Nucleoskeletal stiffness regulates stem cell migration and differentiation through lamin A/C

Chen, Liujing; Jiang, Fulin; Qiao, Yini; Li, Hong; Wei, Zhangming; Huang, Tu; Lan, Jingxiang; Xia, Yue; Li, Juan

doi:10.1002/jcp.26336

Cited by 18 publications

(14 citation statements)

References 83 publications

(129 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The processes of MSCs’ migration to injured tissue and differentiation into specific lineage are paramount in bone formation and remodeling. These processes are highly determinate by lamin A/C implication for their correct accomplishment [ 43 ].…”

Section: Lamin A/c and Mscsmentioning

confidence: 99%

See 1 more Smart Citation

Crucial Role of Lamin A/C in the Migration and Differentiation of MSCs in Bone

Alcorta-Sevillano

Macías

Rodrı́guez

et al. 2020

Cells

View full text Add to dashboard Cite

Lamin A/C, intermediate filament proteins from the nuclear lamina encoded by the LMNA gene, play a central role in mediating the mechanosignaling of cytoskeletal forces into nucleus. In fact, this mechanotransduction process is essential to ensure the proper functioning of other tasks also mediated by lamin A/C: the structural support of the nucleus and the regulation of gene expression. In this way, lamin A/C is fundamental for the migration and differentiation of mesenchymal stem cells (MSCs), the progenitors of osteoblasts, thus affecting bone homeostasis. Bone formation is a complex process regulated by chemical and mechanical cues, coming from the surrounding extracellular matrix. MSCs respond to signals modulating the expression levels of lamin A/C, and therefore, adapting their nuclear shape and stiffness. To promote cell migration, MSCs need soft nuclei with low lamin A content. Conversely, during osteogenic differentiation, lamin A/C levels are known to be increased. Several LMNA mutations present a negative impact in the migration and osteogenesis of MSCs, affecting bone tissue homeostasis and leading to pathological conditions. This review aims to describe these concepts by discussing the latest state-of-the-art in this exciting area, focusing on the relationship between lamin A/C in MSCs’ function and bone tissue from both, health and pathological points of view.

show abstract

Section: Lamin A/c and Mscsmentioning

confidence: 99%

“…It is known that cells with higher migration ability tend to have enhanced differentiation potential, but the optimum amount of lamin A/C is different for MSCs’ migration and differentiation [ 43 ]. During bone formation, MSCs migrate until they reach the bone.…”

Section: Lamin A/c and Mscsmentioning

confidence: 99%

Crucial Role of Lamin A/C in the Migration and Differentiation of MSCs in Bone

Alcorta-Sevillano

Macías

Rodrı́guez

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…Finally, the nuclear lamins, and lamin A/C in particular, represent an important determinant of the viscoelastic properties of the nucleus and the entire cell [30]. Higher levels of lamin A, which typically occur in differentiated cells, have been found to promote stiffness in a way that impedes migration in a restrictive 3D environment but enhances stress resistance and cell survival for a broad array of cell types [31,32]. Moreover, the maintenance of low levels of lamin A was recently found to be relevant in granulocytes undergoing chemokineinduced chemotaxis and cell squeezing and extravasation across endothelial barriers under physiological conditions [33].…”

Section: Cytoplasmic and Nuclear Intermediate Filamentsmentioning

confidence: 99%

Non-canonical processes that shape the cell migration landscape

Majumdar

Steen

Parent

2019

Current Opinion in Cell Biology

View full text Add to dashboard Cite

Migration is a vital, intricate and multi-faceted process that involves the entire cell, entails the integration of multiple external cues and, at times, necessitates high-level coordination among fields of cells that can be physically attached or not, depending on the physiological setting. Recent advances have highlighted the essential role of cellular components that have not been traditionally considered when studying cell migration. This review details how much we recently learned by studying the role of intermediate filaments, the nucleus, extracellular vesicles and mitochondria during cell migration.

show abstract

“…Stem cells with higher migration ability are more sensitive to force and can raise the amount of lamin A/C for later osteogenic differentiation. 73 Other evidence indicated that some factors were connected to both stem cell migration and differentiation. While the involvement of cell-generated force in the connection remained unclear.…”

Section: Stem-cell Migration and Lineage Differentiationmentioning

confidence: 99%

Perspective into the regulation of cell‐generated forces toward stem cell migration and differentiation

Chen

Huang

Qiao

et al. 2018

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

Stem cells are promising candidates for cell-based therapies in diverse conditions including regenerating damaged tissues, treating inflammation in virtue of sepsis, acute renal failure, and cardiovascular disease. Advancement of these therapies relies on the ability to guide stem cells to migrate directly and differentiate towards specific cell phenotypes. During the past decade, many researchers have demonstrated that exogenous applied forces could significantly affect the migration and lineage differentiation of stem cells. Besides, recent advances have highlighted the critical role of internal forces due to cellmatrix interaction in the function of stem cells. Stem cells can generate contractile forces to sense the mechanical properties of cell-generated force microenvironment, and thereby perceive mechanical information that directs broad aspects of stem cell functions, including migration and lineage commitment. In the review, we recount the cell-generated force microenvironment of stem cells and discuss the interactions between cell-generated forces with migration and differentiation of stem cells. We also summarize key experimental evidence of a tight linkage between migration and lineage differentiation of stem cells and pose important unanswered questions in this field.

show abstract

Nucleoskeletal stiffness regulates stem cell migration and differentiation through lamin A/C

Cited by 18 publications

References 83 publications

Crucial Role of Lamin A/C in the Migration and Differentiation of MSCs in Bone

Crucial Role of Lamin A/C in the Migration and Differentiation of MSCs in Bone

Non-canonical processes that shape the cell migration landscape

Perspective into the regulation of cell‐generated forces toward stem cell migration and differentiation

Contact Info

Product

Resources

About