Novel visible‐light‐induced photocurable tissue adhesive composed of multiply styrene‐derivatized gelatin and poly(ethylene glycol) diacrylate

Li, Cailong; Sajiki, Toshinobu; Nakayama, Yasuhide; Fukui, Masashi; Matsuda, Takehisa

doi:10.1002/jbm.b.10025

Cited by 25 publications

(20 citation statements)

References 15 publications

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“…PSDG has been used for a wide range of biomedical tissue engineering applications including drug release [3-6], peripheral nerve regeneration [5, 6], hemostatic glue [49], cartilage regeneration [50], arterial grafts [51] and hydrogel-coated metallic stents [52]. The results using these PSDG tubes in peripheral nerve injury showing robust axonal regeneration and functional improvements [6] led us to test these scaffolds in completely transected adult rat thoracic spinal cord.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of growth factor- and extracellular matrix-loaded, gelatin-based scaffolds and their biocompatibility with Schwann cells and dorsal root ganglia

Sazo

Maenaka

et al. 2012

Biomaterials

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One of the most exciting new avenues of research to repair the injured spinal cord is to combine cells for implantation with scaffolds that protect the cells and release growth factors to improve their survival and promote host axonal regeneration. To realize this goal, we fabricated biodegradable, photocurable gelatin tubes and membranes for exploratory in vitro studies. Detailed methods are described for their fabrication with a high gelatin concentration. Gelatin membranes fabricated in the same way as tubes and photo-co-immobilized with rhBDNF or rhNT-3, with or without Schwann cells (SCs), showed an initial burst of neurotrophin release within 24h, with release diminishing progressively for 21 days thereafter. SCs attained their typical bipolar conformation on membranes without neurotrophins but adhesion, alignment and proliferation were improved with neurotrophins, particularly rhBDNF. When dorsal root ganglion explants were cultured on membranes containing laminin and fibronectin plus both neurotrophins, neurite outgrowth was lengthier compared to combining one neurotrophin with laminin and fibronectin. Thus, these gelatin membranes allow SC survival and effectively release growth factors and harbor extracellular matrix components to improve cell survival and neurite growth. These scaffolds, based on the combination of cross-linked gelatin technology and incorporation of neurotrophins and extracellular matrix components, are promising candidates for spinal cord repair.

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Section: Discussionmentioning

confidence: 99%

Fabrication of growth factor- and extracellular matrix-loaded, gelatin-based scaffolds and their biocompatibility with Schwann cells and dorsal root ganglia

Sazo

Maenaka

et al. 2012

Biomaterials

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“…91,92 This glue was applied in rats over incised abdominal aortas and it was converted to a gel upon 1 min irradiation with visible light (400 < λ < 500 nm). Haemostasis was completed without complications and the gel gradually degraded within 4 weeks after treatment, showing its suitability for in vivo procedures.…”

Section: Photo-activated Gelatin Gluesmentioning

confidence: 99%

Adhesive biomaterials for tissue reconstruction

Lauto

Mawad

Foster

2007

J of Chemical Tech & Biotech

124

102

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Tissue reconstruction and wound closure rely on sutures, staples and clips in current surgical procedures. These traditional devices are nonetheless unable to prevent leakage of fluids from a variety of tissue including blood vessels and dura mater. Furthermore, sutures are usually difficult to apply during minimal invasive surgery and often induce detrimental scarring that may impair healing. To overcome these disadvantages, biocompatible and biodegradable glues based on fibrin, polyethylene glycol (PEG) and cyanoacrylate have recently been used in patients to seal and repair tissue wounds. Cyanoacrylate glues create typically very strong tissue bonds but have mostly been applied externally for skin wound closure because of their residual cytotoxicity. Other adhesive biomaterials are also emerging; these glues and adhesives are usually based on proteins such as albumin and collagen or polysaccharides like chitosan; these are irradiated with coherent or non-coherent light to trigger their adhesion to tissue. These biomaterial based devices offer significant advantages over sutures, such as their sealing or repairing ability, easy application modality and delivery in situ of compounds for accelerating wound healing. This paper reviews different tissue reconstruction strategies employing adhesive biomaterials currently used in surgical and experimental procedures.

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“…Similarly, the Hubbell group's visible light-induced photopolymerizable glue, based on hydrolytically degradable poly(ethylene glycol) diacrylate derivatives and a visible light-induced photoradical generator (eosin Y), is now commercially available [3]. Other types of polymerizable biomacromolecule derivatives have also been reported [4][5][6][7][8][9][10][11][12][13][14][15][16]. However, these systems usually utilize synthetic polymerizable monomers as their major component.…”

Section: Introductionmentioning

confidence: 96%