Nucleolar organizer region enumeration in keratoacanthomas and squamous cell carcinomas of the skin

Kanitakis, Jean; Hoyo, E; Hermier, C; Chouvet, B; Thivolet, Lean

doi:10.1002/1097-0142(19920615)69:12<2937::aid-cncr2820691212>3.0.co;2-q

Cited by 27 publications

(10 citation statements)

References 32 publications

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“…In the skin, this is the case for malignant melanomas vs nevocellular (including Spitz) nevi,32 malignant vs benign fibrohistiocytic tumors,33 and SCCs vs keratoacanthomas. 34 The results of the present study did not show a significant difference in AgNOR counts between the two groups of SCCs stud¬ ied, which were otherwise (except for age, a factor that does not seem to influence the metastatic potential of SCC) comparable from the point of view of thickness and Broders' grade. This finding suggests that the in¬ trinsic proliferative potential of SCCs in OGRs is sim¬ ilar to that of control SCCs and is not enhanced by im¬ munosuppression.…”

Section: Resultscontrasting

confidence: 52%

Proliferation Characteristics of Cutaneous Squamous Cell Carcinomas Developing in Organ Graft Recipients

Hoyo

Kanitakis²,

Euvrard³

et al. 1993

Arch Dermatol

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Section: Resultscontrasting

confidence: 52%

Proliferation Characteristics of Cutaneous Squamous Cell Carcinomas Developing in Organ Graft Recipients

Hoyo

Kanitakis²,

Euvrard³

et al. 1993

Arch Dermatol

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“…The mean count of total AgNOR profi le area was 9.38 ± 0.25 μm 2 in epithelium adjacent to OSCC and 7.88 ± 0.27 μm 2 in normal oral mucosa and these values were in accordance with the results of previous studies. Total AgNOR profi le area showed an increased value because of the increase in number of AgNORs per nucleus in dysplastic cells as observed by Jean Kanitakis [14] , Giberto et al, [15] Sano K et al, [12] and Pifko J et al [16] …”

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confidence: 70%

Morphometric evaluation of AgNORs can help in identifying incipient cellular alterations in clinically normal-appearing oral mucosa adjacent to oral squamous cell carcinoma

Thippeswamy¹,

Einstein²,

Mujib³

et al. 2014

J Dent Res Sci Develop

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Background: The value of silver staining of nucleolar organizer region (AgNOR) counts as a diagnostic aid and has been reported in several neoplastic entities. Previous studies have proven the value of the morphometric evaluation of AgNOR in the detection of incipient cellular alterations. Materials and Methods:Computer-assisted morphometric analysis of AgNORs was performed in normal oral mucosa and mucosa adjacent to oral squamous cell carcinoma (OSCC). Morphometric values were compared between the study groups. Descriptive data were presented as mean ± SD; Student t-test was used for assessing the possible signifi cant difference between the study groups. Results:The results of the present study showed a signifi cant difference in all the four AgNOR-related parameters from epithelium adjacent to OSCC compared to the normal oral mucosa. Conclusion:The nucleolar organizing region (NOR)-related parameters assessed are biologically informative and easy to monitor routinely at a pathology laboratory. Further, AgNORs provide valuable data by which we can study the spectrum of nuclear changes in malignant and premalignant lesions.level and these should be regarded as the true interphase counterpart of the NORs present on the fi ve human acrocentric chromosomes, namely chromosome number 13, 14, 15, 21, and 22. The expression "AgNOR" designates, conveniently, the silver-stained NOR proteins. The argyrophilic silver staining of nucleolar organizer region (AgNOR) technique is remarkably specifi c as a means for the detection of NORs that demonstrates by virtue of silver binding to a wide array of NOR-associated Proteins (NORAPs). These include ribonucleic acid (RNA) polymerase I, C 23 protein (Nucleolin), and B 23 protein. [4] The patterns of NOR by size and distribution, as visualized by silver staining (AgNORs), are known to vary with different cell cycle stages. The number of individual dots may increase during the mitotic phase, whereas in the G 1 phase, there is a tendency for the dots to decrease in number, with a concomitant increase in AgNOR area. Computerized image analysis of the number of AgNORs, AgNOR area, size, and shape is an accurate and reproducible method to study the proliferative potential and cell stability of the tissue analyzed. IntroductionOral mucosa adjacent to squamous cell carcinoma (OSCC) is an interesting model for studying the biology of epithelia, which may have higher risk for malignant change in keeping with the premalignant hypothesis.[1] OSCC usually develops at a single site of the mucous membrane and expands in depth and laterally. Slaughter et al., [2] have suggested that carcinoma would arise from multifocal areas of precancerous change. Thus, lateral growth of the tumor may be due in part to progressive change to cancer of peripheral cells and not solely to expansion and destruction from preexisting malignant cells. Moreover, mucosa may be infl uenced by the paracrine effects of various growth factors produced by the cancer. [1] Nucleolar organizing regions (NORs) are loo...

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“…In keratoacanthomas and squamous cell carcinomas of the skin, AgNOR numbers differed significantly between groups; however, significant overlap between groups rendered AgNOR enumeration not a sufficiently discriminating criterion to be used in individual cases (33). It would be interesting to test the discriminative power of the morphometric parameters examined herein in cases (34) where AgNOR counts do not suffice.…”

Section: Discussionmentioning

confidence: 96%

AgNOR mark epithelial foci in malignant transformation in hamster cheek pouch carcinogenesis

Schwint

Folco

Morales

et al. 1996

J Oral Pathology Medicine

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Hamster cheek pouch mucosa is an accepted model of oral carcinogenesis. We herein examined the value of morphometric evaluation of silver-stained nucleolar organizer regions (AgNOR) in the detection of epithelial foci in malignant transformation following dimethyl-1,2-benzanthracene-induced carcinogenesis of hamster cheek pouch. AgNOR-related parameters were analyzed at different stages of the process of carcinogenesis (control epithelium, epithelium with no unusual microscopic features, "dysplastic" epithelium, exophytic and endophytic carcinomas). Morphometric evaluation of AgNOR revealed incipient cellular alterations which were not evident in routine preparations and contributed to the characterization of different stages of carcinogenesis in this model.

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Nucleolar organizer region enumeration in keratoacanthomas and squamous cell carcinomas of the skin

Cited by 27 publications

References 32 publications

Proliferation Characteristics of Cutaneous Squamous Cell Carcinomas Developing in Organ Graft Recipients

Proliferation Characteristics of Cutaneous Squamous Cell Carcinomas Developing in Organ Graft Recipients

Morphometric evaluation of AgNORs can help in identifying incipient cellular alterations in clinically normal-appearing oral mucosa adjacent to oral squamous cell carcinoma

AgNOR mark epithelial foci in malignant transformation in hamster cheek pouch carcinogenesis

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