Nucleic Acid Analysis and SDS—PAGE of Whole‒cell Proteins in<i>Bacillus</i>Taxonomy

Vos, Paul De

doi:10.1002/9780470696743.ch10

Cited by 3 publications

(2 citation statements)

References 73 publications

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“…With such a high number of strains to be examined, a method for identification at the species level has to be employed able to compare several strains at the same time. Among others comparison of protein patterns after SDS-PAGE is a cost-efficient method for the comparison of large groups of bacteria, and it can be used for an initial step in polyphasic characterization (Kersters 1985;de Vos 2002). Applying this approach, 11 protein similarity groups could be identified and a representative of each group was defined (Table I).…”

Section: Discussionmentioning

confidence: 99%

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

et al. 2011

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-This study was conducted to improve the knowledge about bacteria associated with honey bees, Apis mellifera carnica. In this survey, the diversity of Gram-negative non-fermenting bacteria isolated and cultivated from pollen loads, honey sac, freshly stored nectar, and honey was investigated. Bacteria were characterized by a polyphasic approach. Based on morphological and physiological characteristics and comparison of isolates protein patterns after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 11 protein similarity groups were established and confirmed by enterobacterial repetitive intergenic consensus PCR. One isolate, representing a protein similarity group (representative strain), was characterized in more detail by analysis of respiratory quinones, amplified ribosomal DNA restriction analysis, and 16S rDNA sequence analysis. Based on the results of these examinations, seven representative strains were identified as members of the genus Pseudomonas. The remaining representative strains were allocated to the genera Acinetobacter, Chryseobacterium, Stenotrophomonas, and Commamonas.bacterial diversity / PCR / honey / honey bees / 16S rDNA gene

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Section: Discussionmentioning

confidence: 99%

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

et al. 2011

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“…They reported insufficient dissimilarity in the species of B. subtilis when pairwise alignment of 16S rRNA gene sequences analysis was done (Hutsebaut et al, 2006). Although the use of 16S rDNA sequence analysis is very common in assigning strains into different taxa, it is difficult to detect any variation when distinguishing closely related organisms (De Vos, 2002;Fox et al, 1992) , 2015). Several studies have been conducted attempting to distinguish Bacillus species based on different phenotypic characteristics, such as fatty acid composition, pigmentation (Roberts et al, 1996), and analytical profile index (Logan and Berkeley, 1984).…”

Section: Discussionmentioning

confidence: 99%

Potential Biological Control Agents for Soilborne Fungal Pathogens in Tennessee Snap Bean Farms

Joshua¹,

Mmbaga²

2020

horts

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Fungi isolated from snap bean roots and rhizosphere soil where fungicides are not used included Fusarium oxysporum, Fusarium equiseti, Fusarium subglutinans, Fusarium camptoceras, Fusarium chlamydosporum, Fusarium verticillioides, Fusarium proliferatum, Fusarium acuminatum, Fusarium solani, Peyronellaea pinodella, Macrophomina phaseolina, and Glomerella guttata. Only P. pinodella, M. phaseolina, and F. oxysporum were isolated on symptomatic plants. These soilborne fungi are common pathogens of diverse host plants. Pathogenicity tests under controlled environment demonstrated that these fungi were pathogenic on snap beans. Subsequently, bacterial endophytes isolated from snap bean roots, papaya roots and stems, and dogwood stems were evaluated as potential biological control agents against these diverse fungi. All bacteria isolated, including Bacillus vallismortis (PS), Bacillus amyloliquefaciens (Psl), Bacillus subtilis (Prt), Bacillus thuringiensis (Y and IMC8), Enterobacter sp. (E), Stenotrophomonas sp. (B17A), and Serratia sp. (B17B) suppressed growth of the fungal pathogens in vitro and formed clear inhibition zones in petri dish dual cultures. Growth media taken from the inhibition zones suppressed growth of the fungal pathogens in the absence of the bacterial cells, suggesting that the bacteria released unidentified antagonistic biochemical substances into the media. This study constitutes an initial screening of endophytes as biological control agents against diverse fungal pathogens and forms a basis for the discovery of novel strains that can be further developed and integrated into disease management systems for diverse fungal pathogens. Isolates B. vallismortis (PS), B. amyloliquefaciens (Psl), B. subtilis (Prt), and B. thuringiensis (Y IMC8) exhibited the best performance as potential biological control agents paving the way for larger-scale in vivo studies and characterization of their interactions with fungal pathogens.

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Typing ofPantoea agglomeransisolated from colonies of honey bees (Apis mellifera) and culturability of selected strains from honey

et al. 2009

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Nucleic Acid Analysis and SDS—PAGE of Whole‒cell Proteins inBacillusTaxonomy

Cited by 3 publications

References 73 publications

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

Potential Biological Control Agents for Soilborne Fungal Pathogens in Tennessee Snap Bean Farms

Typing ofPantoea agglomeransisolated from colonies of honey bees (Apis mellifera) and culturability of selected strains from honey

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