Nucleic Acid Oxidation in DNA Damage Repair and Epigenetics

Zheng, Guoliang; Fu, Ye; He, Chuan

doi:10.1021/cr400432d

Cited by 95 publications

(100 citation statements)

References 240 publications

(602 reference statements)

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“…FTO is a member of the AlkB subfamily of Fe II /α-ketoglutaratedependent dioxygenases, which has eight other family members in humans (ALKBH1-ALKBH8) and catalyzes the oxidation of diverse biological substrates (Kurowski et al 2003;Gerken et al 2007;Fu et al 2010;Zheng et al 2014). FTO was initially thought to work on 3-methylthymidine (3mT) in ssDNA (Gerken et al 2007) and 3-methyluracil (3mU) in ssRNA (Jia et al 2008).…”

Section: Erasers In Mammalsmentioning

confidence: 99%

RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation

2015

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N6 -methyladenosine (m 6 A) is the most prevalent and internal modification that occurs in the messenger RNAs (mRNA) of most eukaryotes, although its functional relevance remained a mystery for decades. This modification is installed by the m 6 A methylation "writers" and can be reversed by demethylases that serve as "erasers." In this review, we mainly summarize recent progress in the study of the m 6 A mRNA methylation machineries across eukaryotes and discuss their newly uncovered biological functions. The broad roles of m 6 A in regulating cell fates and embryonic development highlight the existence of another layer of epigenetic regulation at the RNA level, where mRNA is subjected to chemical modifications that affect protein expression.

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Section: Erasers In Mammalsmentioning

confidence: 99%

RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation

2015

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“…[1][2][3] The oxidative dealkylation mechanism catalyzed by AlkB, has been investigated extensively. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] The mechanism can be separated into two stages: The first stage involves the formation of a ferryl (Fe(IV)-oxo) intermediate, followed by the oxidation of the alkyl moiety on the damaged base.…”

Section: Introductionmentioning

confidence: 99%

Computational Investigation of O₂Diffusion Through an Intra–molecular Tunnel in AlkB; Influence of Polarization on O₂Transport

Torabifard

Cisneros

2017

Preprint

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“…Moreover, chloroplasts have a genome and a gene expression system that are targets of oxidation and mutagenesis by ROS (Wurtmann and Wolin, 2009;Zheng et al, 2014). Although chloroplasts have known quality control systems for oxidized DNA, proteins and lipids (Apel and Hirt, 2004), nothing is known about how they manage oxidized RNA.…”

Section: Introductionmentioning

confidence: 99%

Localized control of oxidized RNA

Zhan

Dhaliwal

Adjibade

et al. 2015

Journal of Cell Science

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The oxidation of biological molecules by reactive oxygen species (ROS) can render them inactive or toxic. This includes the oxidation of RNA, which appears to underlie the detrimental effects of oxidative stress, aging and certain neurodegenerative diseases. Here, we investigate the management of oxidized RNA in the chloroplast of the green alga Chlamydomonas reinhardtii. Our immunofluorescence microscopy results reveal that oxidized RNA (with 8-hydroxyguanine) is localized in the pyrenoid, a chloroplast microcompartment where CO 2 is assimilated by the Calvin cycle enzyme Rubisco. Results of genetic analyses support a requirement for the Rubisco large subunit (RBCL), but not Rubisco, in the management of oxidized RNA. An RBCL pool that can carry out such a 'moonlighting' function is revealed by results of biochemical fractionation experiments. We also show that human (HeLa) cells localize oxidized RNA to cytoplasmic foci that are distinct from stress granules, processing bodies and mitochondria. Our results suggest that the compartmentalization of oxidized RNA management is a general phenomenon and therefore has some fundamental significance.

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Nucleic Acid Oxidation in DNA Damage Repair and Epigenetics

Cited by 95 publications

References 240 publications

RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation

RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation

Computational Investigation of O₂Diffusion Through an Intra–molecular Tunnel in AlkB; Influence of Polarization on O₂Transport

Localized control of oxidized RNA

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Nucleic Acid Oxidation in DNA Damage Repair and Epigenetics

Cited by 95 publications

References 240 publications

RNA N6-methyladenosine methylation in post-transcriptional gene expression regulation

RNA N6-methyladenosine methylation in post-transcriptional gene expression regulation

Computational Investigation of O2Diffusion Through an Intra–molecular Tunnel in AlkB; Influence of Polarization on O2Transport

Localized control of oxidized RNA

Contact Info

Product

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RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation

RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation

Computational Investigation of O₂Diffusion Through an Intra–molecular Tunnel in AlkB; Influence of Polarization on O₂Transport