Novel tryptamine-related substances, 5-sulphatoxydiacetyltryptamine, 5-hydroxydiacetyltryptamine, and reduced melatonin in human urine and the determination of those compounds, 6-sulphatoxymelatonin, and melatonin with fluorometric HPLC

Minami, Masayasu; Takahashi, Hideyo; Inagaki, Hirofumi; Yamano, Yuko; Onoue, Sakura; Matsumoto, Shun; Sasaki, Tsukasa; Sakai, Kazuhiro

doi:10.1016/j.jchromb.2009.02.003

Cited by 19 publications

(16 citation statements)

References 27 publications

(45 reference statements)

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“…OH-diatry may have a stress-alleviating action during the day. From the present work we report that: (1) the diurnal changes in the tested tryptamine-related compounds in human urine are detectable even in a stressful situation, and (2) some of these compounds are suitable as stress markers after sleep restriction and psychological task-loading.…”

Section: Introductionmentioning

confidence: 68%

“…OH-diatry and s-diatry have diurnal rhythms of excretion in human urine; their concentrations are highest in the daytime and lowest at night. This evidence was obtained in an experiment with young human subjects in a non-stressful situation [1]; the OH-diatry level during the day was 189 ± 320 (lg/mg creatinine [cr]) and that at night was 71.9 ± 64.1 (lg/mg cr). The difference was statistically significant (p \ 0.01).…”

Section: Introductionmentioning

confidence: 95%

“…Seven tryptamine-related compounds were assayed in the sampled urine by a fluorometric HPLC method [1] and the concentrations were normalized to urinary creatinine levels to control for variations in urine flow and urinary concentrations due to the presence of salts [14].…”

Section: Assay Of Tryptamine-related Compoundsmentioning

confidence: 99%

“…Three novel indolamine compounds, hydroxydiacetyltryptamine (OH-diatry), sulphatoxydiacetyltryptamine (s-diatry), and dihydromelatonin (or reduced melatonin; red mel) have been detected in human urine [1]. Red mel was identified in human urine by our group, as well as being detected in the brains of Goto-Kakizaki diabetic rats by Frese et al [2], and it was tested pharmacologically by Gasparova et al [3] and Ondrejickova et al [4].…”

Section: Introductionmentioning

confidence: 96%

“…The difference was statistically significant (p \ 0.01). In that experiment, all of the subjects resided in special lodging rooms at our institute for 2 days [1]. The excretion rhythm of OH-diatry is opposite to the diurnal rhythm of melatonin [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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The effect of sleep restriction and psychological load on the diurnal metabolic changes in tryptamine-related compounds in human urine

Minami

Takahashi

Sasaki

et al. 2011

Environ Health Prev Med

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Objective The effect of a severely stressful situation (sleep restriction and psychological load) on the diurnal changes in novel tryptamine-related compounds (hydroxydiacetyltryptamine, sulphatoxymelatonin, and dihydromelatonin) was evaluated in human subjects for 16 days. Methods The subjects were allowed to sleep for 5 h on days three through 12 and for 8 h on the other days. On days three through 12, the subjects were asked to perform a psychological task. The first two and the last 4 days were viewed as control days. A performance test was administered to evaluate the extent of the subjects' fatigue. Total urine was sampled by collecting it into bottles three times a day [(1) during the sleeping period, (2) in the morning, and (3) in the afternoon]. Seven tryptamine-related compounds in urine were assayed using HPLC-fluorometry. Results The urine melatonin level was high at night and low during the day. In contrast, urinary levels of hydroxydiacetyltryptamine and sulphatoxydiacetyltryptamine were low at night and high during the day. Dihydromelatonin was undetectable in urine during the sleeping period. Sleep restriction and psychological load did not affect diurnal changes in urinary melatonin, hydroxydiacetyltryptamine, sulphatoxydiacetyltryptamine, or N-acetylserotonin levels. The concentrations of hydroxymelatonin and sulphatoxymelatonin in urine did not show diurnal changes and decreased gradually during the experimental days. A principal component analysis confirmed the diurnal changes and suggested two novel metabolic pathways: (1) N-acetylserotonin to sulphtoxydiacetyltryptamine via hydroxydiacetyltryptamine, and (2) melatonin to dihydromelatonin. Conclusion Severely stressful situations did not affect diurnal changes in melatonin, hydroxydiacetyltryptamine, sulphatoxydiacetyltryptamine, or N-acetylserotonin levels in urine.

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Section: Introductionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 95%

Section: Assay Of Tryptamine-related Compoundsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The effect of sleep restriction and psychological load on the diurnal metabolic changes in tryptamine-related compounds in human urine

Minami

Takahashi

Sasaki

et al. 2011

Environ Health Prev Med

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Up‐to‐date studies regarding the determination of melatonin by chromatographic methods

Alessa

Saber

Althakafy

2021

Archiv der Pharmazie

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Melatonin is an indolic compound that has been reported in the literature to exist in human-based samples, vertebrates, vegetables, fruits, and pharmaceutical products.Melatonin is considered a dietary supplement and can regulate circadian rhythms, although it has not been classified as a drug by the US Food and Drug Administration. Several analytical methods have been used for its detection. This study aimed to summarize the recent outcomes of the chromatographic methods such as electrophoretic methods, gas chromatography, and liquid chromatography, which have been used for the determination of melatonin in the last three decades, with the focus on gas chromatography and high-performance liquid chromatography methods.

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Dietary Indoleamines: Bioavailability and Human Health

Ramakrishna

Bhattacharjee

2021

Food Bioactive Ingredients

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Novel tryptamine-related substances, 5-sulphatoxydiacetyltryptamine, 5-hydroxydiacetyltryptamine, and reduced melatonin in human urine and the determination of those compounds, 6-sulphatoxymelatonin, and melatonin with fluorometric HPLC

Cited by 19 publications

References 27 publications

The effect of sleep restriction and psychological load on the diurnal metabolic changes in tryptamine-related compounds in human urine

The effect of sleep restriction and psychological load on the diurnal metabolic changes in tryptamine-related compounds in human urine

Up‐to‐date studies regarding the determination of melatonin by chromatographic methods

Dietary Indoleamines: Bioavailability and Human Health

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