Novel therapeutic core–shell hydrogel scaffolds with sequential delivery of cobalt and bone morphogenetic protein-2 for synergistic bone regeneration

Pérez, Román A.; Kim, Joong-Hyun; Buitrago, Jennifer O.; Wall, Ivan; Kim, Hae‐Won

doi:10.1016/j.actbio.2015.06.002

Cited by 98 publications

(72 citation statements)

References 38 publications

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“…The expression of Alp, Runx2, Opn, and Ocn were analyzed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The RT-qPCR assay was performed to evaluate the expression levels of the genes of BMSCs cultured with the scaffold at 3, 7 and 14 d. 21 The primer sequences used for PCR amplication are listed in Table 1. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as an internal control, and all primers were synthesized by Invitrogen.…”

Section: Expression Of Osteogenic Genesmentioning

confidence: 99%

Icariin controlled release on a silk fibroin/mesoporous bioactive glass nanoparticles scaffold for promoting stem cell osteogenic differentiation

Shen

Chen

et al. 2020

RSC Adv.

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Section: Expression Of Osteogenic Genesmentioning

confidence: 99%

Icariin controlled release on a silk fibroin/mesoporous bioactive glass nanoparticles scaffold for promoting stem cell osteogenic differentiation

Shen

Chen

et al. 2020

RSC Adv.

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“…Many studies on biomaterial‐assisted bone repair have used induction or modulation, but most of these have been confined to the study of the effect of one biofactor. More recently, inspired by the physiology of bone healing, Raiche et al reported that, compared with single delivery of Co ions incorporated into an alginate‐based shell or bone morphogenetic protein‐2 (BMP‐2) loaded in a collagen‐based core from core–shell fibrous hydrogel, sequential release of the two factors significantly enhanced bone formation . Various delivery vehicles have been developed to regulate the release behaviors of biofactors.…”

Section: Introductionmentioning

confidence: 99%

Multilayered pore‐closedPLGAmicrosphere deliveringOGPandBMP‐2 in sequential release patterns for the facilitation ofBMSCs osteogenic differentiation

Zhang

Han

Duan

et al. 2017

J Biomedical Materials Res

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Bone tissue regeneration may be more effectively administrated by controlled release of multiple biofactors, given that bone healing comprises a cascade of biological events controlled by numerous cytokines and growth factors (GFs). Here, we propose a novel microcarrier with the capability to sequentially deliver dual biofactors for better controlling the bone regeneration process. First, osteogenic growth peptide (OGP) was incorporated in porous poly(lactic-co-glycolic) acid (PLGA) microspheres by a simple solution dipping method and subsequent pore-closing treatment. Then, a multilayered polyelectrolyte coating ((HA-CS) 2 -Hep-BMP-2-Hep-(CS-HA) 2 ) was prepared on the surface of such OGP-loaded pore-closed PLGA microspheres by layer-by-layer assembly. Results showed that the OGP release was minimal (<17.1%) in the first 15 days but accelerated remarkably thereafter, while at least 60.3% of the bone morphogenetic protein-2 (BMP-2) load was released in the first 15 days and only very slow release was observed subsequently. Further in vitro cell experiments showed that the dual-biomolecule-loaded microspheres elicited more cells with extremely elongated cellular morphology, much higher alkaline phosphatase level and upregulated expression of osteocalcin. Such a dual loading of OGP and BMP-2 had a more positive impact on bone marrow mesenchymal stem cells proliferation and osteogenic differentiation compared with either OGP or BMP-2 alone, suggesting potential synergistic benefit of the sequential release of multiple peptide-based biofactors in a coordinated manner. Overall, this dual delivery system may provide a therapeutic strategy sequentially targeting multiple events (or mechanisms) during bone healing, which is believed to benefit the regenerative repair of bone defects.

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“…Hydrogels, a network of polymer chains which can swell in aqueous solutions, hold a large amount of water, permeate to oxygen and nutrients, can be used in the fields of implantable artificial organs, drug delivery, cell encapsulation, biosensors, toxic ion removal and water purification (Cushing and Anseth, 2007;Thakur and Thakur, 2014b;Perez et al, 2015;Thakur and Thakur, 2015). However, most hydrogels are soft.…”

Section: Introductionmentioning

confidence: 99%

Cellulose nanofibers reinforced sodium alginate-polyvinyl alcohol hydrogels: Core-shell structure formation and property characterization

Yue

Han

et al. 2016

Carbohydrate Polymers

140

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Core-shell structured hydrogels consisting of a flexible interpenetrating polymer network (IPN) core and a rigid semi-IPN shell were prepared through chemical crosslinking of polyvinyl alcohol (PVA) and sodium alginate (SA) with Ca(2+) and glutaraldehyde. Short cellulose nanofibers (CNFs) extracted from energycane bagasse were incorporated in the hydrogel. The shell was micro-porous and the core was macro-porous. The hydrogels could be used in multiple adsorption-desorption cycles for dyes, and the maximum methyl blue adsorption capacity had a 10% increase after incorporating CNFs. The homogeneous distribution of CNFs in PVA-SA matrix generated additional hydrogen bonds among the polymer molecular chains, resulting in enhanced density, viscoelasticity, and mechanical strength for the hydrogel. Specifically, the compressive strength of the hydrogel reached 79.5kPa, 3.2 times higher than that of the neat hydrogel.

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Novel therapeutic core–shell hydrogel scaffolds with sequential delivery of cobalt and bone morphogenetic protein-2 for synergistic bone regeneration

Cited by 98 publications

References 38 publications

Icariin controlled release on a silk fibroin/mesoporous bioactive glass nanoparticles scaffold for promoting stem cell osteogenic differentiation

Icariin controlled release on a silk fibroin/mesoporous bioactive glass nanoparticles scaffold for promoting stem cell osteogenic differentiation

Multilayered pore‐closedPLGAmicrosphere deliveringOGPandBMP‐2 in sequential release patterns for the facilitation ofBMSCs osteogenic differentiation

Cellulose nanofibers reinforced sodium alginate-polyvinyl alcohol hydrogels: Core-shell structure formation and property characterization

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