Nuclear bodies as proteinaceous structures containing ribonucleoproteins

Dupuy-Coin, A.M.; Kalifat, S. R.; Bouteille, M.

doi:10.1016/s0022-5320(72)90091-3

Cited by 75 publications

(12 citation statements)

References 26 publications

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“…These nuclear bodies closely resemble some of the proteinaceous nuclear bodies previously described cytochemically by Bouteille and coworkers (15,16) who showed that the nuclear bodies in their studies consisted of a filamentous protein capsule and a core that probably contained either RNA or DNA. Such bodies have often been identified in neoplastic cells (15,16). It is not known whether the different forms of nuclear bodies shown in Fig.…”

Section: Methodssupporting

confidence: 78%

“…Total endogenous nuclear RNA polymerase activities were measured by the addition of an aliquot of nuclei g of DNA) to a tube that contained 2. (12)(13)(14)(15)(16) Ci/ mmol) in a final volume of 50 ,l. a-Amanitin was used to determine the amount of activity due to RNA polymerase II. RNA polymerase I activity was determined by subtraction of the a-amanitin-inhibited activity from the total (5).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, it was demonstrated that single injections of nafoxidine or Clomid given to neonatal rats result in multiple reproductive tract abnormalities in the adult animal (8). whether there is any relationship to nuclear bodies and reproductive tract abnormalities is unknown, although it is known that many neoplastic cells contain nuclear bodies (15,16,20). Because we have shown that nuclear bodies are induced by compounds that are known to induce reproductive tract abnormalities and neoplasia, the continued study of nuclear bodies as a possible precursor to the neoplastic state should be of interest.…”

Section: Methodsmentioning

confidence: 99%

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Role of estrogen receptor binding and transcriptional activity in the stimulation of hyperestrogenism and nuclear bodies.

Clark¹,

Hardin²,

Padykula³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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The The nonsteroidal estrogen antagonist nafoxidine is an atypical long-acting estrogen in the immature rat. An injection of nafoxidine causes the translocation of the estrogen receptor from the cytoplasm to the nucleus of uterine cells with a subsequent long-term nuclear retention of the receptor, presumably as a receptor-nafoxidine complex. This long-term nuclear retention of the receptor is accompanied by sustained stimulation of uterine size and long-term increase of nuclear RNA polymerase activities (1-5). A single injection of the physiological hormone 17,B-estradiol also results in nuclear retention of the receptor, stimulation of uterine hypertrophy, and increase of nuclear RNA polymerase activities but these effects are of a much shorter duration than those observed after nafoxidine administration (1-7). It has recently been shown that a single injection of nafoxidine or Clomid, a closely related compound, in neonatal rats causes hyperestrogenization of the reproductive tract which results in the appearance of multiple abnormalities and tumors in the adult animal (8).To relate and extend the above biochemical and histopathological observations to morphological changes that occur in the normal immature rat uterus in response to nafoxidine and estradiol treatment, we examined the ultrastructure of uterine luminal epithelial cells as well as changes in nuclear RNA polymerase activities after long-term exposure to these compounds. Estradiol was dissolved in 0.9% NaCl/1% ethanol, and nafoxidine was dissolved 1% ethanol in distilled water. Alternatively, 50-mg paraffin implants were prepared containing 10% (wt/wt) estradiol by liquifying a known quantity of paraffin, mixing the appropriate quantity of hormone with it, and dropping a uniform volume of the liquid mixture onto a precooled glass surface (9). Vehicle (0.25 ml), estradiol (1.0 Ag), or nafoxidine (50,ug) was administered subcutaneously at various times prior to sacrifice. In experiments involving implants, animals were placed under light ether anesthesia, a small incision was made in the nape of the neck, a paraffin implant was placed subcutaneously, and the incision was closed with one or two sutures. MATERIALS AND METHODSAnimals were sacrificed by cervical dislocation. The uteri were rapidly removed, stripped of adhering fat, weighed, and placed in either ice-cold 0.9% NaCl/5 mM ethylene glycolbis(fi-aminoethyl ether)-N,N'-tetraacetic acid for nuclear isolation or in the appropriate fixative for morphological examination (see below).Isolation of Nuclei. Nuclei were isolated by a modification of the hexylene glycol procedure as described (5).Assay of Endogenous RNA Polymerase Activities. Total endogenous nuclear RNA polymerase activities were measured by the addition of an aliquot of nuclei g of DNA) to a tube that contained 2. (12)(13)(14)(15)(16) Ci/ mmol) in a final volume of 50 ,l. a-Amanitin was used to determine the amount of activity due to RNA polymerase II. RNA polymerase I activity was determined by subtraction of the a-amanit...

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Section: Methodssupporting

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Role of estrogen receptor binding and transcriptional activity in the stimulation of hyperestrogenism and nuclear bodies.

Clark¹,

Hardin²,

Padykula³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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“…Their occurrence in every cell nucleus as a constant nuclear organelle is probable but not proven. Several cytochemical and autoradiographic studies have clearly established that simple nuclear bodies consist essentially of protein (Bouteille et al, 1974;Dupuy-Coin et al, 1972). On the basis of RNase and DNase digestions, moreover, Simar (1969) has inferred that they contain both nucleic acids.…”

Section: Figs 4-7mentioning

confidence: 99%

Nucleic acid compartmentalization within the cell nucleus by in situ transferase‐immunogold techniques

Thiry

1995

Microscopy Res & Technique

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In the present review, we report on recent results obtained by in situ transferase-immunogold techniques as to the ultrastructural distribution of DNA and RNA within the cell nucleus. Special emphasis is placed on the various nucleolar components and the various enigmatic structures of the extranucleolar region: interchromatin granules, coiled bodies, and simple nuclear bodies. These data are discussed in the light of our current understanding of the functional organization of the cell nucleus.

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“…in fact, five types of these structures have been reported in the literature, some of which are considered of nucleolar origin. A relationship between NDB and nucleolar fibrillar centers has also been suggested [9,18]. In plant cells two types of NDB have been identified [31]: (a) nucleolus-associated bodies, in proximity or in contact with the nucteolus; (b) NDB free in the nuclear sap and of higher electrondensity than the first type.…”

Section: Discussionmentioning

confidence: 99%

Human cytomegalovirus nuclear and cytoplasmic dense bodies

Severi

Landini

Cenacchi

et al. 1992

Archives of Virology

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One of the characteristic features of cytomegalovirus (CMV) replication is the formation of cytoplasmic dense bodies. Recent findings revealed similar structures also in the nuclei of CMV-infected cells. By transmission electron microscopy, immuno electronmicroscopy, and cytochemistry, we have studied the morphogenetic steps and macromolecular composition of both structures. Our results show that both structures contain DNA, RNA and viral antigenic proteins. Nuclear dense bodies are probably an expression of a stimulated cellular metabolism, while cytoplasmic dense bodies may represent the site where surplus cellular and viral molecules are stored before being eliminated.

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Nuclear bodies as proteinaceous structures containing ribonucleoproteins

Cited by 75 publications

References 26 publications

Role of estrogen receptor binding and transcriptional activity in the stimulation of hyperestrogenism and nuclear bodies.

Role of estrogen receptor binding and transcriptional activity in the stimulation of hyperestrogenism and nuclear bodies.

Nucleic acid compartmentalization within the cell nucleus by in situ transferase‐immunogold techniques

Human cytomegalovirus nuclear and cytoplasmic dense bodies

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