Nucleotide sequence analysis of endogenous murine leukemia virus-related proviral clones reveals primer-binding sites for glutamine tRNA

Nikbakht, Kave; Ou, Chin Yih; Boone, Lawrence R.; Glover, Paul L.; Yang, Wei‐Pang

doi:10.1128/jvi.54.3.889-893.1985

Cited by 37 publications

(28 citation statements)

References 39 publications

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“…A stretch of 18 bp, located immediately downstream of the 5' U5 region, was examined to determine PBSs for the putative MuERVs. The conserved PBS sequences for tRNA Proline (P) and tRNA Glutamine (Q) were used as references (Harada, Peters, and Dahlberg, 1979;Nikbakht et al, 1985).…”

Section: Pbs Analysismentioning

confidence: 99%

Genome-wide expression profiles of endogenous retroviruses in lymphoid tissues and their biological properties

et al. 2008

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Endogenous retroviruses (ERVs) constitute approximately 8-10% of the human and mouse genome. Some autoimmune diseases are attributed to the altered expression of ERVs. In this study, we examined the ERV expression profiles in lymphoid tissues and analyzed their biological properties. Tissues (spleen, thymus, and lymph nodes [axillary, inguinal, and mesenteric]) from C57BL/6J mice were analyzed for differential murine ERV (MuERV) expression by RT-PCR examination of polymorphic U3 sequences. Each tissue had a unique profile of MuERV expression. A genomic map identifying 60 putative MuERVs was established using 22 unique U3s as probes and their biological properties (primer binding site, coding potential, transcription regulatory element, tropism, recombination event, and integration age) were characterized. Interestingly, 12 putative MuERVs retained intact coding potentials for all three polypeptides essential for virus assembly and replication. We suggest that MuERV expression is differentially regulated in conjunction with the transcriptional environment of individual lymphoid tissues.

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Section: Pbs Analysismentioning

confidence: 99%

Genome-wide expression profiles of endogenous retroviruses in lymphoid tissues and their biological properties

et al. 2008

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“…Furthermore, analysis of pol mutants of Moloney MLV demonstrated that the presence in MLV virions of tRNA Pro correctly annealed to the PBS is independent of products of the pol gene (13). Additional indications for a less stringent use of tRNA primers in MLV come from sequencing of MLV-related viruses endogenous to the murine genome carrying PBSs that match tRNA Gln (10,31).…”

mentioning

confidence: 99%

Replication and Pathogenicity of Primer Binding Site Mutants of SL3-3 Murine Leukemia Viruses

Lund

Schmidt

Luz³

et al. 1999

J Virol

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Retroviral reverse transcription is primed by a cellular tRNA molecule annealed to an 18-bp primer binding site sequence. The sequence of the primer binding site coincides with that of a negatively acting cis element that mediates transcriptional silencing of murine leukemia virus (MLV) in undifferentiated embryonic cells. In this study we test whether SL3-3 MLV can replicate stably using tRNA primers other than the cognate tRNAPro and analyze the effect of altering the primer binding site sequence to match the 3′ end of tRNA1 Gln, tRNA3 Lys, or tRNA1,2 Arg in a mouse pathogenicity model. Contrary to findings from cell culture studies of primer binding site-modified human immunodeficiency virus type 1 and avian retroviruses, our findings were that SL3-3 MLV may stably and efficiently replicate with tRNA primers other than tRNAPro. Although lymphoma induction of the SL3-3 Lys3 mutant was significantly delayed relative to that of the wild-type virus, molecular tumor analysis indicated that all the primer binding site-modified viruses induce T-cell lymphomas similar to those induced by the wild-type virus in terms of frequencies of genomic rearrangements within the T-cell receptor β-chain, the immunoglobulin κ light chain, and the c-myc locus. Whereas none of the mutants were found to revert to tRNAProprimer utilization, in two tumors resulting from the injection of the SL3-3 Lys3 mutant the primer binding site was altered to match that of a new primer species, tRNA1,2 Lys. In addition, recombination with endogenous viruses resulting in the generation of recombinant viruses carrying a glutamine primer binding site was detected in the majority of the tumors induced by the SL3-3 Lys3 mutant as well as in two tumors induced by wild-type SL3-3 and the SL3-3 Arg1,2 mutant.

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“…The remaining endogenous viruses, present at between 12 and 30 copies (44), have not yet been systematically studied, although a number of isolated proviruses have been examined in varying detail (28,30,52). These viruses differ from the inducible ecotropic and xenotropic viruses in a number of respects, including long terminal repeat (LTR) size (29, 45), tRNA primer-binding site (42), and the sequences of their eait' genes (28,32). Studies of cloned proviruses have not revealed major differences among the nonecotropic proviruses; however, analyses of genomic DNA have provided suggestive evidence for the presence of two classes of nonecotropic, nonxenotropic viruses differing slightly in the distribution of restriction endonuclease cleavage sites (15,55).…”

mentioning

confidence: 99%

The four classes of endogenous murine leukemia virus: structural relationships and potential for recombination

Stoye¹,

Coffin²

1987

J Virol

222

155

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The process by which leukemogenic viruses are generated during the lifetime of certain strains of mice is poorly understood. We have therefore set out to define all the murine leukemia virus-related endogenous proviruses of HRS/J mice. We have cloned 34 different proviral fragments and their flanking cellular sequences. These have been characterized by restriction enzyme analysis, by fingerprinting in vitro-synthesized RNA, and by DNA sequencing. We conclude that all the proviruses can be assigned into one of four different classes: the previously characterized ecotropic, xenotropic, and polytropic viruses, as well as a new class we have termed modified polytropic viruses. The xenotropic, polytropic, and modified polytropic classes are closely related to one another, but as a group they differ considerably from the ecotropic class. Sequence analyses show that both polytropic and modified polytropic sequences can contribute env sequences to recombinant viruses.

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Nucleotide sequence analysis of endogenous murine leukemia virus-related proviral clones reveals primer-binding sites for glutamine tRNA

Cited by 37 publications

References 39 publications

Genome-wide expression profiles of endogenous retroviruses in lymphoid tissues and their biological properties

Genome-wide expression profiles of endogenous retroviruses in lymphoid tissues and their biological properties

Replication and Pathogenicity of Primer Binding Site Mutants of SL3-3 Murine Leukemia Viruses

The four classes of endogenous murine leukemia virus: structural relationships and potential for recombination

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