Nuclear morphometry of neoplastic cells as a method for diagnosis of histiocytoma, mastocytoma and transmissible venereal tumor in dogs

Waldemarin, Katia Cristina de Andrade; Beletti, Marcelo Emı́lio; Costa, Luciano da Fontoura

doi:10.1016/j.rti.2004.05.003

Cited by 11 publications

(11 citation statements)

References 17 publications

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“…The usefulness of computer‐assisted morphometry in veterinary medicine has been recognized in the diagnosis of canine mast cell tumours (Strefezzi et al., 2003; Maiolino et al., 2005), squamous cell carcinomas of skin (Maiolino et al., 2002), canine round cell tumours (Waldemarin et al., 2004), canine and feline melanocytic tumours (Destexhe et al., 1995; Roels et al., 2000), feline mammary carcinomas (De Vico and Maiolino, 1997), canine seminomas (Maiolino et al., 2004) and canine mammary gland tumours (Ciurea et al., 1992; Destexhe et al., 1995; Simeonov and Simeonova, 2006a,b, 2007). Moreover, several studies have outlined nuclear morphometric analysis as a valuable diagnostic predictor in various cancer in small animals (De Vico and Maiolino, 1997; Maiolino et al., 2002, De Vico et al., 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Use of Quantitative Analysis as a Method for Differentiation between Canine Cutaneous Apocrine Adenomas and Apocrine Carcinomas on Cytological Smears

Simeonov

Simeonova

2007

Journal of Veterinary Medicine Series A

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Eight canine cutaneous adenomas and eight canine cutaneous carcinomas were analysed by computer-assisted nuclear morphometry in Hemacolor-stained cytological specimens. In each case, the nuclei of at least 100 neoplastic cells were measured, and the mean nuclear area (MNA), mean nuclear perimeter (MNP), mean nuclear diameter (MND) and nuclear roundness (NR) were calculated. The results indicated an increase in the mean values of investigated parameters from canine cutaneous apocrine adenomas (MNA, 75.65 ± 2.22; MNP, 31.05 ± 0.55; MND, 9.62 ± 0.14; NR, 1.10 ± 0.009) to canine cutaneous apocrine carcinomas (MNA, 88.78 ± 11.29; MNP, 34.38 ± 2.43; MND, 10.43 ± 0.76; NR, 1.21 ± 0.07). The statistical analysis revealed statistically significant differences between benign and malignant neoplastic cells (P < 0.01). The statistical differences between investigated parameters (P < 0.01) were also found between the metastasizing apocrine carcinomas and all other examined carcinomas. The results indicated that the computerized morphometry could be used as an effective auxiliary tool for differential diagnosis between canine cutaneous adenomas and carcinomas on cytological smears. www.blackwell-synergy.com

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Section: Discussionmentioning

confidence: 99%

Use of Quantitative Analysis as a Method for Differentiation between Canine Cutaneous Apocrine Adenomas and Apocrine Carcinomas on Cytological Smears

Simeonov

Simeonova

2007

Journal of Veterinary Medicine Series A

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“…Morphometry is the quantitative description of geometric figures of cellular structures in any dimension. In recent years the development of computerized quantitative image analysis in veterinary oncology proved useful for nuclear morphometric analysis in the diagnosis of canine mast cell tumours (Strefezzi et al, 2003), feline squamous-cell carcinomas , canine round cell tumours (Wandemarin et al, 2004), canine and feline melanocytic tumours (Roels et al, 2000), feline mammary carcinomas (De Vico and Maiolino, 1997) and canine mammary gland tumours (Ciurea et al, 1992;Destexhe et al, 1995). Computerassisted image analysis can also be used for assessement of nuclear organized regions (AgNOR) in various small animal tumours (Harmelin, 1995;Preziosi et al, 1995;Hung et al, 2000;Juntes and Pogacnic, 2000), as well as for quantitative determination of intratumoural vessel density (Griffey et al, 1998;Maiolino et al, 2001;Kent et al, 2002;Preziozi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Computerized Cytomorphometric Analysis of Nuclear Area, Nuclear Perimeter and Mean Nuclear Diameter in Spontaneous Canine Mammary Gland Tumours

Simeonov

Simeonova

2007

Vet Res Commun

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Fifty-two spontaneous canine mammary gland tumours (fibroadenomas (n=8), tubulopapillary carcinomas (n=9), solid carcinomas (n=6), anaplastic carcinomas (n=7), fibrosarcomas (n=9), liposarcomas (n=9) and osteosarcomas (n=4) were analysed by computer-assisted nuclear morphometry in Hemacolor-stained cytological specimens. Computerized cytomorphometry was performed and the nuclear area, nuclear perimeter and mean nuclear diameter of investigated tumours were assessed. A minimum of 100 nuclei per lesion were examined. The statistical analysis revealed statistically significant differences between benign and malignant neoplasms. The results indicated that computer-assisted nuclear morphometry could be used as an additional method for differentiation of benign from malignant canine mammary gland tumours in cytological specimens.

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“…The images were captured and magnified 40X using an Olympus BX 40 optical microscope with an Olympus OLY 200 camera. For threshold segmentation or contact (Waldemarin, Beletti, & Costa, 2004), binary images were formed and the percentage of black color was calculated in delimited areas that show the region of the globet cells by the program of analysis of HL Image 97 images (Western Vision Softwares).…”

Section: Percentage Of Goblet Cells Per Delimited Areamentioning

confidence: 99%

Antimicrobial effect of bothropstoxin-i in broilers

Silveira

Gimenes

Barbaresco³

et al. 2021

Semina: Ciênc. Agrár.

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Bacterial resistance is a sanitary issue explained by indiscriminate use of nonprescription drugs, and antimicrobial use in food production for growth promotion. Bothropstoxin-I (BthTx-I) is a phospholipase A2 (PLA2) from Bothrops jararacussu venom, which has a known antimicrobial effect. The goal of this study was the unprecedented evaluation of in vivo antimicrobial activity of BthTx-I in broilers. Microbiological, biochemical, and histological parameters were determined using 84 21-day old broilers that were kept in cages with four birds each at a density of 625 cm2/broiler. The experiment was randomized by three treatments with seven repetitions of four broilers each that lasted seven days. The treatments were: 1) bacitracin zinc diet; 2) PLA2-BthTx-I; 3) without additives. The data obtained from the studied variables was subjected to analysis of variance and an F-test at the 5% significance level. Averages of each variable in each treatment were compared by Tukey’s test. Broiler bacterial cloacal counts showed that BthTx-I decreased the microbial population without reducing body weight, intestinal morphology, or liver or kidney histopathological damage. The toxin showed in vivo activity, being an alternative for better performance in the production of broiler chickens, because it acted by decreasing the microbial load of potentially pathogenic bacteria in the intestinal microbiota of the birds and did not cause muscle, liver or kidney damage at the assessed dosage.

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Nuclear morphometry of neoplastic cells as a method for diagnosis of histiocytoma, mastocytoma and transmissible venereal tumor in dogs

Cited by 11 publications

References 17 publications

Use of Quantitative Analysis as a Method for Differentiation between Canine Cutaneous Apocrine Adenomas and Apocrine Carcinomas on Cytological Smears

Use of Quantitative Analysis as a Method for Differentiation between Canine Cutaneous Apocrine Adenomas and Apocrine Carcinomas on Cytological Smears

Computerized Cytomorphometric Analysis of Nuclear Area, Nuclear Perimeter and Mean Nuclear Diameter in Spontaneous Canine Mammary Gland Tumours

Antimicrobial effect of bothropstoxin-i in broilers

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