NRC-interacting factor directs neurite outgrowth in an activity-dependent manner

Zhao, Xiaosu; Fu, Wai Yuen; Hung, Kwok Wang; Chien, Winnie W.Y.; Li, Z.; Fu, Amy Kit Yu; Ip, Nancy Y.

doi:10.1016/j.neuroscience.2014.12.041

Cited by 5 publications

(1 citation statement)

References 47 publications

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“…Conditional knockout of Zfp335 in neural system led to severely reduced cortical size and impaired neurogenesis. Mechanistically, Zfp335 was required for neural progenitor cell self-renewal and proliferation, and neuronal differentiation ( Yang et al, 2012 ) and neuronal morphogenesis ( Zhao et al, 2015 ). Besides, deficiency of naive T cells in mice carrying a hypomorph allele of Zfp335 ( Zfp335 bloto ) uncovered its role in immune system ( Han et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Zinc finger protein Zfp335 controls early T-cell development and survival through β-selection-dependent and -independent mechanisms

Wang

Jiao

Sun

et al. 2022

eLife

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T cell development in the thymus undergoes the process of differentiation, selective proliferation and survival from CD4-CD8- double negative (DN) stage to CD4+CD8+ double positive (DP) stage prior to the formation of CD4+ helper and CD8+ cytolytic T cells ready for circulation. Each developmental stage is tightly regulated by sequentially-operating molecular networks, of which only limited numbers of transcription regulators have been deciphered. Here we identified Zfp335 transcription factor as a new player in the regulatory network controlling thymocyte development in mice. We demonstrate that Zfp335 intrinsically controls DN to DP transition, as T cell-specific deficiency in Zfp335 leads to a substantial accumulation of DN3 along with reduction of DP, CD4+ and CD8+ thymocytes. This developmental blockade at DN stage results from the impaired intracellular TCRβ expression as well as increased susceptibility to apoptosis in thymocytes. Transcriptomic and ChIP-seq analyses revealed a direct regulation of transcription factors Bcl6 and Rorcβ by Zfp335. Importantly, enhanced expression of TCRβ and Bcl6/Rorc restores the developmental defect during DN3 to DN4 transition and improves thymocytes survival, respectively. These findings identify a critical role of Zfp335 in controlling T cell development by maintaining intracellular TCRβ expression-mediated β-selection and independently activating cell survival signaling.

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Section: Discussionmentioning

confidence: 99%