Nucleic acid analysis on paper substrates (NAAPs): an innovative tool for Point of Care (POC) infectious disease diagnosis

Yadav, Supriya; Sharma, Niti Nipun; Akhtar, Jamil

doi:10.1039/d1an00214g

Cited by 18 publications

(6 citation statements)

References 117 publications

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“…As required by ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, and Deliverables to end users), microfluidic technologies are becoming essential tools. Since infectious diseases only require a tiny amount of biological fluid, microfluidics-based devices can be used at the POC for diagnosing and real-time monitoring infectious diseases [ 23 ]. The newly established wearable electronics using microfluidics, which combines flexible electronics with microfluidic devices, have attracted much interest in medical diagnosis.…”

Section: Microfluidicsmentioning

confidence: 99%

Microfluidics-Based Nanobiosensors for Healthcare Monitoring

2023

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Efficient healthcare management demands prompt decision-making based on fast diagnostics tools, astute data analysis, and informatics analysis. The rapid detection of analytes at the point of care is ensured using microfluidics in synergy with nanotechnology and biotechnology. The nanobiosensors use nanotechnology for testing, rapid disease diagnosis, monitoring, and management. In essence, nanobiosensors detect biomolecules through bioreceptors by modulating the physicochemical signals generating an optical and electrical signal as an outcome of the binding of a biomolecule with the help of a transducer. The nanobiosensors are sensitive and selective and play a significant role in the early identification of diseases. This article reviews the detection method used with the microfluidics platform for nanobiosensors and illustrates the benefits of combining microfluidics and nanobiosensing techniques by various examples. The fundamental aspects, and their application are discussed to illustrate the advancement in the development of microfluidics-based nanobiosensors and the current trends of these nano-sized sensors for point-of-care diagnosis of various diseases and their function in healthcare monitoring.

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Section: Microfluidicsmentioning

confidence: 99%

Microfluidics-Based Nanobiosensors for Healthcare Monitoring

2023

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“…Therefore, multiplex detection technology based on microfluidic platforms shows great potential for the diagnosis and mass screening of infectious diseases [ 19 , 20 ]. Although there are several reviews that comprehensively discuss microfluidic detection technologies for infectious disease diagnosis [ 21 , 22 , 23 ], few of them focus on microfluidic platforms for multiplex detection [ 21 ]. Therefore, this review summarizes recent advances in various microfluidic platforms for multiplex detection of infectious diseases ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Multiplex Detection of Infectious Diseases on Microfluidic Platforms

Chen

et al. 2023

Biosensors

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Infectious diseases contribute significantly to the global disease burden. Sensitive and accurate screening methods are some of the most effective means of identifying sources of infection and controlling infectivity. Conventional detecting strategies such as quantitative polymerase chain reaction (qPCR), DNA sequencing, and mass spectrometry typically require bulky equipment and well-trained personnel. Therefore, mass screening of a large population using conventional strategies during pandemic periods often requires additional manpower, resources, and time, which cannot be guaranteed in resource-limited settings. Recently, emerging microfluidic technologies have shown the potential to replace conventional methods in performing point-of-care detection because they are automated, miniaturized, and integrated. By exploiting the spatial separation of detection sites, microfluidic platforms can enable the multiplex detection of infectious diseases to reduce the possibility of misdiagnosis and incomplete diagnosis of infectious diseases with similar symptoms. This review presents the recent advances in microfluidic platforms used for multiplex detection of infectious diseases, including microfluidic immunosensors and microfluidic nucleic acid sensors. As representative microfluidic platforms, lateral flow immunoassay (LFIA) platforms, polymer-based chips, paper-based devices, and droplet-based devices will be discussed in detail. In addition, the current challenges, commercialization, and prospects are proposed to promote the application of microfluidic platforms in infectious disease detection.

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“…3–5 μPADs are simply fabricated without requiring cleanroom facilities, costly materials, or complex equipment. These advantages make μPADs more accessible compared to other microfluidic platforms, particularly in resource-limited areas, and usable in a wide range of applications, including healthcare and diagnostics, 6–17 environmental monitoring, 18–21 and food safety. 22–24…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5] μPADs are simply fabricated without requiring cleanroom facilities, costly materials, or complex equipment. These advantages make μPADs more accessible compared to other microfluidic platforms, particularly in resource-limited areas, and usable in a wide range of applications, including healthcare and diagnostics, [6][7][8][9][10][11][12][13][14][15][16][17] environmental monitoring, [18][19][20][21] and food safety. [22][23][24] μPADs are widely used in various applications to detect and analyze molecules, especially in point-of-care (PoC) systems, which provide rapid detection and analysis.…”

Section: Introductionmentioning

confidence: 99%