Nucleocytoplasmic Interactions in Development of Amphibian Hybrids

Subtelny, Stephen

doi:10.1016/s0074-7696(08)60938-9

Cited by 23 publications

(2 citation statements)

References 142 publications

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“…Thus, they demonstrated that nuclei from embryos containing 8000 – 16,000 cells had the potential to promote enucleated eggs to develop into normal larvae. The cloning pioneers in 1952 conservatively concluded, “Although the method of nuclear transplantation should be valuable principally for the study of nuclear differentiation, it may also have other uses.” Some of the uses included the study of haploidy (Subtelny, 1958), hybrid incompatibility (Hennen, 1967; Subtelny, 1974; Gallien, 1979), cancer (Mc Kinnell et al, 1993), cellular aging (Orr et al, 1986; Lanza et al, 2000a), nuclear reprogramming (reviewed in Di Berardino, 1997a), and genomic imprinting (McGrath and Salter, 1984; Surani et al, 1984; Latham, 1999). But most importantly, Rana nuclear transplantation became the prototype for cloning metazoan animals, including those from insects, fish, amphibians, and mammals.…”

Section: Amphibiamentioning

confidence: 99%

“…The cloning pioneers in 1952 conservatively concluded, ''Although the method of nuclear transplantation should be valuable principally for the study of nuclear differentiation, it may also have other uses.'' Some of the uses included the study of haploidy (Subtelny, 1958), hybrid incompatibility (Hennen, 1967;Subtelny, 1974;Gallien, 1979), cancer (Mc Kinnell et al, 1993), cellular aging Lanza et al, 2000a), nuclear reprogramming (reviewed in Di Berardino, 1997a, and genomic imprinting (McGrath and Salter, 1984;Surani et al, 1984;Latham, 1999). But most importantly, pipette during which it gently breaks, and the broken cell is transof a metaphase II oocyte by pricking with a glass needle.…”

Section: Amphibiamentioning

confidence: 99%

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Animal Cloning – the route to new genomics in agriculture and medicine

Berardino

2001

Differentiation

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Section: Amphibiamentioning

confidence: 99%

Section: Amphibiamentioning

confidence: 99%

Animal Cloning – the route to new genomics in agriculture and medicine

Berardino

2001

Differentiation

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Amphidiploidy recovers the viability of hybrids of European and East Asian water frogs

Ohtani¹,

Miura²,

Kondo³

et al. 1997

J. Exp. Zool.

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Triploidy permits survival of an inviable amphibian hybrid

Elinson

Briedis

1981

Dev. Genet.

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Hybrids between species frequently arrest early in development. In the frog hybrid Rana catesbeiana female x Rana clamitans male, the embryo shows a characteristic development to an exogastrula which dies. This hybrid can be rescued by pressure suppression of the second polar body, which results in the addition of another haploid set of R catesbeiana chromosomes to the embryo. The triploid hybrid expresses genes from both species and can develop normally through metamorphosis. The results show that an R catesbeiana egg containing a full haploid set of R clamitans chromosomes is capable of development and that the usual developmental arrest caused by the R clamitans genome responds to chromosomal dosage. Key words: amphibian hybrids, exogastrulation, hybrid lethality, nucleocytoplasmic interactions, trip I o i d y I NTRO DU CTlO NFertilization produces new combinations of nuclei and cytoplasm, which by their interactions, direct early development. It is necessary that these interactions occur properly since alterations of early developmental events often lead to death of the embryo. This is a frequent result when hybrid embryos are produced. The nucleus from the sperm of one species is unable to interact correctly with the nucleus and cytoplasm of the egg of the second species, and the hybrid arrests at an early embryonic stage.On the one hand, we can consider lethal hybrids as developmental deadends, but on the other hand, we can view the hybrid embryo as one following an alternative developmental pathway. This pathway can be analyzed in comparison with the normal one in an attempt to dissect early nucleocytoplasmic interactions. With this aim in mind, hybrids between the green frog Rana clamitans and the bullfrog Rana catesbeiana have been studied. Offspring of the R catesbeiana female x R clamitans male cross arrest at gastrulation, while offspring of the R clamitans female x R catesbeiana male cross are viable and fertile [l-31. The normal development when the two nuclei are in R clamitans egg cytoplasm shows that the genomes from the

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Nucleocytoplasmic Interactions in Development of Amphibian Hybrids

Cited by 23 publications

References 142 publications

Animal Cloning – the route to new genomics in agriculture and medicine

Animal Cloning – the route to new genomics in agriculture and medicine

Amphidiploidy recovers the viability of hybrids of European and East Asian water frogs

Triploidy permits survival of an inviable amphibian hybrid

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