Now is the time to test early urinary biomarkers in large-scale human samples

Gao, Youhe

doi:10.1007/s11427-019-9562-y

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…We collected large amounts of urine proteome data using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) (Leng et al, 2017;Gao, 2019). ACE2 was detected and quantified in 80.1% of the human urine samples from 1,925 adults that had undergone routine physical examinations (Table S1 in Supporting Information) and 14.1% of the samples among 284 healthy young children (3.1-6.3 years old) (Table S1 in Supporting Information).…”

mentioning

confidence: 99%

Could urinary ACE2 protein level help identify individuals susceptible to SARS-CoV-2 infection and complication?

Sun

Tian

et al. 2020

Sci. China Life Sci.

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mentioning

confidence: 99%

Could urinary ACE2 protein level help identify individuals susceptible to SARS-CoV-2 infection and complication?

Sun

Tian

et al. 2020

Sci. China Life Sci.

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“…About 10-20 ml of midstream specimens of the first-morning urine was collected to establish the standard operation procedure (SOP) and centrifuged at 2,000 r/min for 10 min. Then, the cells and cell debris were removed and the supernatant was retained, separated, and stored at −80 • C. Urinary proteome data were analyzed by using LC-MS/MS (Leng et al, 2017;Gao, 2019).…”

Section: Urinary Sample Collection and Processingmentioning

confidence: 99%

Label-Free Liquid Chromatography–Mass Spectrometry Proteomic Analysis of Urinary Identification in Diabetic Vascular Dementia in a Han Chinese Population

Chen

Xiao

et al. 2021

Front. Aging Neurosci.

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Objective: This study aimed to identify potential diagnostic biomarkers of diabetic vascular dementia (DVD) and unravel the underlying mechanisms using mass spectrometry (MS).Methods: Label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomic analysis was applied to urine samples from four groups, including 14 patients with vascular dementia (VD), 22 patients with type 2 diabetes mellitus (T2DM), 12 patients with DVD, and 21 normal controls (NCs). Searching the MS data by Proteome Discoverer software (ThermoFisher Scientific; Waltham, MA, USA), protein abundances were analyzed qualitatively and quantitatively and compared between these groups. Combining bioinformatics analysis using Gene Ontology (GO), pathway crosstalk analysis using Kyoto Encyclopedia of Genes and Genomes (KEGG), protein–protein interaction (PPI) network analysis using STRING, and literature searching, the differentially expressed proteins (DEPs) of DVD can be comprehensively judged and were further quantified by receiver operating characteristic (ROC) curve methods.Results: The proteomic findings showed quantitative changes in patients with DVD compared to patients with NC, T2DM, and VD groups; among 4,744 identified urine proteins, 1,222, 1,152, and 1,180 proteins displayed quantitative changes unique to DVD vs. NC, T2DM, and VD, respectively, including 481 overlapped common DEPs. Then, nine unique proteins [including HP, SERPIND, ATP5PB, VNN2, ALDH3A1, U2AF2, C6, A0A5C2GRG5 (no name), and A0A5C2FZ29 (no name)] and two composite markers (CM) (A0A5C2GRG5+U2AF2 and U2AF2+C6) were confirmed by a ROC curve method.Conclusion: This study provided an insight into the potential pathogenesis of DVD and elucidated a method for early detection.

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Blood- and Urine-Based Liquid Biopsy for Early-Stage Cancer Investigation: Taken Clear Renal Cell Carcinoma as a Model

Liu

Zhang

Shao

et al. 2023

Molecular & Cellular Proteomics

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Now is the time to test early urinary biomarkers in large-scale human samples

Cited by 5 publications

References 14 publications

Could urinary ACE2 protein level help identify individuals susceptible to SARS-CoV-2 infection and complication?

Could urinary ACE2 protein level help identify individuals susceptible to SARS-CoV-2 infection and complication?

Label-Free Liquid Chromatography–Mass Spectrometry Proteomic Analysis of Urinary Identification in Diabetic Vascular Dementia in a Han Chinese Population

Blood- and Urine-Based Liquid Biopsy for Early-Stage Cancer Investigation: Taken Clear Renal Cell Carcinoma as a Model

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