Nuclear magnetic resonance studies on the biochemistry of biopolymers

Rowe, Janet; Hinton, James F.; Rowe, Karen L.

doi:10.1021/cr60263a001

Cited by 63 publications

(12 citation statements)

References 60 publications

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“…In the area above 5 ppm, two signals with about the same intensity were found at 5.30 and 5.06 ppm (see Table 2), indicating the presence of two C-1 protons in a-glycosidic linkages [30]. At 4.60 ppm a multiplet is observable indicating a further C-I proton but in a j-glycosidic linkage.…”

Section: Anomeric Conjigurution In R4 Oligosuccharidementioning

confidence: 84%

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Structural Studies on the Glucose‐Heptose Region of the Proteus mirabilis R Core

Radziejewska‐Lebrecht

Feige

Jensen

et al. 1980

European Journal of Biochemistry

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Methylation analysis of the core oligosaccharide of the Proteus mirahilis mutant R4 (derived from serotype 028 was carried out in order to obtain information on the internal (glucose-heptose) region of the P. mirahilis R core.The isolated core oligosaccharide was composed of glucose, L-glycero-D-manno-heptose, 3-deoxy-D-munno-octulosonic acid (dOclA) and phosphorus in a molar ratio of about 1 : 2 : 1 : 1.4. It was methylated either directly or after dephosphorylation. To localize the position of the phosphate substituents, the permethylated product was dephosphorylated with hydrogen fluoride and the oligosaccharide obtained was remethylated using CZH31. Location of phosphate at C-7 of the terminal heptose was shown by isolation of the sugar phosphate from partial hydrolysates and gasliquid chromatography/mass spectrometry of the permethylated product.Combining the data of the methylation analysis with the data of an NMR study allows one to formulate the structure of the core oligosaccharide as follows :DGlcp(fll+3/4)~cc~Hepp(l + ?)dOclA 0-specific chains of enterobacterial lipopolysaccharides vary highly in composition and structure amongst the various serotypes [l]. In contrast, the R core region of Enterobacteriaceae lipopolysaccharides is of rather limited variability, e.g. all Salmonella serotypes seem to share the same R core and in Eschericliia coli, Skigella and Citrobacter five different R core types have been encountered so far [2 -71, which, however, resemble each other and the Salmonella core in composition and structure. Some of these types are identical for several genera of the Enterobacteriaceae family [8]. The differences refer to the external core regions, while the internal region (heptose/dOclA region) seem to be identical. This can be deduced by the sensitivity of deep R mutants from different genera versus a whole range of R-specific phages like FP1, FP3, C21 and $X 174 [5,9]. All hitherto investigated R mutants of P. mirahilis are resistant to the action of the following (R) phages: Felix 01, 6SR, Br2, BrlO, Ffm, Fpl, Fp3 and P22, indicating that differences exist in the phage-receptor region of P. mirahilis R mutants and those of other enterobacterial genera like Salmonella and Esclzerichiu [lo].From P. miruhilis 028 (strain F87) an R mutant was isolated and designated as R4 mutant [10,13]. Its lipopolysaccharide contains only D-glucose, ~-g l y crro-r>-manno-heptose and dOclA, in addition to lipid A.

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Section: Anomeric Conjigurution In R4 Oligosuccharidementioning

confidence: 84%

“…For the determination of the anomeric linkages in the R4 core oligosaccharide, proton NMR spectroscopy was carried out [30] using a Bruker HFX-90 (90-MHz PMR instrument) operated at 70 "C [31] with sodium 3-trimethylsilyl-(2,2,3,3-2H4)propionate as internal standard.…”

Section: N M R Studiesmentioning

confidence: 99%

Structural Studies on the Glucose‐Heptose Region of the Proteus mirabilis R Core

Radziejewska‐Lebrecht

Feige

Jensen

et al. 1980

European Journal of Biochemistry

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“…The anomeric protons of the carbohydrate resonate in the region 4-5ppm (Rowe et al 1970). The Table 3 Ranges of o-value for chemical shifts of signals from protons in various lignin preparations proton shifts due to the hydroxyl protons can also be observed in this region, overlapping each other and superimposing on the C-H proton peak.…”

Section: Resultsmentioning

confidence: 99%

Studies on a Lignin-Carbohydrate Complex. Pt. II. Characterisation of the Water-Soluble Lignin-Carbohydrate Complex

Merewether¹,

Samsuzzaman²,

Calder³

1972

Holzforschung

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“…1 reveals two triplets due to the a-proton at (5 3.71 ppm and the c-protons at (5 3.01 ppm, and a multiplet due to the overlappings of the six protons of the three methylenes, {J, rand (5. Prior to addition of metal to this solution, the chemi- cal shifts were measured in detail in the pD region from 0 to 14. The pD dependence of the chemical shifts is shown in Fig.…”

Section: Pka Determinationmentioning

confidence: 99%

Interaction of Amino Acids with Transition Metal Ions in Solution (I) Solution Structure ofl-Lysine with Co(II) and Cu(II) Ions as Studied by Nuclear Magnetic Resonance Spectroscopy

Ishida

Ōkubo

Kawai

et al. 1980

Agricultural and Biological Chemistry

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Nuclear magnetic resonance studies on the biochemistry of biopolymers

Cited by 63 publications

References 60 publications

Structural Studies on the Glucose‐Heptose Region of the Proteus mirabilis R Core

Structural Studies on the Glucose‐Heptose Region of the Proteus mirabilis R Core

Studies on a Lignin-Carbohydrate Complex. Pt. II. Characterisation of the Water-Soluble Lignin-Carbohydrate Complex

Interaction of Amino Acids with Transition Metal Ions in Solution (I) Solution Structure ofl-Lysine with Co(II) and Cu(II) Ions as Studied by Nuclear Magnetic Resonance Spectroscopy

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