Nucleolar organiser regions in pathology

Egan, Mark; Crocker, J

doi:10.1038/bjc.1992.1

Cited by 86 publications

(34 citation statements)

References 91 publications

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“…Some members of this group are called Ag-NOR proteins because they are associated with the nucleolar organizer region (NOR) (1,2). The amount and distribution of Ag-NOR SSPs are currently used as markers of nucleolar activity and cell proliferation (3,4). Silver staining of nuclear extracts on Western blot demonstrated that B23, C23 and three unidentified proteins (29, 40 and 42 kDa) are the major SSPs during interphase; whereas UBF, RNA polymerase I largest subunit, and unidentified 50-kDa and 135-kDa proteins are the major SSPs during mitosis (5).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a new silver‐stainable protein SSP29, a member of the LRR family

et al. 1997

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Silver-stainable proteins (SSPs) are aspartic acid-rich nuclear proteins which are silver stained under very specific conditions. Using a degenerate oligodeoxynucleotide probe which codes for acidic amino acid residues, a cDNA for a new SSP, referred to as SSP29, has been isolated. The cDNA-derived amino acid sequence shows SSP29 has a molecular mass of 29 kDa, leucine-rich repeats (LRR) near the NH2-terminal region and acidic clusters at the COOHterminal portion, indicating that SSP29 is also a member of the LRR subfamily of acidic proteins which have been shown to be involved in antigen-mediated cellular responses, leukemogenesis and differentiation. SSP29 can be stained by Ag-NOR staining. SSP29 is expressed in all human tissues and cell lines tested, localized to nucleoplasm and translocated partially to the nucleoli after heat shock. Its interaction with RNA polymerase I suggests that SSP29 may participate in signal transduction that directs nucleolar activities by regulating ribosomal RNA biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a new silver‐stainable protein SSP29, a member of the LRR family

et al. 1997

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“…The proteins associated with the NORs, the so-called AgNOR proteins, are argyrophilic, acidic and non-histone (Fakan & Hernandez-Verdan, 1986) and may serve as a marker for rDNA transcription activity or of rDNA transcriptional potential (Dimova et al, 1982;Busch, 1984;Walker, 1988). Thus, AgNOR staining can also serve as a parameter of proliferation (Egan & Crocker, 1992 Iwanaga et al (1983). Adjuvant chemotherapy was given to 171 patients.…”

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Proliferative activity as a prognostic factor in Borrmann type 4 gastric carcinoma

et al. 1994

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“…2 AgNOR frequency is considered to be an index of transcriptional activity and hence a measure of the cellular mitotic potential. 3 The mean number of AgNORs in each nucleus (AgNOR count) is now widely used in tumour pathology as a diagnostic and prognostic marker, [4][5][6] because it can discriminate between malignant and benign or reactive proliferations, and has been shown to correlate well with the degree of aggressiveness and patient survival in many diVerent cancers, including haematological malignancies. [7][8][9][10] Multiple myeloma is a neoplasia of the B lymphocytic lineage characterised by lytic bone lesions, proliferation of plasma cells in the bone marrow and extramedullary sites, and high concentrations of a monoclonal immunoglobulin (M protein).…”

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Evaluation of argyrophilic nucleolar organiser regions (AgNORs) in multiple myeloma

Papadhimitriou

Daskalopoulou²,

Tsaftaridis³

et al. 2000

Journal of Clinical Pathology

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Conclusions-Unlike previous investigations, no clear prognostic value for the AgNOR count was found in multiple myeloma. Instead, the results indicate that the AgNOR count might be an index for M protein synthesis rate. This is consistent with other findings in tissues with low proliferative potential and high protein synthetic activity, and calls for a cautious interpretation of AgNORs in malignancies with similar features. (J Clin Pathol 2000;53:462-465)

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Nucleolar organiser regions in pathology

Cited by 86 publications

References 91 publications

Cloning and characterization of a new silver‐stainable protein SSP29, a member of the LRR family

Cloning and characterization of a new silver‐stainable protein SSP29, a member of the LRR family

Proliferative activity as a prognostic factor in Borrmann type 4 gastric carcinoma

Evaluation of argyrophilic nucleolar organiser regions (AgNORs) in multiple myeloma

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