Nucleotide adsorption–desorption behaviour of boronic acid functionalized uniform-porous particles

RNA-sensitive N-isopropylacrylamide/vinylphenylboronic acid random copolymer

“…In the chromatographic applications involving the use of boronic acid-functionalized supports for the isolation of diol-carrying biomolecules such as nucleotides, RNA, the most efficient binding of biomolecules to the support materials have been usually observed at pH values close to pK a of boronic acid (i.e., between pH 8 and 9). [27][28][29][30][31][32][33][34] In our study, the appreciable LCST decrease at pH 9 is probably explained by effective binding of RNA onto the NIPA-co-VPBA copolymer. Here, an increase in the amount of RNA bound onto the copolymer is also expected with increasing RNA concentration.…”

“…[25,26] On the other hand, boronic acid is a commonly used ligand particularly in chromatographic studies involving the separation of diol-carrying biomolecules. [27][28][29][30][31][32][33][34] These chromatographic applications have been usually based on the complex formation between diol groups of the target biomolecule and boronic acid groups of the support material. [27][28][29][30][31][32][33][34] Based on the same interaction, boronic acid-carrying thermosensitive polymers were used in the detection of diol-carrying biomolecules.…”

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

“…Since emulsion prepared seeds are generally in the submicron range, they are suitable for obtaining particles up to 10 µm in diameter after the suspension polymerization stage [68][69]. On the other hand, dispersion polymerized seeds can be in the range of 1-20 µm, thus 10-200 µm particles can be obtained in the suspension polymerization stage [70][71][72][73][74][75][76][77][78][79]. Note that a volume enlargement of 10 6 times would be needed for a 1 µm seed to be swollen by the new monomer(s) to 100 µm.…”

Synthesis of monodisperse poly(styrene-co-divinylbenzene) microspheres with binary porous structures and application in high-performance liquid chromatography

“…Different kinds of multistage methods, such as activated swelling [10,11], seeded emulsion polymerization [12], precipitation polymerization [13], template imprinting [14,15], and membrane techniques [16,17] have been developed to prepare the monodisperse porous polystyrene particles. Preparation conditions related to the porosity formation, surface modification, structure stability, and swelling homogeneity have also been extensively investigated [18][19][20][21][22].…”