Nuclear translocation of PDCD5 (TFAR19): an early signal for apoptosis?

Chen, Yingyu; Sun, Ruoyan; Han, Wenling; Zhang, Yingmei; Song, Quansheng; Di, Chunhui; Ma, Dalong

doi:10.1016/s0014-5793(01)03062-9

Cited by 99 publications

(98 citation statements)

References 20 publications

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“…Nineteen of these genes are also down-regulated in our INV and MET libraries in a significant way, and they are as follows: PNRC1, CEBPD, TM4SF1, ANXA1, TNFRSF10B, RASD1, CXCL1, IL8, TFF1, SCGB3A1, LIF, LAMC2, CXCL3, CCL20, CXCL6, KRT6B, SOD2, SAT, and STC2; the remaining 13 are also down-regulated in our INV and MET libraries, but are not reported in Tables 1 and 3 Additional genes down-regulated in Table 1 are not associated to breast cancer but are described to be important in the genesis of other tumor types. They include the following: the tumor suppressor genes: MSF, DOC1R (a potential human tumor suppressor gene), SUI1 (a putative translation initiation factor), and ST13 (also called SNC6) (14 -17); genes involved in the apoptotic pathway are as follows: GADD45B and PDCD5 (18,19); genes controlling cell proliferation are: LIF, which regulates the growth of normal human breast epithelial cells, and SEMA3B, a mediator of p53 tumor-suppressor activity (20 -23).…”

Section: Sage Librarymentioning

confidence: 99%

Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis

Zucchi

Mento

Kuznetsov

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto, which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (Ϸ500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer.breast cancer ͉ serial analysis of gene expression ͉ cell microdissection ͉ carcinoma

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Section: Sage Librarymentioning

confidence: 99%

Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis

Zucchi

Mento

Kuznetsov

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…PDCD5 is widely expressed in a variety of tissues with its mRNA expression in fetal tissues being significantly lower than that observed in adult tissues. The expressed PDCD5 protein has been shown to traverse rapidly from the cytoplasm to the nucleus of cells and distribute uniformly in cells that undergo apoptosis [2] . Functional studies show that the overexpression of PDCD5 facilitates programmed cell death triggered by growth factor withdrawal or serum deprivation in various types of tumor cells.…”

Section: Discussionmentioning

confidence: 99%

“…PDCD5 is widely expressed in a variety of tissues with its mRNA expression in fetal tissues being significantly lower than that observed in adult tissues. The expression of PDCD5 protein in cells undergoing 11111111 apoptosis is significantly increased and the appearance of PDCD5 in the nuclei of apoptotic cells1precedes the externalization of phosphatidylserine and fragmentation of chromosomal DNA [2] .…”

Section: Introductionmentioning

confidence: 99%

Determination of PDCD5 in peripheral blood serum of cancer patients

Wang

Zhang

2011

Chin. J. Cancer Res.

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Objective: Programmed cell death 5 (PDCD5) is an apoptosis related gene and plays an important role in the pathogenesis and development of cancer. Whether PDCD5 is present in peripheral blood serum has not been reported. The aim of this study is to determine the contents of PDCD5 protein in peripheral blood serum of cancer patients, as well as normal subjects.Methods: ELISA was used to detect the serum PDCD5 concentrations in 100 normal persons, 83 patients with breast cancer, 74 patients with gastrointestinal tract cancer and 41 patients with lung cancer. The results were statistically analyzed and discussed.Results: PDCD5 could be detected in peripheral blood serum in both normal subjects and cancer patients. The serum PDCD5 contents in normal persons ranged from 3.8 to 6.1 ng/ml with a median of 4.70±0.68 ng/ml. For cancer patients the PDCD5 levels were 4.59±0.90, 4.79±1.14 and 10.43±22.34 ng/ml for breast cancer, gastrointestinal cancer and lung cancer patients respectively. There was no statistically significant difference between the serum PDCD5 concentrations of normal persons and cancer patients.Conclusion: PDCD5 is present in peripheral blood. The PDCD5 levels in cancer patients are not statistically different from that of normal persons, though decreased expression of PDCD5 in malignant tissues has been found.

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“…In several studies, increased PDCD5 expression is accompanied by its translocation from the cytoplasm to the nucleus, a process that occurs prior to cell death. Thus, this nuclear translocation of PDCD5 may be an early apoptotic event playing an important role in regulating this process (16). Using both antisense gene knockdown and protein inhibition, PDCD5 was found to be a positive regulator of the apoptotic protein caspase-3.…”

Section: Introductionmentioning

confidence: 99%

Construction and characterization of a PDCD5 recombinant lentivirus vector and its expression in tumor cells

et al. 2012

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Abstract. The function and mechanism of the programmed cell death 5 (PDCD5) gene is not completely understood, so it is necessary to build a stable and efficient PDCD5 recombinant lentiviral expression vector. The coding region of the PDCD5 gene was PCR amplified from pCMV-SPORT6. Next, the PDCD5 fragment and the pGC-FU vector were digested with the restriction enzyme AgeI and ligated by in-Fusion technology to build the pGC-FU-PDCD5 plasmid. Competent E. coli DH5α cells were transformed and positive clones were identified by PCR. The PCR products were digested and sequenced. After sequencing, positive clones were selected to grow and propagate. The pGC-FU-PDCD5 plasmid DNA was purified and mixed with the pHelper1.0 and pHelper2.0 packaging plasmids. They were co-transfected into 293T cells. The viral titer was measured by real-time PCR. The expression of GFP and PDCD5 was detected by both Western blotting and fluorescence microscopy. Additionally, the A498, ACHN, HCT116 and LoVo tumor cell lines were transfected with the virus supernatant, and PDCD5 expression was detected in these cells. The constructed pGC-FU-PDCD5 plasmid contained the correct PDCD5 gene sequence. Strong green fluorescence in both the cytoplasm and cell membrane was observed following transfection with purified pGC-FU-PDCD5 into 293T cells. The transfection rate was greater than 95%, and the expression of the PDCD5-GFP fusion protein was confirmed by Western blotting. The titer of the recombinant PDCD5 lentiviral condensed supernatant was measured to be 2x10 9 Tu/ml. The transfection efficiencies of A498, ACHN and HCT116 cells were greater than 90%. However, transfection efficiency of LoVo cells was lower but still greater than 70%. The PDCD5-GFP fusion protein was stable in these transfected tumor cells, as detected by Western blotting. In conclusion, a PDCD5 recombinant lentiviral vector was successfully constructed, and high expression of the plasmid was observed in tumor cells.

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Nuclear translocation of PDCD5 (TFAR19): an early signal for apoptosis?

Cited by 99 publications

References 20 publications

Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis

Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis

Determination of PDCD5 in peripheral blood serum of cancer patients

Construction and characterization of a PDCD5 recombinant lentivirus vector and its expression in tumor cells

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