Nuclear functions of endocytic proteins

Pilecka, Iwona; Banach-Orlowska, Magdalena; Miączyńska, Marta

doi:10.1016/j.ejcb.2007.04.004

Cited by 39 publications

(54 citation statements)

References 90 publications

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“…This partial co-distribution may be correlated with the transcriptional control exerted by endogenous VEGF-A on VEGFR-2 expression. To date, very little information is available linking endocytic proteins to the nuclear transcription machinery (55). Other unknown components may also be involved in the same complex in which endogenous VEGF-A, VEGFR-2, and EEA1 are members.…”

Section: Discussionmentioning

confidence: 99%

Endogenous Vascular Endothelial Growth Factor-A (VEGF-A) Maintains Endothelial Cell Homeostasis by Regulating VEGF Receptor-2 Transcription

Guangqi¹,

Cao²,

Bhattacharya

et al. 2012

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

Endogenous Vascular Endothelial Growth Factor-A (VEGF-A) Maintains Endothelial Cell Homeostasis by Regulating VEGF Receptor-2 Transcription

Guangqi¹,

Cao²,

Bhattacharya

et al. 2012

Journal of Biological Chemistry

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“…Many cytoplasmic regulators of endocytic trafficking have distinct nuclear functions. For example, Epsin, which functions in Clathrinmediated endocytosis and autophagy (Csikos et al, 2009), and the ESCRT III protein Snf7, which functions in the cytoplasm to sort proteins into MVBs, also function in the nucleus to regulate gene expression or chromatin remodeling (Borlido et al, 2009;Pilecka et al, 2007). These proteins shuttle between the nucleus and cytoplasm, and many appear to be exclusively cytoplasmic until nuclear export is blocked.…”

Section: Discussionmentioning

confidence: 99%

Drosophila acinusencodes a novel regulator of endocytic and autophagic trafficking

et al. 2010

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SUMMARYEndosomal trafficking affects many cellular pathways from cell signaling to metabolism, but little is known about how these effects are coordinated. In a genetic screen for mutants affecting endosomal trafficking, we identified Drosophila acinus (dacn; hook-like). Its mammalian homolog Acinus has been implicated in RNA processing and chromatin fragmentation during apoptosis. Loss-of-function analysis of dacn revealed two distinct functions. First, dacn is required for stabilization of early endosomes, thus modulating levels of Notch and Egfr signaling. Second, loss of dacn interferes with cellular starvation responses by inhibiting autophagosome maturation. By contrast, overexpression of dacn causes lethality due to enhanced autophagy. We show that this enhanced autophagy is independent of the Tor pathway. Taken together, our data show that dacn encodes a regulator of endosomal and autophagosomal dynamics, modulating developmental signaling and the cellular response to starvation.

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“…1B). An increasing number of factors are reported to execute such dual functions in both the nucleus and at the endosome (Askree et al, 2004;Berndsen et al, 2008;Pilecka et al, 2007;Stauffer et al, 2001;Weiss et al, 2008;Wu et al, 2005;Xu et al, 2009;Yeghiayan et al, 1995), and we propose that this is also true for Wah. So far, our immunohistochemical attempts failed to localise cytoplasmic HA-Wah to the endosomal machinery (top graph in Fig.…”

Section: Discussionmentioning

confidence: 78%

“…Apart from the core machineries of endocytic trafficking, rather little is known about regulatory factors and links to other cellular processes expected to regulate, refine and orchestrate events of endosomal trafficking in space and time. For example, an increasing number of factors have demonstrated functions in both the nucleus and endosome (Askree et al, 2004;Pilecka et al, 2007), but potential systemic links between both compartments are little explored. Here we report the 170-kD predominantly nuclear factor Drosophila Waharan (Wah) as a novel endosomal regulator in larval muscles.…”

Section: Introductionmentioning

confidence: 99%

The nuclear protein Waharan is required for endosomal-lysosomal trafficking inDrosophila

Lone

Kungl

Koper

et al. 2010

Journal of Cell Science

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SummaryHere we report Drosophila Waharan (Wah), a 170-kD predominantly nuclear protein with two potential human homologues, as a newly identified regulator of endosomal trafficking. Wah is required for neuromuscular-junction development and muscle integrity. In muscles, knockdown of Wah caused novel accumulations of tightly packed electron-dense tubules, which we termed 'sausage bodies'. Our data suggest that sausage bodies coincide with sites at which ubiquitylated proteins and a number of endosomal and lysosomal markers co-accumulate. Furthermore, loss of Wah function generated loss of the acidic LysoTracker compartment. Together with data demonstrating that Wah acts earlier in the trafficking pathway than the Escrt-III component Drosophila Shrb (snf7 in Schizosaccharomyces pombe), our results indicate that Wah is essential for endocytic trafficking at the late endosome. Highly unexpected phenotypes result from Wah knockdown, in that the distribution of ubiquitylated cargos and endolysosomal morphologies are affected despite Wah being a predominant nuclear protein. This finding suggests the existence of a relationship between nuclear functions and endolysosomal trafficking. Future studies of Wah function will give us insights into this interesting phenomenon.

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Nuclear functions of endocytic proteins

Cited by 39 publications

References 90 publications

Endogenous Vascular Endothelial Growth Factor-A (VEGF-A) Maintains Endothelial Cell Homeostasis by Regulating VEGF Receptor-2 Transcription

Endogenous Vascular Endothelial Growth Factor-A (VEGF-A) Maintains Endothelial Cell Homeostasis by Regulating VEGF Receptor-2 Transcription

Drosophila acinusencodes a novel regulator of endocytic and autophagic trafficking

The nuclear protein Waharan is required for endosomal-lysosomal trafficking inDrosophila

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