Nucleoside reverse transcriptase inhibitors and their phosphorylated metabolites in human immunodeficiency virus-infected human matrices

Mass spectrometry in the quantitative analysis of therapeutic intracellular nucleotide analogs

“…Concomitantly, the MS signal increased 5-to 10-fold (Cai, Song, & Yang, 2002). In contrast to previous reports (Lai, Wang, & Cai, 2008), a clear relation between column diameter and applied N,N-DMHA …”

“…A review on the analysis of nucleoside reverse transcriptase inhibitors (NRTIs) and their phosphorylated metabolites in HIV-infected matrices has recently been published by Lai, Wang, and Cai (2008). The analytical methods can be divided into direct and indirect methods.…”

Molecularly imprinted polymer for analysis of zidovudine and stavudine in human serum by liquid chromatography–mass spectrometry

“…Current therapy of acquired immunodeficiency syndrome (AIDS) consists in a combination of several antiretroviral including nucleoside reverse transcriptase inhibitors (NRTIs) [1,2]. Zidovudine and stavudine are the most widely used NRTIs in AIDS treatment [3].…”

“…Zidovudine and stavudine are the most widely used NRTIs in AIDS treatment [3]. Analytical methods, including either high performance liquid chromatography (HPLC) [1][2][3][4][5][6][7][8][9] or capillary electrophoresis (CE) [1,10] have been used to quantify zidovudine (AZT) or stavudine (d4T) in biological media. Most of these methods used solid-phase extraction (SPE) as preliminary treatment.…”

“…For example, pharmaceutical compounds such abacavir [18], methotrexate [19], hydroxyzine [20], alfuzosin [15] were extracted from serum or urine. However, to the best of our knowledge, there is a few of MIPs developed for the nucleotides prodrugs [21,22] and for the NRTIs [1,18].…”