene glycol[9–11] has been the standard by which inert surfaces are measured, other groups such as mannitol,[12] maltose,[13] taurin,[14] and tertiary amine oxides[15] have rendered gold surfaces inert. This effect may be due to their ability to order surrounding water molecules, excluding them from the surface and rendering the surface inert to protein adsorption.…”

Section: Introductionmentioning

confidence: 99%

Reduction of 3T3 fibroblast adhesion on SS316L by methyl-terminated SAMs

Raman

Gawalt

2010

Materials Science and Engineering: C

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Inhibiting the non-specific adhesion of cells and proteins to biomaterials such as stents, catheters and guide wires is an important interfacial issue that needs to be addressed in order to reduce surface-related implant complications. Medical grade stainless steel 316L was used as a model system to address this issue. To alter the interfacial property of the implant, self assembled monolayers of long chain phosphonic acids with −CH3, −COOH, −OH tail groups were formed on the native oxide surface of medical grade stainless steel 316L. The effect of varying the tail groups on 3T3 fibroblast adhesion was investigated. The methyl terminated phosphonic acid significantly prevented cell adhesion however presentation of hydrophilic tail groups at the interface did not significantly reduce cell adhesion when compared to the control stainless steel 316L.

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Novel Tertiary Amine Oxide Surfaces That Resist Nonspecific Protein Adsorption

Cited by 31 publications

References 38 publications

Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of botulinum neurotoxin using high-affinity antibodies

Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of botulinum neurotoxin using high-affinity antibodies

Synthesis and properties of a water-soluble thermo-sensitive resin with tertiary amine oxide substituents in the side chain

Reduction of 3T3 fibroblast adhesion on SS316L by methyl-terminated SAMs

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