Nucleotide Identification in DNA Using Dielectrophoresis Spectroscopy

Abstract

We show that negative dielectrophoresis (DEP) spectroscopy is an effective transduction mechanism of a biosensor for the detection of single nucleotide polymorphism (SNP) in a short DNA strand. We observed a frequency dependence of the negative DEP force applied by interdigitated electrodes to polystyrene microspheres (PM) with respect to changes in both the last and the second-to-last nucleotides of a single-strand DNA bound to the PM. The drift velocity of PM functionalized to single-strand DNA, which is proportional to the DEP force, was measured at the frequency range from 0.5 MHz to 2 MHz. The drift velocity was calculated using a custom-made automated software using real time image processing technique. This technology for SNP genotyping has the potential to be used in the diagnosis and the identification of genetic variants associated with diseases.

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

“…Compared with traditional catalytic hairpin assembly (CHA) components, UECHA allows for direct and quantitative measurement of AD miRNAs in 1 μL samples, with a resolution of 3.55 × 10 −15 M. This visualization analysis of ultratrace biomarkers based on acoustic enrichment and promotion provides a new perspective for rapid and highly sensitive clinical detection of Alzheimer’s disease. According to the research of Fleming Dackson Gudaguti et al [ 99 ], negative dielectrophoresis (DEP) spectroscopy serves as an effective transduction mechanism for a biosensor designed to detect single nucleotide polymorphisms (SNPs) in short DNA strands. They observed a frequency-dependent negative DEP force applied by interdigitated electrodes to polystyrene microspheres (PMs) in response to changes in both the last and second-to-last nucleotides of a single-strand DNA bound to the PM.…”

Liquid Biopsy and Dielectrophoretic Analysis—Complementary Methods in Skin Cancer Monitoring

“…Depending on the employed external stimuli, there is a wide range of cell manipulation techniques currently in use, including dielectrophoresis (DEP). DEP is a non-invasive manipulation method suitable for measurements at the micro-and nanoscale level, with a great applicability potential in a large variety of bioparticles, including several types of tumor cells [73], CTCs [74], DNA [75], RNA [76] and other small particles, such as exosomes [77].…”

Editorial for the Special Issue on Particles Separation in Microfluidic Devices

“…Five papers [1-5] and a review article [6] present (1) passive microfluidic techniques using inertial focusing [1,6], deterministic lateral displacement (DLD) [2,3], and hydrodynamic methods [4,5]. The remaining papers [7][8][9][10][11][12] and a review article [13] cover (2) active microfluidic techniques using electric [7][8][9], acoustic [10], magnetic [11,12], and optical forces [13].() Passive microfluidic technique: Bogseth et al proposed a co-flow inertial microfluidic device that is tunable in multiple ways for adaptation to different application requirements [1]. They evaluated flow rate, flow rate ratio, and output resistance ratio to flexibly tune the cutoff size of the device and separation performance even after the devices are fabricated.…”

“…Five papers [1-5] and a review article [6] present (1) passive microfluidic techniques using inertial focusing [1,6], deterministic lateral displacement (DLD) [2,3], and hydrodynamic methods [4,5]. The remaining papers [7][8][9][10][11][12] and a review article [13] cover (2) active microfluidic techniques using electric [7][8][9], acoustic [10], magnetic [11,12], and optical forces [13].…”

“…The proposed model is used for a parametric study to investigate the effect of parameters on the performance of the microfluidic device. Gudagunti et al used negative dielectrophoresis (DEP) spectroscopy as an effective transduction mechanism of a biosensor to accurately detect single nucleotide polymorphism (SNP) in a short DNA strand [9]. Clark et al demonstrated real-time monitoring of voltage measurements and immediate, corresponding adjustments to acoustic trapping frequency to improve their acoustic differential extraction [10].…”