Nuclear competence for maturation and pronuclear formation in mouse oocytes

Bao, Siqin; Obata, Yayoi; Ono, Yukiko; Futatsumata, Nana; Niimura, Shueo; Kono, Toru

doi:10.1093/humrep/17.5.1311

Cited by 16 publications

(14 citation statements)

References 27 publications

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“…Here, we showed that suppression of MSX1 transcript and protein impaired the development of bovine oocytes and embryos through (Kruip et al 1983, Sato et al 1990, Luther et al 2005, Pant et al 2005 and are more fertile (Xu & Greve 1988, Plachot & Mandelbaum 1990, Bao et al 2002. Reduced oocyte maturation in association with polar body extrusion was also reported by Kruip et al (1983), where formation of polar bodies was observed only in oocytes that were microscopically assessed during 19-25 h post LH peak level compared with those assessed earlier.…”

Section: Discussionmentioning

confidence: 91%

Suppression of the transcription factor MSX1 gene delays bovine preimplantation embryo development in vitro

Tesfaye

Regassa²,

Rings³

et al. 2010

Reproduction

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This study was conducted to investigate the effect of suppressing transcription factor gene MSX1 on the development of in vitro produced bovine oocytes and embryos, and identify its potential target genes regulated by this gene. Injection of long double-stranded RNA (LdsRNA) and small interfering RNA (siRNA) at germinal vesicle stage oocyte reduced MSX1 mRNA expression by 73 and 37% respectively at metaphase II stage compared with non-injected controls. Similarly, injection of the same anti-sense oligomers at zygote stage reduced MSX1 mRNA expression by 52 and 33% at 8-cell stage compared with non-injected controls. Protein expression was also reduced in LdsRNA-and siRNA-injected groups compared with non-injected controls at both stages. Blastocysts rates were 33, 28, 20 and 18% in noninjected control, scrambled RNA (scRNA), LdsRNA-and siRNA-injected groups respectively. Cleavage rates were also significantly reduced in Smartpool siRNA (SpsiRNA)-injected group (53.76%) compared with scRNA-injected group (57.76%) and non-injected control group (61%). Large-scale gene expression analysis showed that 135 genes were differentially regulated in SpsiRNA-injected group compared with non-injected controls, of which 54 and 81 were down-and up-regulated respectively due to suppression of MSX1. Additionally, sequence homology mapping and gene enrichment analysis with known human pathway information identified several functional modules that were affected due to suppression of MSX1. In conclusion, suppression of MSX1 affects oocyte maturation, embryo cleavage rate and the expression of several genes, suggesting its potential role in the development of bovine preimplantation embryos.

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Section: Discussionmentioning

confidence: 91%

Suppression of the transcription factor MSX1 gene delays bovine preimplantation embryo development in vitro

Tesfaye

Regassa²,

Rings³

et al. 2010

Reproduction

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“…Many features are essential for formation of a functional oocyte; these include meiotic maturation, cytoplasmic maturation and the establishment of genomic imprinting [21,26,27]. In mice, oocyte-specific imprinting is naturally established during oocyte growth up to 60-65 µm in diameter [25,28].…”

Section: Discussionmentioning

confidence: 99%

Long-Term Effects of In Vitro Growth of Mouse Oocytes on Their Maturation and Development

et al. 2007

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Abstract. Since very few oocytes grow completely in vivo, in vitro growth (IVG) of ovarian oocytes may provide a new source of functional oocytes. The long-term effects of in vitro maturation (IVM) of oocytes and in vitro culture of fertilized eggs have been reported; however, the effects of IVG of oocytes are unknown. Here in, we report the long-term effects of IVG of oocytes. Ovaries from 1-dayold mice containing non-growing oocytes were cultured for 10 days; the isolated follicles were then cultured for 11 days. Secondary follicles from 10-day-old mice were also cultured for 11 days. The nuclei of oocytes collected from the IVG and Graafiais follicles of adult mice were transferred to enucleated oocytes grown in vivo, respectively. Developmental competence was examined following IVM of the reconstituted oocytes. Chronologically, oocytes of 1-day-old, 10-day-old and adult mice were cultured for 22, 12 and 1 day(s). The result showed that the reconstituted eggs developed into pups at high rates after nuclear transfer and in vitro fertilization (IVF) in all the experimental groups (29-45%). However, the pups from reconstituted eggs containing the nuclei of 22-day cultured oocytes were heavier than the control pups (P<0.05). We concluded that long-term culture of oocytes did not affect their nuclear ability to develop to term; however, fetal growth was affected by the culture duration or culture conditions during the initial phase of follicular growth.

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“…Smaller oocytes will not support meiosis [18] and fail to induce the sperm nuclei to form pronuclei [15]. However, when smaller oocytes were fused to mitotically competent oocytes they underwent maturation, which strongly supports the suggestion that reprogramming modifications and the acquisition of epigenetic changes necessary for oocyte meiosis occur at a late in the oocyte growth phase [19].…”

Section: Nuclear Reprogramming Begins From Oogenesismentioning

confidence: 52%

“…This is a time when the development-associated materials accumulate. The nucleus of the oocyte becomes a large storage organelle (germinal vesicle, GV) containing histones, lamins, pore complexes, transcriptional factors and various small ribosomal nuclear proteins [15]. The cytoplasm of the oocyte drastically increases recruiting and accumulating the maternal origin contents such as certain meiosis-associated cell signals, mRNAs, nucleoplasms, proteins and rRNAs.…”

Section: Nuclear Reprogramming Begins From Oogenesismentioning

confidence: 99%

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Yin¹,

Liu²,

Bou³

et al. 2014

Front. Agr. Sci. Eng.

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Oocytes are unique cells with the inherent capability to reprogram nuclei. The reprogramming of the somatic nucleus from its original cellular state to a totipotent state is essential for term development after somatic cell nuclear transfer. The nuclear-associated factors contained within oocytes are critical for normal fertilization by sperm or for somatic cell nuclear reprogramming. The chromatin of somatic nuclei can be reprogrammed by factors in the egg cytoplasm whose natural function is to reprogram sperm chromatin. The oocyte first obtains its reprogramming capability in the early fetal follicle, and then its capacity is enriched in the late growth phase and reaches its highest capability for reprogramming as fully-grown germinal vesicle oocytes. The cytoplasmic milieu most likely contains all of the specific transcription and/or reprogramming factors necessary for cellular reprogramming. Certain transcription factors in the cytoplast may be critical as has been demonstrated for induced pluripotent stem cells. The maternal pronucleus exerts a predominant, transcriptiondependent effect on embryo cytofragmentation, with a lesser effect imposed by the ooplasm and the paternal pronucleus. With deep analysis of transcriptomics in oocytes and early developmental stage embryos more maternal transcription factors inducing cellular reprogramming will be identified.

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Nuclear competence for maturation and pronuclear formation in mouse oocytes

Abstract: The factors required for pronuclear formation are derived from fully grown GV oocytes, and the transformation from decondensed sperm chromatin to a recondensed male pronucleus is governed by GV-derived factors.

Cited by 16 publications

References 27 publications

Suppression of the transcription factor MSX1 gene delays bovine preimplantation embryo development in vitro

Suppression of the transcription factor MSX1 gene delays bovine preimplantation embryo development in vitro

Long-Term Effects of In Vitro Growth of Mouse Oocytes on Their Maturation and Development

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

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