Nuclear transplantation, the conservation of the genome, and prospects for cell replacement

Gurdon, J. B.

doi:10.1111/febs.13988

Cited by 11 publications

(5 citation statements)

References 20 publications

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“…Ever since the cloning of the first mammal "Dolly the sheep" (Gurdon, 2017) there has been a huge interest and development in stem cell research for regenerative medicine. The "stem cells" are self-replicating, undifferentiated master cells that are capable of differentiating and producing daughter cells that make up every different cellular and tissue type in the body (Rognoni and Watt, 2018).…”

Section: Regeneration and Stem Cellsmentioning

confidence: 99%

From the raw bar to the bench: Bivalves as models for human health

Robledo

Yadavalli

Allam

et al. 2019

Developmental & Comparative Immunology

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Bivalves, from raw oysters to steamed clams, are popular choices among seafood lovers and once limited to the coastal areas. The rapid growth of the aquaculture industry and improvement in the preservation and transport of seafood have enabled them to be readily available anywhere in the world. Over the years, oysters, mussels, scallops, and clams have been the focus of research for improving the production, managing resources, and investigating basic biological and ecological questions. During this decade, an impressive amount of information using high-throughput genomic, transcriptomic and proteomic technologies has been produced in various classes of the Mollusca group, and it is anticipated that basic and applied research will significantly benefit from this resource. One aspect that is also taking momentum is the use of bivalves as a model system for human health. In this review, we highlight some of the aspects of the biology of bivalves that have direct implications in human health including the shell formation, stem cells and cell

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Section: Regeneration and Stem Cellsmentioning

confidence: 99%

From the raw bar to the bench: Bivalves as models for human health

Robledo

Yadavalli

Allam

et al. 2019

Developmental & Comparative Immunology

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“…4). The scientific basis for this breakthrough actually dates back to the early 1960s with the landmark discovery of Sir John B. Gurdon that specialization of cells can be reversible [19,20]. This conclusion was based on Gurdon's elegant studies whereby the nucleus of a fertilized egg cell from a frog was removed and replaced with the nucleus of a cell taken from a tadpole's intestine.…”

Section: Induced-pluripotent Stem Cells-potential Applicationsmentioning

confidence: 99%

“…This conclusion was based on Gurdon's elegant studies whereby the nucleus of a fertilized egg cell from a frog was removed and replaced with the nucleus of a cell taken from a tadpole's intestine. This modified egg cell grew into a new frog, proving that the mature cell still contained the genetic information needed to form all types of cells [19,20]. Relying on this information, Yamanaka and colleagues [21] identified conditions that would allow some specialized adult cells to be reprogrammed to assume a stem cell-like state-iPSCs.…”

Section: Induced-pluripotent Stem Cells-potential Applicationsmentioning

confidence: 99%

Stem cells and tooth regeneration: prospects for personalized dentistry

et al. 2019

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Over the last several decades, a wealth of information has become available regarding various sources of stem cells and their potential use for regenerative purposes. Given the intense debate regarding embryonic stem cells, much of the focus has centered around application of adult stem cells for regenerative engineering along with other relevant aspects including use of growth factors and scaffolding materials. The more recent discovery of tooth-derived stem cells has sparked much interest in their application to regenerative dentistry to treat and alleviate the most prevalent oral diseases-i.e., dental caries and periodontal diseases. Also exciting is the advent of induced pluripotent stem cells, which provides the means of using patient-derived somatic cells for their creation, and their eventual application for generation of the dental complex. Thus, evolving developments in the field of regenerative dentistry indicate the prospect of constructing Bcustom-made^tooth and supporting structures thereby fostering the realization of Bpersonalized dentistry.^On the other hand, others have explored the possibility of augmenting endogenous regenerative capacity through utilization of small molecules to regulate molecular signaling mechanisms that mediate regeneration of tooth structure. This review is focused on these aspects of regenerative dentistry in view of their relevance to personalized dentistry.

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“…Epigenetic reprogramming erases the parental epigenomes inherited from the gametes and establishes the chromatin landscape of the progenitor of a new generation. In animals, a key role for reprogramming is the establishment of totipotency of the single-celled zygote via the germ cells to allow it to produce all cell types within an organism (Farhadova et al, 2019;Gurdon, 2017;Hemberger et al, 2009;Irie et al, 2018). Failures in reprogramming result in disease (Tucci et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Epigenetic reprogramming of imprinting at meiosis

Montgomery

Berger

2023

Preprint

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In mammals, genomic imprinting results from different sets of epigenetic marks that distinguish the parental origins of loci in the progeny. Epigenetic reprogramming of genomic imprinting is necessary to establish a totipotent cell state. The consecutive erasure of parental epigenetic marks and the deposition of new marks occurs alongside major life stage transitions including gametogenesis and fertilization. However, despite occurring concomitantly with gametogenesis, the role of meiosis in epigenetic reprogramming has received little attention. To address this question, we use the model bryophyteMarchantia polymorpha. Following the haploid reproductive phase of this land plant, the expression of the paternal genome is silenced by the histone modification H3K27me3 in the short-lived diploid embryo. We show that imprinting is erased during meiosis, which occurs separately from gametogenesis and fertilization in Marchantia. The epigenetic reprogramming initiated during meiosis is completed in the meiotic spores where the chromatin landscape of the next haploid generation is establishedde novo. Hence, our findings illustrate a potential role for meiosis in epigenetic reprogramming that may be generalized to other sexually reproducing species.

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Nuclear transplantation, the conservation of the genome, and prospects for cell replacement

Cited by 11 publications

References 20 publications

From the raw bar to the bench: Bivalves as models for human health

From the raw bar to the bench: Bivalves as models for human health

Stem cells and tooth regeneration: prospects for personalized dentistry

Epigenetic reprogramming of imprinting at meiosis

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