Nuclear DNA amount in pure species and hybrid willows (<i>Salix</i>): a flow cytometric investigation

Thibault, Jérôme

doi:10.1139/b97-153

Cited by 28 publications

(30 citation statements)

References 38 publications

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“…Countless studies have been canied out which reveal the existence of a relationship between the nuclear DNA content of a particular species and its ecology, distribution, life cycle, biomass production, resistance, habit or severa1 other features (Bennett 1998;Bennett and Leitch 2005). Arnongst these investigations, many workers have focused on re-examining this supposed species-specific constancy, some reporting a considerable intraspecific genome size variation (Rayburn et al 1985;Ceccarelli et al 1992;Thibault 1998) and some others supporting intraspecific C-value stability (Bennett et al 2000;Auckland et al 2001). Up to now, the extent of intraspecific genome size variation is hotly debated (Greilhuber 2005) and some authors attribute such variation only to methodological errors or taxa misidentification (Greilhuber 1998;Ohri 1998).…”

Section: Introductionmentioning

confidence: 99%

Genome size variation in the Artemisia arborescens complex (Asteraceae, Anthemideae) and its cultivars

Garcia¹,

Garnatje²,

Twibell³

et al. 2006

Genome

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Different wild Mediterranean populations of Artemisia arborescens from diverse locations, representing its geographical distribution, as well as some of its well-known cultivars and some specimens cultivated as omamentals in gardens, streets, roads and nurseries, were analysed for genome size estimation. Other closely related species endemic to Macaronesia, A. canariensis, A. argentea and A. gorgonum, were also measured, and their nuclear DNA amount has been related to the biogeography of this group of species. Additionally, five populations of the closely related A. absinthium were analysed to establish comparisons. Measurements, acquired by flow cytometry, ranged from 8.29 pg to 11.61 pg for 2C values. Statistically significant differences of nuclear DNA amounts with respect to factors such as insularity or domestication have been detected. However, a quite low intraspecific genome size variation has been detected in these species. Furthermore, the study also addressed the possible hybrid origins, and possible misidentifications of some of the supposed cultivars ofA. arborescens..

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Section: Introductionmentioning

confidence: 99%

Genome size variation in the Artemisia arborescens complex (Asteraceae, Anthemideae) and its cultivars

Garcia¹,

Garnatje²,

Twibell³

et al. 2006

Genome

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“…Flow cytometry has successfully been applied to the study of hybridization in a number of plant taxa including allotetraploid Glycine (Hammatt et al, 1991) and willow (Thibault, 1998), and homoploid hybrids of Limonium (Morgan et al, 1995). In Hieracium, the hybrid origin of two species (H. brachiatum and H. leptophyton) has been inferred from intermediate DNA content measures (Bräutigam and Bräutigam, 1996).…”

Section: Introductionmentioning

confidence: 99%

Interspecific hybridization among Hieracium species in New Zealand: evidence from flow cytometry

et al. 2004

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Hieracium pilosella (Asteraceae) was accidentally introduced to New Zealand about 100 years ago. Since then it has become an aggressive weed, and an unexpected degree of genetic and genome size variation has been detected; features that might result from interspecies hybridization. We investigated the possibility that H. pilosella has hybridized with related taxa. Of the four other subgenus Pilosella species introduced to New Zealand, H. praealtum is the most abundant and, on morphological and distributional evidence, most likely to be the other parent. Flow cytometry was used to estimate relative genome size for 156 Hieracium plants collected from the wild. Plants assigned to either parental or hybrid morphotypes were found to comprise tetraploid and pentaploid individuals using genome size measurements, and this was confirmed with direct mitotic chromosome counts for a subset of plants. The haploid DNA content of H. praealtum was approximately 22% larger than that of H. pilosella.Putative hybrids that were tetraploid had mean genome sizes equivalent to two H. pilosella and two H. praealtum haploid chromosome sets, implying they were hybrids arising from the fertilization of two reduced gametes. Similar results were obtained from tetraploid hybrids produced by controlled pollination. However, the majority of field hybrids were pentaploid with a genome size equivalent to four H. pilosella and one H. praealtum haploid chromosome sets. We infer that these are not first-generation hybrids but represent successful backcrossing with H. pilosella and/or hybrid-hybrid crossing, and that sexual tetraploid hybrids have been the parents. We note that populations putatively of H. pilosella often comprise apomictic pentaploid hybrids. Significantly, our data indicate the emergence of sexual hybrids that provide further opportunity for gene flow among taxa in this complex.

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“…The latter type of markers offers an almost unlimited supply of molecular traits for distinctive fingerprinting of plant materials without prior knowledge of target DNA sequences. The area of willow research showing the greatest development with respect to the use of molecular marker technology is that of population genetics (Beismann et al, 1997;Triest et al, 1999Triest et al, , 2000Triest, 2001), whereas information on basic genomics is scanty (Thibault, 1997;Triest et al, 1997;Alstrom-Rapaport et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Linkage mapping in tetraploid willows: segregation of molecular markers and estimation of linkage phases support an allotetraploid structure for Salix alba × Salix fragilis interspecific hybrids

et al. 2003

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Salix albaÀSalix fragilis complex includes closely related dioecious polyploid species, which are obligate outcrossers. Natural populations of these willows and their hybrids are represented by a mixture of highly heterozygous genotypes sharing a common gene pool. Since nothing is known about their genomic constitution, tetraploidy (2n ¼ 4x ¼ 76) in willow species makes basic and applied genetic studies difficult. We have used a two-way pseudotestcross strategy and singledose markers (SDMs) to construct the first linkage maps for both pistillate and staminate willows. A total of 242 amplified fragment length polymorphisms (AFLPs) and 50 selective amplifications of microsatellite polymorphic loci (SAMPL) markers, which showed 1:1 segregation in the F 1 mapping populations, were used in linkage analysis. In S. alba, 73 maternal and 48 paternal SDMs were mapped to 19 and 16 linkage groups covering 708 and 339 cM, respectively. In S. fragilis, 13 maternal and 33 paternal SDMs were mapped in six and 14 linkage groups covering 98 and 321 cM, respectively. For most cosegregation groups, a comparable number of markers linked in coupling and repulsion was identified. This finding suggests that most of chromosomes pair preferentially as occurs in allotetraploid species exhibiting disomic inheritance. The detection of 10 pairs of marker alleles from single parents showing codominant inheritance strengthens this hypothesis. The fact that, of the 1122 marker loci identified in the two male and female parents, the vast majority (77.5%) were polymorphic and as few as 22.5% were shared between parental species highlight that S. alba and S. fragilis genotypes are differentiated. The highly difference between S. alba-and S. fragilis-specific markers found in both parental combinations (on average, 65.3 vs 34.7%, respectively) supports the (phylogenetic) hypothesis that S. fragilis is derived from S. alba-like progenitors.

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Nuclear DNA amount in pure species and hybrid willows (Salix): a flow cytometric investigation

Cited by 28 publications

References 38 publications

Genome size variation in the Artemisia arborescens complex (Asteraceae, Anthemideae) and its cultivars

Genome size variation in the Artemisia arborescens complex (Asteraceae, Anthemideae) and its cultivars

Interspecific hybridization among Hieracium species in New Zealand: evidence from flow cytometry

Linkage mapping in tetraploid willows: segregation of molecular markers and estimation of linkage phases support an allotetraploid structure for Salix alba × Salix fragilis interspecific hybrids

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