Nucleoside and Ribonucleic Acid Metabolism in Isolated Bone Cells

Peck, William A.; Messinger, Kirk

doi:10.1016/s0021-9258(18)63128-x

Cited by 50 publications

(1 citation statement)

References 35 publications

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“…Cultured bone cells stimulated with insulin show an increase in [l4C]uridine uptake, presumably no effect on the intracellular UTP pool size and an increase in [l4C]uridine incorporation into RNA. Essential amino acid deficiency and treatment of the cultures with inhibitors of protein synthesis had no effect on insulin stimulation of radiolabeled uridine uptake and incorporation into RNA (Peck and Messinger, 1970). It appears that no uniform action of insulin on uridine uptake and incorporation into RNA can be extrapolated from the above studies and our data.…”

Section: Discussionmentioning

confidence: 95%

Amino acid dependent and independent insulin stimulation of cartilage metabolism

Beuttel¹,

Eisenbarth²,

Lebovitz³

1977

Biochemistry

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The effects of insulin on embryonic chicken cartilage in organ culture and the dependence of these effects on essential amino acids have been studied. In the presence of all essential amino acids, insulin: (1) increases 2-deoxy-D-glucose and alpha-aminoisobutyric acid uptake; (2) increases [5(-3H] uridine flux into uridine metabolites and the intracellular UTP pool; (3) expands the size of the intracellular UTP pool; (4) does not change the specific activity of the UTP pool; and (5) stimulates RNA, proteoglycan, and total protein synthesis. In lysine (or other essential amino acid)-deficient medium, the effects of insulin are different. While insulin stimulates incorporation of [5(-3)H] uridine into RNA, it does so by increasing the specific activity of the UTP pool without increasing RNA synthesis. Insulin stimulates 2-deoxy-D-glucose and alpha-aminoisobutyric acid uptake but no longer stimulates proteoglycan, total protein, or RNA synthesis or expands the size of the UTP pool. These data indicate that there are amino acid dependent and independent effects of insulin on cartilage. Transport processes are amino acid independent, while synthetic processes are amino acid dependent.

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Section: Discussionmentioning

confidence: 95%

Amino acid dependent and independent insulin stimulation of cartilage metabolism

Beuttel¹,

Eisenbarth²,

Lebovitz³

1977

Biochemistry

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Growth‐Promoting Actions of Peptide Hormones

Tata

1976

Novartis Foundation Symposia

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Impairment of osteophyte formation in hyperglycemic patients with type II diabetes mellitus and knee osteoarthritis

Horn

Bradley

Brandt

et al. 1992

Arthritis & Rheumatism

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Objective. Since insulin is a potent growth factor for connective tissue, the present study was designed to investigate whether radiographic features of knee osteoarthritis (OA) in patients with poorly controlled, insulin-resistant type I1 diabetes mellitus differ from those in nondiabetic controls with knee OA.Methods. Radiographs from 25 female patients with diabetes and knee OA were compared with those from 48 female controls who were similar with respect to age, weight, and duration of OA symptoms.Results. Although the 2 groups were similar with respect to the frequency and severity of joint space narrowing, subchondral sclerosis, and geodes, osteophytes were less common in the patients with diabetes (P = 0.044), and spurring, when present, tended to be "marked" less often in the diabetic patients than in the controls.Conclusion. The data suggest that diminished

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Nucleoside and Ribonucleic Acid Metabolism in Isolated Bone Cells

Cited by 50 publications

References 35 publications

Amino acid dependent and independent insulin stimulation of cartilage metabolism

Amino acid dependent and independent insulin stimulation of cartilage metabolism

Growth‐Promoting Actions of Peptide Hormones

Impairment of osteophyte formation in hyperglycemic patients with type II diabetes mellitus and knee osteoarthritis

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