Nucleic Acids for Reagentless Biosensors

Cho, Eun Jeong; Lee, Joo Woon; Rajendran, Manjula; Ellington, Andrew D.

doi:10.1016/b978-044453125-4.50015-2

Cited by 5 publications

(3 citation statements)

References 188 publications

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“…Aptamers have proven to be useful as biosensors, in large measure because they can be readily adapted to a variety of sensor platforms and signal transduction schemes through the rational manipulation of their sequences and secondary structures (Bunka and Stockley, 2006;Cho et al, 2008;Deisingh, 2006;Nutiu and Li, 2005). Aptamer beacons have been designed that function similarly to molecular beacons, in that interaction with analytes leads to a separation of fluorophore and quencher, and subsequent ''light up'' signaling.…”

Section: Introductionmentioning

confidence: 99%

Kinetic optimization of a protein‐responsive aptamer beacon

Hall

Cater

Levy

et al. 2009

Biotech & Bioengineering

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Aptamers have been utilized as biosensors because they can be readily adapted to sensor platforms and signal transduction schemes through both rational design and selection. One highly generalizable scheme for the generation of the so-called aptamer beacons involves denaturing the aptamer with antisense oligonucleotides. For example, rational design methods have been utilized to adapt anti-thrombin aptamers to function as biosensors by hybridizing an antisense oligonucleotide containing a quencher to the aptamer containing a fluorescent label. In the presence of thrombin, the binding equilibrium is shifted, the antisense oligonucleotide dissociates, and the beacon lights up. By changing the affinity of the antisense oligonucleotide for the aptamer beacon, it has proven possible to change the extent of activation of the beacon. More importantly, modulating interactions between the antisense oligonucleotide and the aptamer strongly influences the kinetics of activation. Comparisons across multiple, designed aptamer beacons indicate that there is a strong inverse correlation between the thermodynamics of hybridization and the speed of activation, a finding that should prove to be generally useful in the design of future biosensors. By pre-organizing the thrombin-binding quadruplex within the aptamer the speed of response can be greatly increased. By integrating these various interactions, we were ultimately able to design aptamer beacons that were activated by threefold within 1 min of the addition of thrombin.

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Section: Introductionmentioning

confidence: 99%

Kinetic optimization of a protein‐responsive aptamer beacon

Hall

Cater

Levy

et al. 2009

Biotech & Bioengineering

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“…À cette distorsion de la nature du vêtement, s'ajoute celle qui consiste à en faire un lien et une entrave 61 : le vêtement-filet semble devenir réseau de chaînes, pareil aux liens infrangibles déployés par Héphaïstos pour immobiliser Prométhée. Si Eschyle précise que ces chaînes ne sont pas faites de métal 62 , le procédé rhétorique conduit à superposer sur l'objet des valeurs référentielles toujours plus éloignées. De filet, il devient « toile d'araignée », « toile tueuse de père » 63 .…”

Section: Les Rameaux Aiguisés Des Danaïdesunclassified

L’objet au théâtre avant le théâtre d’objets : dramaturgie et poétique de l’objet hybride dans les tragédies d’Eschyle

Noel¹

2011

agon

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“…This procedure is regarded as a simple, inexpensive and highly versatile method for the incorporation of components into film structures [22]. The advantages of a reagentless device fabricated using this approach is that it leads to a low cost biosensor which is convenient to use as no additional cofactor is required to be added to the sample solution [23], [24].…”

Section: Introductionmentioning

confidence: 99%

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications

Hughes

Pemberton

Fielden

et al. 2015

Sensors and Actuators B: Chemical

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Disclaimer UWE has obtained warranties from all depositors as to their title in the material deposited and as to their right to deposit such material. UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited. UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights.UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. The biosensor was fabricated by sequentially depositing the components on the surface of the transducer (MB-SPCE) in a layer-by-layer process, details of which are included in the paper. Each layer was optimized to construct the reagentless device.The biosensor was used in conjunction with amperometry in stirred solution using an applied potential of +0.1V (vs. Ag/AgCl). Optimum conditions for the analysis of glutamate were found to be: temperature, 35°C; phosphate buffer, pH 7 (0.75 mM, containing 0.05 M NaCl).The linear range of the reagentless biosensor was found to be 7.5 µM to 105 µM, and limit of detection was found to be 3 µM (based on n = 5, CV: 8.5% based on three times signal to noise) and the sensitivity was 0.39 nA/µM (± 0.025, coefficient of variation (CV) of 6.37%, n = 5). The response time of the biosensor was 20 -30 seconds.A food sample was analysed for monosodium glutamate (MSG). The endogenous content of MSG was 90.56 mg/g with a CV of 7.52%.The reagentless biosensor was also used to measure glutamate in serum. The endogenous concentration of glutamate was found to be 1.44 mM (n = 5), CV: 8.54%. The recovery of glutamate in fortified serum was 104% (n = 5), CV of 2.91%.

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Nucleic Acids for Reagentless Biosensors

Cited by 5 publications

References 188 publications

Kinetic optimization of a protein‐responsive aptamer beacon

Kinetic optimization of a protein‐responsive aptamer beacon

L’objet au théâtre avant le théâtre d’objets : dramaturgie et poétique de l’objet hybride dans les tragédies d’Eschyle

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications

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