NRT2.1, a major contributor to cadmium uptake controlled by high-affinity nitrate transporters

Guan, Meiyan; Chen, Mingxue; Cao, Zhenzhen

doi:10.1016/j.ecoenv.2021.112269

Cited by 27 publications

(6 citation statements)

References 52 publications

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“…Nitrate transporter are involved in the constitutive high-affinity transport system (cHATS) under low nitrate conditions. The principal role of this cHATS is to enable roots previously deprived of nitrate to absorb this ion and initiate induction of nitrate-inducible genes [ 83 ]. Cd has been reported to inhibit NO 3 − uptake in several plant species, under normal and high nitrate supply [ 84 ].…”

Section: Discussionmentioning

confidence: 99%

Global leaf and root transcriptome in response to cadmium reveals tolerance mechanisms in Arundo donax L

et al. 2022

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The expected increase of sustainable energy demand has shifted the attention towards bioenergy crops. Due to their know tolerance against abiotic stress and relatively low nutritional requirements, they have been proposed as election crops to be cultivated in marginal lands without disturbing the part of lands employed for agricultural purposes. Arundo donax L. is a promising bioenergy crop whose behaviour under water and salt stress has been recently studied at transcriptomic levels. As the anthropogenic activities produced in the last years a worrying increase of cadmium contamination worldwide, the aim of our work was to decipher the global transcriptomic response of A. donax leaf and root in the perspective of its cultivation in contaminated soil. In our study, RNA-seq libraries yielded a total of 416 million clean reads and 10.4 Gb per sample. De novo assembly of clean reads resulted in 378,521 transcripts and 126,668 unigenes with N50 length of 1812 bp and 1555 bp, respectively. Differential gene expression analysis revealed 5,303 deregulated transcripts (3,206 up- and 2,097 down regulated) specifically observed in the Cd-treated roots compared to Cd-treated leaves. Among them, we identified genes related to “Protein biosynthesis”, “Phytohormone action”, “Nutrient uptake”, “Cell wall organisation”, “Polyamine metabolism”, “Reactive oxygen species metabolism” and “Ion membrane transport”. Globally, our results indicate that ethylene biosynthesis and the downstream signal cascade are strongly induced by cadmium stress. In accordance to ethylene role in the interaction with the ROS generation and scavenging machinery, the transcription of several genes (NADPH oxidase 1, superoxide dismutase, ascorbate peroxidase, different glutathione S-transferases and catalase) devoted to cope the oxidative stress is strongly activated. Several small signal peptides belonging to ROTUNDIFOLIA, CLAVATA3, and C-TERMINALLY ENCODED PEPTIDE 1 (CEP) are also among the up-regulated genes in Cd-treated roots functioning as messenger molecules from root to shoot in order to communicate the stressful status to the upper part of the plants. Finally, the main finding of our work is that genes involved in cell wall remodelling and lignification are decisively up-regulated in giant reed roots. This probably represents a mechanism to avoid cadmium uptake which strongly supports the possibility to cultivate giant cane in contaminated soils in the perspective to reserve agricultural soil for food and feed crops.

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Section: Discussionmentioning

confidence: 99%

Global leaf and root transcriptome in response to cadmium reveals tolerance mechanisms in Arundo donax L

et al. 2022

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“…Moreover, nitrate transporters NPF6.3/NRT1.1, NPF7.3/NRT1.5, and NPF7.2/NRT1.8 are responsive to Cd stress conditions in Arabidopsis , regulating the accumulation of Cd under conditions of both high and normal NO 3 - availability ( Li et al., 2010 ; Chen et al., 2012 ; Mao et al., 2014 ; Wang et al., 2018 ). Further studies have indicated that Cd can suppress the expression of key HATS-related genes including NRT2.1 , NRT2.2 , and NRT2.4 , thereby suppressing the uptake and accumulation of nitrate in roots when nitrate levels are low, which results in a corresponding reduction in root Cd uptake ( Guan et al., 2021 ). This suggests that efforts to control nitrate transporter activity may provide a means of abrogating Cd accumulation when growing crops in soil contaminated with this heavy metal.…”

Section: Biological Processes By Atnrt21mentioning

confidence: 99%

Functional analyses of the NRT2 family of nitrate transporters in Arabidopsis

Xu,

Cheng,

Kong

et al. 2024

Front. Plant Sci.

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Nitrogen is an essential macronutrient for plant growth and development. Nitrate is the major form of nitrogen acquired by most crops and also serves as a vital signaling molecule. Nitrate is absorbed from the soil into root cells usually by the low-affinity NRT1 NO3- transporters and high-affinity NRT2 NO3- transporters, with NRT2s serving to absorb NO3- under NO3–limiting conditions. Seven NRT2 members have been identified in Arabidopsis, and they have been shown to be involved in various biological processes. In this review, we summarize the spatiotemporal expression patterns, localization, and biotic and abiotic responses of these transporters with a focus on recent advances in the current understanding of the functions of the seven AtNRT2 genes. This review offers beneficial insight into the mechanisms by which plants adapt to changing environmental conditions and provides a theoretical basis for crop research in the near future.

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“…The modulation of nitrate uptake systems and the proliferation of lateral roots regulate nitrogen acquisition [ 33 , 34 , 35 , 36 ]. It is generally considered that transporters encoded by the NRT1 and NRT2 families have a low and high affinity for nitrate, respectively [ 37 , 38 , 39 , 40 , 41 ]. Numerous signals regulate the expression of NRT genes.…”

Section: Introductionmentioning

confidence: 99%

Insights on Phytohormonal Crosstalk in Plant Response to Nitrogen Stress: A Focus on Plant Root Growth and Development

Ahmad

Jiang

Zhang

et al. 2023

IJMS

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Nitrogen (N) is a vital mineral component that can restrict the growth and development of plants if supplied inappropriately. In order to benefit their growth and development, plants have complex physiological and structural responses to changes in their nitrogen supply. As higher plants have multiple organs with varying functions and nutritional requirements, they coordinate their responses at the whole-plant level based on local and long-distance signaling pathways. It has been suggested that phytohormones are signaling substances in such pathways. The nitrogen signaling pathway is closely associated with phytohormones such as auxin (AUX), abscisic acid (ABA), cytokinins (CKs), ethylene (ETH), brassinosteroid (BR), strigolactones (SLs), jasmonic acid (JA), and salicylic acid (SA). Recent research has shed light on how nitrogen and phytohormones interact to modulate physiology and morphology. This review provides a summary of the research on how phytohormone signaling affects root system architecture (RSA) in response to nitrogen availability. Overall, this review contributes to identifying recent developments in the interaction between phytohormones and N, as well as serving as a foundation for further study.

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NRT2.1, a major contributor to cadmium uptake controlled by high-affinity nitrate transporters

Cited by 27 publications

References 52 publications

Global leaf and root transcriptome in response to cadmium reveals tolerance mechanisms in Arundo donax L

Global leaf and root transcriptome in response to cadmium reveals tolerance mechanisms in Arundo donax L

Functional analyses of the NRT2 family of nitrate transporters in Arabidopsis

Insights on Phytohormonal Crosstalk in Plant Response to Nitrogen Stress: A Focus on Plant Root Growth and Development

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