Nucleoli and AgNORs in Hodgkin's disease.

Abstract

have demonstrated a significant difference in the mean numbers of silver stained nucleolar organiser regions (AgNORs) per neoplastic cell among different histological types. According to these findings the lymphoid predominant (LP) type is characterised by lower AgNOR scores than the other types (P < 0.05). The highest AgNOR numbers were found in the lymphocyte depleted type followed by the nodular sclerosing (NS) and mixed cellularity (MC) types. The aim of the present study was to evaluate the morphological characteristics of lymph node cells from Hodgkin's disease patients by measuring AgNORs in tissue sections and lymph node imprints. Sixty six lymph node touch imprints and 20 lymph node biopsy specimens were studied. Imprints were dried at room temperature, fixed in methanol/acetic acid (3:1), rinsed thoroughly with distilled water, and air dried repeatedly. These were put into 2N formic acid for 10 minutes to reduce the egress of silver from the nucleoli, and, to facilitate the measurement of nucleoli and AgNORs, rinsed again and impregnated with silver nitrate. Patients and methodsThe biopsy specimens were fixed in 10% neutral buffered formalin and embedded in paraffin wax. Sections (5 gm) were taken to

Identification of the AgNORs, PCNA and ck16 proteins in oral lichen planus lesions

“…Thus, in the present study, the results can be related also to increased synthesis activity of the keratinocytes that release many cytokines (Yang et al , 1986; Walsh et al , 1990; Porter et al , 1997). A higher AgNOR numbers in Hodgkin’s lymphoma cells with higher production of cytokines was reported (Mamaev et al , 1997).…”

Karyotypic dissection of Hodgkin's disease cell lines reveals ectopic subtelomeres and ribosomal DNA at sites of multiple jumping translocations and genomic amplification

“…A positive association between AgNOR activity and tumor stage in HD has been reported. 71,72 Regulation of rDNA transcription involves several tumorigenic pathways mediated through key molecules, including RB1 73 and TP53 74 for which a binding site is present downstream of Cb2b3. 46 …”

Análise quantitativa das AgNORs no carcinoma adenóide cístico intra-oral através da técnica de dupla marcação PCNA/AgNOR

“…A técnica de AgNOR tem sido amplamente utilizada como marcador de proliferação celular (4, 32) e na diferenciação entre tumores benignos e malignos do epitélio bucal (4) e de glândulas salivares (9,22 Valores percentuais referentes ao número das AgNORs dos núcleos PCNA positivos e PCNA negativos nos casos de CAC Tabela metabolismo ou na atividade transcricional da célula (5), ou ainda pode ser influenciado pela produção de citocinas (21). No carcinoma adenóide cístico, a análise simultânea de AgNOR e PCNA pode vir a esclarecer o papel da AgNOR nesta neoplasia como marcador de proliferação ou, simplesmente, da atividade metabólica das células tumorais.…”

“…Por outro lado, apesar de a quantificação das AgNORs estar fortemente relacionada à proliferação celular e ao prognóstico em vários tumores (4,5,7,21), os poucos estudos em tumores malignos de glândula salivar, em especial no carcinoma adenóide cístico (CAC), apresentam resultados que deixam ainda dúvidas quanto ao papel das NORs nestas lesões (12,13,36).…”