2021
DOI: 10.1016/j.jviromet.2021.114064
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Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?

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Cited by 18 publications
(23 citation statements)
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“…The N-protein structure [ 22 ], unlike the S-protein RBD [ 19 , 20 , 23 ], does not show cross-reactivity with anti-dengue antibodies [ 24 , 25 , 26 , 27 ] and is not targeted by the current approved COVID-19 vaccines [ 28 ]. Thus, the utility of N-protein immunoassays, rather than S-protein immunoassays, should be considered to assess the exposure to SARS-CoV-2 infection and to estimate the incidence of COVID-19 disease in dengue endemic populations.…”
Section: Introductionmentioning
confidence: 99%
“…The N-protein structure [ 22 ], unlike the S-protein RBD [ 19 , 20 , 23 ], does not show cross-reactivity with anti-dengue antibodies [ 24 , 25 , 26 , 27 ] and is not targeted by the current approved COVID-19 vaccines [ 28 ]. Thus, the utility of N-protein immunoassays, rather than S-protein immunoassays, should be considered to assess the exposure to SARS-CoV-2 infection and to estimate the incidence of COVID-19 disease in dengue endemic populations.…”
Section: Introductionmentioning
confidence: 99%
“…For SARS-CoV-2, several studies had reported the use of ELISA to detect antibodies against N protein of SARS-CoV and the sensitivity and specificity of such test ranged from 90 to 94%, and, above 97%, respectively ( Table 11 ) [202] , [203] . Compared to ELISA assay that was aimed to detect antibodies like IgG against the N protein of SARS-CoV-2 [202] , [203] , the use of ELISA test that was focused to detect antibodies against S protein of SARS-CoV-2 showed wide range values of sensitivity and specificity, according to the findings reported from various studies [199] , [204] , [205] , [206] , [207] .…”
Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning
confidence: 99%
“…For SARS-CoV-2, several studies had reported the use of ELISA to detect antibodies against N protein of SARS-CoV and the sensitivity and specificity of such test ranged from 90 to 94%, and, above 97%, respectively ( Table 11 ) [202] , [203] . Compared to ELISA assay that was aimed to detect antibodies like IgG against the N protein of SARS-CoV-2 [202] , [203] , the use of ELISA test that was focused to detect antibodies against S protein of SARS-CoV-2 showed wide range values of sensitivity and specificity, according to the findings reported from various studies [199] , [204] , [205] , [206] , [207] . Some studies showed that S protein-based ELISA test was less sensitive to detect IgG and IgA against SARS-CoV-2 (<70%) [206] , [207] while few other studies reported that such test was highly sensitive (at least 90%) to detect IgG, IgA, or IgM against the virus S antigen [199] , [204] , [205] , [208] .…”
Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning
confidence: 99%
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“…Under the current circumstances, where millions of viral infections exist, it is undeniable that vaccination is of vital importance, and immunoassays have taken a particularly relevant role in identifying and monitoring patients’ immune responses over time. Therefore, several research groups have proposed various COVID-19-related immunoassays, which are mainly based on viral antigens, such as the spike protein [ 13 ], receptor-binding domain (RBD) [ 14 ], and nucleoprotein [ 15 ], and include different strategies ranging from the traditional enzyme-linked immunoassay (ELISA) to more complex microfluidic immunoassays [ 16 , 17 , 18 ].…”
Section: Introductionmentioning
confidence: 99%